Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia
Crinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015–2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregul...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-10-01
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| Series: | Mycobiology |
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| Online Access: | http://dx.doi.org/10.1080/12298093.2019.1682448 |
| _version_ | 1818307859514916864 |
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| author | Abd Rahim Huda-Shakirah Yee Jia Kee Abu Bakar Mohd Hafifi Nurul Nadiah Mohamad Azni Latiffah Zakaria Masratul Hawa Mohd |
| author_facet | Abd Rahim Huda-Shakirah Yee Jia Kee Abu Bakar Mohd Hafifi Nurul Nadiah Mohamad Azni Latiffah Zakaria Masratul Hawa Mohd |
| author_sort | Abd Rahim Huda-Shakirah |
| collection | DOAJ |
| description | Crinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015–2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on C. asiaticum and H. littoralis. Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-α) gene, the causal pathogen was identified as Macrophomina phaseolina. Phylogenetic analysis of combined dataset of ITS and TEF1-α grouped the isolates studied with other isolates of M. phaseolina from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts. |
| first_indexed | 2024-12-13T07:05:04Z |
| format | Article |
| id | doaj.art-ef756c36e5054d16b1364c640b9b6846 |
| institution | Directory Open Access Journal |
| issn | 1229-8093 2092-9323 |
| language | English |
| last_indexed | 2024-12-13T07:05:04Z |
| publishDate | 2019-10-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycobiology |
| spelling | doaj.art-ef756c36e5054d16b1364c640b9b68462022-12-21T23:55:51ZengTaylor & Francis GroupMycobiology1229-80932092-93232019-10-0147440841410.1080/12298093.2019.16824481682448Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in MalaysiaAbd Rahim Huda-Shakirah0Yee Jia Kee1Abu Bakar Mohd Hafifi2Nurul Nadiah Mohamad Azni3Latiffah Zakaria4Masratul Hawa Mohd5Universiti Sains MalaysiaUniversiti Sains MalaysiaUniversiti Sains MalaysiaUniversiti Sains MalaysiaUniversiti Sains MalaysiaUniversiti Sains MalaysiaCrinum asiaticum and Hymenocallis littoralis, commonly known as spider lilies are bulbous perennial and herbaceous plants that widely planted in Malaysia as ornamental. During 2015–2016, symptom of leaf blight was noticed on the hosts from several locations in Penang. The symptom appeared as irregular brown to reddish lesions surrounded by yellow halos. As the disease progressed, the infected leaves became blighted, dried, and fell off with the presence of black microsclerotia and pycnidia on the lesions parts. The present study was conducted to investigate the causal pathogen of leaf blight on C. asiaticum and H. littoralis. Based on morphological characteristics and DNA sequences of internal transcribed spacer (ITS) region and translation elongation factor 1-alpha (TEF1-α) gene, the causal pathogen was identified as Macrophomina phaseolina. Phylogenetic analysis of combined dataset of ITS and TEF1-α grouped the isolates studied with other isolates of M. phaseolina from GenBank. The grouping of the isolates was supported by 96% bootstrap value. Pathogenicity test proved the role of the fungus in causing leaf blight on both hosts.http://dx.doi.org/10.1080/12298093.2019.1682448macrophomina phaseolinaleaf blightcrinum asiaticumhymenocallis littoralis |
| spellingShingle | Abd Rahim Huda-Shakirah Yee Jia Kee Abu Bakar Mohd Hafifi Nurul Nadiah Mohamad Azni Latiffah Zakaria Masratul Hawa Mohd Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia Mycobiology macrophomina phaseolina leaf blight crinum asiaticum hymenocallis littoralis |
| title | Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia |
| title_full | Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia |
| title_fullStr | Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia |
| title_full_unstemmed | Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia |
| title_short | Identification and Characterization of Macrophomina phaseolina Causing Leaf Blight on White Spider Lilies (Crinum asiaticum and Hymenocallis littoralis) in Malaysia |
| title_sort | identification and characterization of macrophomina phaseolina causing leaf blight on white spider lilies crinum asiaticum and hymenocallis littoralis in malaysia |
| topic | macrophomina phaseolina leaf blight crinum asiaticum hymenocallis littoralis |
| url | http://dx.doi.org/10.1080/12298093.2019.1682448 |
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