Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange system
Pacific white shrimp (Litopenaeus vannamei) were grown in a biofloc rearing systems with (control treatment) or without sugarcane bagasse (SB treatment), with 3 replicate systems per treatment. SB, is an economic carbohydrate source. Parameters monitored were water-quality, growth indices, digestive...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513423003277 |
| _version_ | 1797388850190876672 |
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| author | Seham A.H. Hassan Zaki Z. Sharawy Shabaan A. Hemeda Abeer F. El Nahas Eman M. Abbas Hala Saber Khalil Marc Verdegem |
| author_facet | Seham A.H. Hassan Zaki Z. Sharawy Shabaan A. Hemeda Abeer F. El Nahas Eman M. Abbas Hala Saber Khalil Marc Verdegem |
| author_sort | Seham A.H. Hassan |
| collection | DOAJ |
| description | Pacific white shrimp (Litopenaeus vannamei) were grown in a biofloc rearing systems with (control treatment) or without sugarcane bagasse (SB treatment), with 3 replicate systems per treatment. SB, is an economic carbohydrate source. Parameters monitored were water-quality, growth indices, digestive enzymes activity, and microbial dynamics. Messenger RNA levels of immune-related, growth-related, and antioxidant genes in hepatopancreas and muscle and gene's single nucleotide polymorphism (SNP) were also considered. The outdoor ponds (44.5 m3), were each stocked with 600 L. vannamei larvae with an initial weight of 0.024 g) to start a three-month experiment. The biofloc development was in two aerated active suspension tanks to which SB (about 700 g/day, adjusted every two weeks) was added to maintain the of the C/N at 16. The water quality parameters, digestive enzyme activity, growth, and survival (P ≤ 0.05) in the SB group was improved compared to the control. SB also increased the total heterotrophic microbiota compared to the control. The mRNA levels of immune- related genes (lipopolysaccharides/b-glucan- binding protein, β-glucan-binding protein, lysozyme, prophenoloxidase, crustin, transglutaminase, and hemocyanin) and antioxidant genes (superoxide dismutase and glutathione peroxidase) significantly increased in SB group (P ≤ 0.05) compared to the control treatment. The SB group was the highest in all growth- related genes such as insulin- like growth factors (I and II), growth hormone, myosin, and paramyosin. Eight SNPs were detected in the hemocyanin gene, and an adenine insertion (ins A-554) was recorded at a high body weight. Our findings implied that using SB, as a carbon source in biofloc system without artificial feeding, could improve water quality, growth indices, digestive enzymes, microbial dynamics and the mRNA levels of all immune-, growth- related and antioxidant genes in L. vannamei. |
| first_indexed | 2024-03-08T22:46:47Z |
| format | Article |
| id | doaj.art-ef7697ecdfb740d5938815aafc68c648 |
| institution | Directory Open Access Journal |
| issn | 2352-5134 |
| language | English |
| last_indexed | 2024-03-08T22:46:47Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj.art-ef7697ecdfb740d5938815aafc68c6482023-12-17T06:39:43ZengElsevierAquaculture Reports2352-51342023-12-0133101788Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange systemSeham A.H. Hassan0Zaki Z. Sharawy1Shabaan A. Hemeda2Abeer F. El Nahas3Eman M. Abbas4Hala Saber Khalil5Marc Verdegem6National Institute of Oceanography and Fisheries, Aquaculture Division, NIOF, Egypt; Department of Animal Husbandry and Animal Wealth Development. Faculty of Veterinary Medicine, Alexandria University, EgyptNational Institute of Oceanography and Fisheries, Aquaculture Division, NIOF, EgyptDepartment of Animal Husbandry and Animal Wealth Development. Faculty of Veterinary Medicine, Alexandria University, EgyptDepartment of Animal Husbandry and Animal Wealth Development. Faculty of Veterinary Medicine, Alexandria University, EgyptNational Institute of Oceanography and Fisheries, Aquaculture Division, NIOF, EgyptAquaculture Department, Faculty of Fish Resources, Suez University, Suez, Egypt; College of Fisheries and Aquaculture Technology, Arab Academy for Science, Technology, and Maritime Transport, Alexandria, Egypt; Corresponding author at: Aquaculture Department, Faculty of Fish Resources, Suez University, Suez, Egypt.Wageningen Institute of Animal Sciences, Aquaculture and Fisheries Group, Wageningen University and Research, Wageningen, the Netherlands; Corresponding author.Pacific white shrimp (Litopenaeus vannamei) were grown in a biofloc rearing systems with (control treatment) or without sugarcane bagasse (SB treatment), with 3 replicate systems per treatment. SB, is an economic carbohydrate source. Parameters monitored were water-quality, growth indices, digestive enzymes activity, and microbial dynamics. Messenger RNA levels of immune-related, growth-related, and antioxidant genes in hepatopancreas and muscle and gene's single nucleotide polymorphism (SNP) were also considered. The outdoor ponds (44.5 m3), were each stocked with 600 L. vannamei larvae with an initial weight of 0.024 g) to start a three-month experiment. The biofloc development was in two aerated active suspension tanks to which SB (about 700 g/day, adjusted every two weeks) was added to maintain the of the C/N at 16. The water quality parameters, digestive enzyme activity, growth, and survival (P ≤ 0.05) in the SB group was improved compared to the control. SB also increased the total heterotrophic microbiota compared to the control. The mRNA levels of immune- related genes (lipopolysaccharides/b-glucan- binding protein, β-glucan-binding protein, lysozyme, prophenoloxidase, crustin, transglutaminase, and hemocyanin) and antioxidant genes (superoxide dismutase and glutathione peroxidase) significantly increased in SB group (P ≤ 0.05) compared to the control treatment. The SB group was the highest in all growth- related genes such as insulin- like growth factors (I and II), growth hormone, myosin, and paramyosin. Eight SNPs were detected in the hemocyanin gene, and an adenine insertion (ins A-554) was recorded at a high body weight. Our findings implied that using SB, as a carbon source in biofloc system without artificial feeding, could improve water quality, growth indices, digestive enzymes, microbial dynamics and the mRNA levels of all immune-, growth- related and antioxidant genes in L. vannamei.http://www.sciencedirect.com/science/article/pii/S2352513423003277Litopenaeus vannameiBioflocSugarcane bagasseDigestive enzymesGenes expression |
| spellingShingle | Seham A.H. Hassan Zaki Z. Sharawy Shabaan A. Hemeda Abeer F. El Nahas Eman M. Abbas Hala Saber Khalil Marc Verdegem Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange system Aquaculture Reports Litopenaeus vannamei Biofloc Sugarcane bagasse Digestive enzymes Genes expression |
| title | Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange system |
| title_full | Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange system |
| title_fullStr | Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange system |
| title_full_unstemmed | Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange system |
| title_short | Sugarcane bagasse improved growth performance, digestive enzyme activity, microbial dynamics, and mRNA transcripts of immune-, growth, and antioxidant -related genes of Litopenaeus vannamei in a zero-water exchange system |
| title_sort | sugarcane bagasse improved growth performance digestive enzyme activity microbial dynamics and mrna transcripts of immune growth and antioxidant related genes of litopenaeus vannamei in a zero water exchange system |
| topic | Litopenaeus vannamei Biofloc Sugarcane bagasse Digestive enzymes Genes expression |
| url | http://www.sciencedirect.com/science/article/pii/S2352513423003277 |
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