NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway
Abstract Newcastle disease (ND), caused by the Newcastle disease virus (NDV), is a highly virulent infectious disease of poultry. Virulent NDV can cause severe autophagy and inflammation in host cells. While studies have shown a mutual regulatory relationship between autophagy and inflammation, this...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-06-01
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| Series: | Veterinary Research |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13567-023-01174-w |
| _version_ | 1827928526657945600 |
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| author | Juncheng Cai Siyuan Wang Haoyun Du Lei Fan WeiFeng Yuan Qiufan Xu Jinlian Ren Qiuyan Lin Bin Xiang Chan Ding Tao Ren Libin Chen |
| author_facet | Juncheng Cai Siyuan Wang Haoyun Du Lei Fan WeiFeng Yuan Qiufan Xu Jinlian Ren Qiuyan Lin Bin Xiang Chan Ding Tao Ren Libin Chen |
| author_sort | Juncheng Cai |
| collection | DOAJ |
| description | Abstract Newcastle disease (ND), caused by the Newcastle disease virus (NDV), is a highly virulent infectious disease of poultry. Virulent NDV can cause severe autophagy and inflammation in host cells. While studies have shown a mutual regulatory relationship between autophagy and inflammation, this relationship in NDV infection remains unclear. This study confirmed that NDV infection could trigger autophagy in DF-1 cells to promote cytopathic and viral replication. NDV-induced autophagy was positively correlated with the mRNA levels of inflammatory cytokines such as IL-1β, IL-8, IL-18, CCL-5, and TNF-α, suggesting that NDV-induced autophagy promotes the expression of inflammatory cytokines. Further investigation demonstrated that NLRP3 protein expression, Caspase-1 activity, and p38 phosphorylation level positively correlated with autophagy, suggesting that NDV-induced autophagy could promote the expression of inflammatory cytokines through NLRP3/Caspase-1 inflammasomes and p38/MAPK pathway. In addition, NDV infection also triggered mitochondrial damage and mitophagy in DF-1 cells, but did not result in a large leakage of reactive oxygen species (ROS) and mitochondrial DNA (mtDNA), indicating that mitochondrial damage and mitophagy do not contribute to the inflammation response during NDV infection. |
| first_indexed | 2024-03-13T06:09:57Z |
| format | Article |
| id | doaj.art-ef7879618c05495ead8e076d47bb950e |
| institution | Directory Open Access Journal |
| issn | 1297-9716 |
| language | English |
| last_indexed | 2024-03-13T06:09:57Z |
| publishDate | 2023-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Veterinary Research |
| spelling | doaj.art-ef7879618c05495ead8e076d47bb950e2023-06-11T11:22:31ZengBMCVeterinary Research1297-97162023-06-0154111510.1186/s13567-023-01174-wNDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathwayJuncheng Cai0Siyuan Wang1Haoyun Du2Lei Fan3WeiFeng Yuan4Qiufan Xu5Jinlian Ren6Qiuyan Lin7Bin Xiang8Chan Ding9Tao Ren10Libin Chen11College of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, Yunnan Agricultural UniversityShanghai Veterinary Research Institute, Chinese Academy of Agricultural SciencesCollege of Veterinary Medicine, South China Agricultural UniversityCollege of Veterinary Medicine, South China Agricultural UniversityAbstract Newcastle disease (ND), caused by the Newcastle disease virus (NDV), is a highly virulent infectious disease of poultry. Virulent NDV can cause severe autophagy and inflammation in host cells. While studies have shown a mutual regulatory relationship between autophagy and inflammation, this relationship in NDV infection remains unclear. This study confirmed that NDV infection could trigger autophagy in DF-1 cells to promote cytopathic and viral replication. NDV-induced autophagy was positively correlated with the mRNA levels of inflammatory cytokines such as IL-1β, IL-8, IL-18, CCL-5, and TNF-α, suggesting that NDV-induced autophagy promotes the expression of inflammatory cytokines. Further investigation demonstrated that NLRP3 protein expression, Caspase-1 activity, and p38 phosphorylation level positively correlated with autophagy, suggesting that NDV-induced autophagy could promote the expression of inflammatory cytokines through NLRP3/Caspase-1 inflammasomes and p38/MAPK pathway. In addition, NDV infection also triggered mitochondrial damage and mitophagy in DF-1 cells, but did not result in a large leakage of reactive oxygen species (ROS) and mitochondrial DNA (mtDNA), indicating that mitochondrial damage and mitophagy do not contribute to the inflammation response during NDV infection.https://doi.org/10.1186/s13567-023-01174-wAutophagyinflammationmitochondrial damagemitophagyNewcastle disease virus |
| spellingShingle | Juncheng Cai Siyuan Wang Haoyun Du Lei Fan WeiFeng Yuan Qiufan Xu Jinlian Ren Qiuyan Lin Bin Xiang Chan Ding Tao Ren Libin Chen NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway Veterinary Research Autophagy inflammation mitochondrial damage mitophagy Newcastle disease virus |
| title | NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway |
| title_full | NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway |
| title_fullStr | NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway |
| title_full_unstemmed | NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway |
| title_short | NDV-induced autophagy enhances inflammation through NLRP3/Caspase-1 inflammasomes and the p38/MAPK pathway |
| title_sort | ndv induced autophagy enhances inflammation through nlrp3 caspase 1 inflammasomes and the p38 mapk pathway |
| topic | Autophagy inflammation mitochondrial damage mitophagy Newcastle disease virus |
| url | https://doi.org/10.1186/s13567-023-01174-w |
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