The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) Seeds
The addition of nanoparticles has been reported to be an effective strategy for enhancing seed germination, but the underlying mechanisms whereby this occurs are unclear. In the present study, we added silica nanoparticles (SiNPs) to an aqueous growth medium in which tomato seeds were germinated. We...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-07-01
|
| Series: | Nanomaterials |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-4991/13/14/2110 |
| _version_ | 1827732211607011328 |
|---|---|
| author | Anca Awal Sembada Shinya Maki Ahmad Faizal Toshiyuki Fukuhara Takeshi Suzuki I. Wuled Lenggoro |
| author_facet | Anca Awal Sembada Shinya Maki Ahmad Faizal Toshiyuki Fukuhara Takeshi Suzuki I. Wuled Lenggoro |
| author_sort | Anca Awal Sembada |
| collection | DOAJ |
| description | The addition of nanoparticles has been reported to be an effective strategy for enhancing seed germination, but the underlying mechanisms whereby this occurs are unclear. In the present study, we added silica nanoparticles (SiNPs) to an aqueous growth medium in which tomato seeds were germinated. We examined the effects of SiNPs on growth and possible mechanisms of action. SiNPs had a diameter of 10–17 nm and 110–120 nm. SiNPs shortened the mean germination time from 5.24 ± 0.29 days to 4.64 ± 0.29 days. Seedling vigor, measured by criteria including length and weight, was also improved compared to the control condition. The presence of SiNPs in the seedlings was assessed using an X-ray fluorescence spectrometer. The nanoparticles may have promoted germination by enhancing water imbibition by the seeds or altering the external microenvironment. Scanning electron microscopy revealed changes in the seed coat during germination, many of which were only observed in the presence of nanoparticles. Soil bacteria affect germination; specifically, <i>Bacillus</i> sp. may promote germination. The number of <i>Bacillus</i> sp. changed in the germination medium with SiNPs compared to the control. This suggested that these bacteria could interact with SiNPs to promote germination. |
| first_indexed | 2024-03-11T00:46:28Z |
| format | Article |
| id | doaj.art-ef7ae1cc37c349cda70e528477f84a59 |
| institution | Directory Open Access Journal |
| issn | 2079-4991 |
| language | English |
| last_indexed | 2024-03-11T00:46:28Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nanomaterials |
| spelling | doaj.art-ef7ae1cc37c349cda70e528477f84a592023-11-18T20:46:08ZengMDPI AGNanomaterials2079-49912023-07-011314211010.3390/nano13142110The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) SeedsAnca Awal Sembada0Shinya Maki1Ahmad Faizal2Toshiyuki Fukuhara3Takeshi Suzuki4I. Wuled Lenggoro5Chemical Engineering Program, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Koganei 184-8588, Tokyo, JapanDepartment of Science of Technology Innovation, Nagaoka University of Technology, Nagaoka 940-2188, Niigata, JapanPlant Science and Biotechnology Research Group, School of Life Sciences and Technology, Institut Teknologi Bandung, Bandung 40132, IndonesiaDepartment of Applied Biological Science, Graduate School of Agriculture, Tokyo University of Agriculture and Technology, Fuchu 183-8509, Tokyo, JapanGraduate School of Bio-Applications & Systems Engineering, Tokyo University of Agriculture and Technology, Koganei 184-8588, Tokyo, JapanChemical Engineering Program, Graduate School of Engineering, Tokyo University of Agriculture and Technology, Koganei 184-8588, Tokyo, JapanThe addition of nanoparticles has been reported to be an effective strategy for enhancing seed germination, but the underlying mechanisms whereby this occurs are unclear. In the present study, we added silica nanoparticles (SiNPs) to an aqueous growth medium in which tomato seeds were germinated. We examined the effects of SiNPs on growth and possible mechanisms of action. SiNPs had a diameter of 10–17 nm and 110–120 nm. SiNPs shortened the mean germination time from 5.24 ± 0.29 days to 4.64 ± 0.29 days. Seedling vigor, measured by criteria including length and weight, was also improved compared to the control condition. The presence of SiNPs in the seedlings was assessed using an X-ray fluorescence spectrometer. The nanoparticles may have promoted germination by enhancing water imbibition by the seeds or altering the external microenvironment. Scanning electron microscopy revealed changes in the seed coat during germination, many of which were only observed in the presence of nanoparticles. Soil bacteria affect germination; specifically, <i>Bacillus</i> sp. may promote germination. The number of <i>Bacillus</i> sp. changed in the germination medium with SiNPs compared to the control. This suggested that these bacteria could interact with SiNPs to promote germination.https://www.mdpi.com/2079-4991/13/14/2110silicon dioxidecolloidplantsgrowth<i>Bacillus</i> sp. |
| spellingShingle | Anca Awal Sembada Shinya Maki Ahmad Faizal Toshiyuki Fukuhara Takeshi Suzuki I. Wuled Lenggoro The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) Seeds Nanomaterials silicon dioxide colloid plants growth <i>Bacillus</i> sp. |
| title | The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) Seeds |
| title_full | The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) Seeds |
| title_fullStr | The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) Seeds |
| title_full_unstemmed | The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) Seeds |
| title_short | The Role of Silica Nanoparticles in Promoting the Germination of Tomato (<i>Solanum lycopersicum</i>) Seeds |
| title_sort | role of silica nanoparticles in promoting the germination of tomato i solanum lycopersicum i seeds |
| topic | silicon dioxide colloid plants growth <i>Bacillus</i> sp. |
| url | https://www.mdpi.com/2079-4991/13/14/2110 |
| work_keys_str_mv | AT ancaawalsembada theroleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT shinyamaki theroleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT ahmadfaizal theroleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT toshiyukifukuhara theroleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT takeshisuzuki theroleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT iwuledlenggoro theroleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT ancaawalsembada roleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT shinyamaki roleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT ahmadfaizal roleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT toshiyukifukuhara roleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT takeshisuzuki roleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds AT iwuledlenggoro roleofsilicananoparticlesinpromotingthegerminationoftomatoisolanumlycopersicumiseeds |