Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand
Leptospirosis is a neglected zoonotic disease affecting mostly the world’s tropical regions. The rural people of northeastern Thailand suffer from a large number of leptospirosis infections, and their abundant rice fields are optimal rodent habitats. To evaluate the contribution of diversity and car...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2020-09-01
|
| Series: | Tropical Medicine and Infectious Disease |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2414-6366/5/4/154 |
| _version_ | 1797552184545509376 |
|---|---|
| author | Panadda Krairojananan Janjira Thaipadungpanit Surachai Leepitakrat Taweesak Monkanna Elizabeth W. Wanja Anthony L. Schuster Federico Costa B. Katherine Poole-Smith Patrick W. McCardle |
| author_facet | Panadda Krairojananan Janjira Thaipadungpanit Surachai Leepitakrat Taweesak Monkanna Elizabeth W. Wanja Anthony L. Schuster Federico Costa B. Katherine Poole-Smith Patrick W. McCardle |
| author_sort | Panadda Krairojananan |
| collection | DOAJ |
| description | Leptospirosis is a neglected zoonotic disease affecting mostly the world’s tropical regions. The rural people of northeastern Thailand suffer from a large number of leptospirosis infections, and their abundant rice fields are optimal rodent habitats. To evaluate the contribution of diversity and carriage rate of pathogenic <i>Leptospira</i> in rodent reservoirs to leptospirosis incidence, we surveyed rodents, between 2011 and 2012, in four provinces in northeastern Thailand with the highest incidence rates of human leptospirosis cases. We used <i>lipL32</i> real-time PCR to detect pathogenic <i>Leptospira</i> in rodent kidneys, partial 16S rRNA gene sequencing to classify the infecting <i>Leptospira</i> species, and whole 16S rDNA sequencing to classify species of isolated <i>Leptospira</i>. Overall prevalence of <i>Leptospira</i> infection was 3.6% (18/495). Among infected rodents, <i>Bandicota</i><i>indica</i> (14.3%), <i>Rattus</i><i>exulans</i> (3.6%), and <i>R</i>. <i>rattus</i> (3.2%) had renal carriage. We identified two pathogenic <i>Leptospira</i> species: <i>L</i>. <i>interrogans</i> (<i>n</i> = 15) and <i>L</i>. <i>borgpetersenii</i> (<i>n</i> = 3). In addition, an <i>L</i>. <i>wolffii</i> (LS0914U) isolate was recovered from the urine of <i>B</i>. <i>indica</i>. <i>Leptospira</i> infection was more prevalent in low density rodent populations, such as <i>B</i>. <i>indica</i>. In contrast, there was a lower prevalence of <i>Leptospira</i> infection in high density rodent populations of <i>R</i>. <i>exulans</i> and <i>R</i>. <i>rattus</i>. |
| first_indexed | 2024-03-10T15:56:23Z |
| format | Article |
| id | doaj.art-ef7d57d27dce4287bb15205c0d3f815d |
| institution | Directory Open Access Journal |
| issn | 2414-6366 |
| language | English |
| last_indexed | 2024-03-10T15:56:23Z |
| publishDate | 2020-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Tropical Medicine and Infectious Disease |
| spelling | doaj.art-ef7d57d27dce4287bb15205c0d3f815d2023-11-20T15:41:46ZengMDPI AGTropical Medicine and Infectious Disease2414-63662020-09-015415410.3390/tropicalmed5040154Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern ThailandPanadda Krairojananan0Janjira Thaipadungpanit1Surachai Leepitakrat2Taweesak Monkanna3Elizabeth W. Wanja4Anthony L. Schuster5Federico Costa6B. Katherine Poole-Smith7Patrick W. McCardle8Department of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandDepartment of Clinical Tropical Medicine, Faculty of Tropical Medicine, Mahidol University, Bangkok 10400, ThailandDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandDepartment of Preventive Medicine and Biostatistics, Uniformed Services University of the Health Sciences, 4301 Jones Bridge Road, Bethesda, MD 20814, USAMEDCOM Headquarters, Fort Sam Houston, San Antonio, TX 78234, USAThe Institute of Collective Health (ISC), Federal University of Bahia (UFBA), Rua Basílio da Gama, 316, Canela, Salvador CEP 40110-040, BrazilDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandDepartment of Entomology, Armed Forces Research Institute of Medical Sciences (AFRIMS), Bangkok 10400, ThailandLeptospirosis is a neglected zoonotic disease affecting mostly the world’s tropical regions. The rural people of northeastern Thailand suffer from a large number of leptospirosis infections, and their abundant rice fields are optimal rodent habitats. To evaluate the contribution of diversity and carriage rate of pathogenic <i>Leptospira</i> in rodent reservoirs to leptospirosis incidence, we surveyed rodents, between 2011 and 2012, in four provinces in northeastern Thailand with the highest incidence rates of human leptospirosis cases. We used <i>lipL32</i> real-time PCR to detect pathogenic <i>Leptospira</i> in rodent kidneys, partial 16S rRNA gene sequencing to classify the infecting <i>Leptospira</i> species, and whole 16S rDNA sequencing to classify species of isolated <i>Leptospira</i>. Overall prevalence of <i>Leptospira</i> infection was 3.6% (18/495). Among infected rodents, <i>Bandicota</i><i>indica</i> (14.3%), <i>Rattus</i><i>exulans</i> (3.6%), and <i>R</i>. <i>rattus</i> (3.2%) had renal carriage. We identified two pathogenic <i>Leptospira</i> species: <i>L</i>. <i>interrogans</i> (<i>n</i> = 15) and <i>L</i>. <i>borgpetersenii</i> (<i>n</i> = 3). In addition, an <i>L</i>. <i>wolffii</i> (LS0914U) isolate was recovered from the urine of <i>B</i>. <i>indica</i>. <i>Leptospira</i> infection was more prevalent in low density rodent populations, such as <i>B</i>. <i>indica</i>. In contrast, there was a lower prevalence of <i>Leptospira</i> infection in high density rodent populations of <i>R</i>. <i>exulans</i> and <i>R</i>. <i>rattus</i>.https://www.mdpi.com/2414-6366/5/4/154carriage<i>Leptospira</i><i>L</i>. <i>wolffii</i>rodent<i>Bandicota</i> <i>indica</i>16S rRNA gene |
| spellingShingle | Panadda Krairojananan Janjira Thaipadungpanit Surachai Leepitakrat Taweesak Monkanna Elizabeth W. Wanja Anthony L. Schuster Federico Costa B. Katherine Poole-Smith Patrick W. McCardle Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand Tropical Medicine and Infectious Disease carriage <i>Leptospira</i> <i>L</i>. <i>wolffii</i> rodent <i>Bandicota</i> <i>indica</i> 16S rRNA gene |
| title | Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand |
| title_full | Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand |
| title_fullStr | Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand |
| title_full_unstemmed | Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand |
| title_short | Low Prevalence of <i>Leptospira</i> Carriage in Rodents in Leptospirosis-Endemic Northeastern Thailand |
| title_sort | low prevalence of i leptospira i carriage in rodents in leptospirosis endemic northeastern thailand |
| topic | carriage <i>Leptospira</i> <i>L</i>. <i>wolffii</i> rodent <i>Bandicota</i> <i>indica</i> 16S rRNA gene |
| url | https://www.mdpi.com/2414-6366/5/4/154 |
| work_keys_str_mv | AT panaddakrairojananan lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT janjirathaipadungpanit lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT surachaileepitakrat lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT taweesakmonkanna lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT elizabethwwanja lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT anthonylschuster lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT federicocosta lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT bkatherinepoolesmith lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand AT patrickwmccardle lowprevalenceofileptospiraicarriageinrodentsinleptospirosisendemicnortheasternthailand |