Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell line
Rasfonin (A304) is a fungal natural product isolated from the fermentation substrate of Talaromyces sp. 3656-A1, which was named according to its activity against the small G-protein Ras. In a former study, we demonstrated that it induced autophagy and apoptosis; however, whether rasfonin activated...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2016-04-01
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| Series: | Mycology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/21501203.2016.1181114 |
| _version_ | 1819014632177664000 |
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| author | Hui Sun Weijun Wang Yongsheng Che Xuejun Jiang |
| author_facet | Hui Sun Weijun Wang Yongsheng Che Xuejun Jiang |
| author_sort | Hui Sun |
| collection | DOAJ |
| description | Rasfonin (A304) is a fungal natural product isolated from the fermentation substrate of Talaromyces sp. 3656-A1, which was named according to its activity against the small G-protein Ras. In a former study, we demonstrated that it induced autophagy and apoptosis; however, whether rasfonin activated necroptosis remained unknown. Moreover, the interplay among different cell death processes induced by rasfonin was unexplored. In the present study, we revealed that, in addition of promoting autophagy and caspase-dependent apoptosis, rasfonin also activated necroptosis. Nectrostatin-1 (Nec-1), an inhibitor of necroptosis, affected rasfonin-induced autophagy in a time-dependent manner concurring with an increased caspase-dependent apoptosis. The aforementioned results were confirmed by knockdown of receptor-interacting protein 1 (RIP1), a crucial necrostatin-1-targeted adaptor kinase mediating cell death and survival. Taken together, the data presented indicate that rasfonin activates various cell death pathways, and RIP1 plays a critical role in rasfonin-induced autophagy and apoptosis. |
| first_indexed | 2024-12-21T02:18:55Z |
| format | Article |
| id | doaj.art-ef81d6fa34044ef7a2b5b1138de3a878 |
| institution | Directory Open Access Journal |
| issn | 2150-1203 2150-1211 |
| language | English |
| last_indexed | 2024-12-21T02:18:55Z |
| publishDate | 2016-04-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Mycology |
| spelling | doaj.art-ef81d6fa34044ef7a2b5b1138de3a8782022-12-21T19:19:13ZengTaylor & Francis GroupMycology2150-12032150-12112016-04-0172818710.1080/21501203.2016.11811141181114Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell lineHui Sun0Weijun Wang1Yongsheng Che2Xuejun Jiang3Chinese Academy of SciencesChinese Academy of SciencesBeijing Institute of Pharmacology & ToxicologyChinese Academy of SciencesRasfonin (A304) is a fungal natural product isolated from the fermentation substrate of Talaromyces sp. 3656-A1, which was named according to its activity against the small G-protein Ras. In a former study, we demonstrated that it induced autophagy and apoptosis; however, whether rasfonin activated necroptosis remained unknown. Moreover, the interplay among different cell death processes induced by rasfonin was unexplored. In the present study, we revealed that, in addition of promoting autophagy and caspase-dependent apoptosis, rasfonin also activated necroptosis. Nectrostatin-1 (Nec-1), an inhibitor of necroptosis, affected rasfonin-induced autophagy in a time-dependent manner concurring with an increased caspase-dependent apoptosis. The aforementioned results were confirmed by knockdown of receptor-interacting protein 1 (RIP1), a crucial necrostatin-1-targeted adaptor kinase mediating cell death and survival. Taken together, the data presented indicate that rasfonin activates various cell death pathways, and RIP1 plays a critical role in rasfonin-induced autophagy and apoptosis.http://dx.doi.org/10.1080/21501203.2016.1181114RasfoninautophagyapoptosisnecroptosisRIP1 |
| spellingShingle | Hui Sun Weijun Wang Yongsheng Che Xuejun Jiang Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell line Mycology Rasfonin autophagy apoptosis necroptosis RIP1 |
| title | Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell line |
| title_full | Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell line |
| title_fullStr | Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell line |
| title_full_unstemmed | Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell line |
| title_short | Fungal secondary metabolites rasfonin induces autophagy, apoptosis and necroptosis in renal cancer cell line |
| title_sort | fungal secondary metabolites rasfonin induces autophagy apoptosis and necroptosis in renal cancer cell line |
| topic | Rasfonin autophagy apoptosis necroptosis RIP1 |
| url | http://dx.doi.org/10.1080/21501203.2016.1181114 |
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