Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i>
High molecular weight glutenin subunits (HMW-GSs) play a major role in determining the dough quality of wheat. As the D genome donor of hexaploid wheat, <i>Aegilops tauschii</i> is an important genetic resource for wheat quality breeding. In the present study, a novel HMW-GSs from <i&...
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MDPI AG
2022-11-01
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| Series: | Agronomy |
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| Online Access: | https://www.mdpi.com/2073-4395/12/11/2709 |
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| author | Cunyao Bo Zhongqing Fan Xin Ma Anfei Li Hongwei Wang Lingrang Kong Xiaoqian Wang |
| author_facet | Cunyao Bo Zhongqing Fan Xin Ma Anfei Li Hongwei Wang Lingrang Kong Xiaoqian Wang |
| author_sort | Cunyao Bo |
| collection | DOAJ |
| description | High molecular weight glutenin subunits (HMW-GSs) play a major role in determining the dough quality of wheat. As the D genome donor of hexaploid wheat, <i>Aegilops tauschii</i> is an important genetic resource for wheat quality breeding. In the present study, a novel HMW-GSs from <i>Ae</i><i>. tauschii</i> was identified and designated as <i>Glu-D<sup>t</sup>1</i>. Multiple sequence alignment indicated that one cysteine was mutated into arginine in the y-type subunit. Site-directed mutagenesis technology was applied to verify the function of gene <i>Glu-D<sup>t</sup>1</i>. Three introgression lines (ILs), B9, B25, and B35 with the <i>Glu-D1</i> loci substituted by <i>Glu-D<sup>t</sup>1</i> were detected from the BC<sub>3</sub>F<sub>5</sub> population derived from hexaploid wheat cultivar Jimai22 and <i>Ae</i><i>. tauschii</i> Y215 through the direct hybridization approach. The dough quality and agronomic performance analysis were performed, which provide valuable resources for wheat genetic studies and breeding for distinctive end-use quality. |
| first_indexed | 2024-03-09T19:21:31Z |
| format | Article |
| id | doaj.art-ef82f7f2d0a64c158497c664f8736f51 |
| institution | Directory Open Access Journal |
| issn | 2073-4395 |
| language | English |
| last_indexed | 2024-03-09T19:21:31Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Agronomy |
| spelling | doaj.art-ef82f7f2d0a64c158497c664f8736f512023-11-24T03:20:57ZengMDPI AGAgronomy2073-43952022-11-011211270910.3390/agronomy12112709Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i>Cunyao Bo0Zhongqing Fan1Xin Ma2Anfei Li3Hongwei Wang4Lingrang Kong5Xiaoqian Wang6State Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaShandong Nongda Fertilizer Sci & Tech Co., Ltd., Tai’an 271000, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaState Key Laboratory of Crop Biology, College of Agronomy, Shandong Agricultural University, Tai’an 271018, ChinaHigh molecular weight glutenin subunits (HMW-GSs) play a major role in determining the dough quality of wheat. As the D genome donor of hexaploid wheat, <i>Aegilops tauschii</i> is an important genetic resource for wheat quality breeding. In the present study, a novel HMW-GSs from <i>Ae</i><i>. tauschii</i> was identified and designated as <i>Glu-D<sup>t</sup>1</i>. Multiple sequence alignment indicated that one cysteine was mutated into arginine in the y-type subunit. Site-directed mutagenesis technology was applied to verify the function of gene <i>Glu-D<sup>t</sup>1</i>. Three introgression lines (ILs), B9, B25, and B35 with the <i>Glu-D1</i> loci substituted by <i>Glu-D<sup>t</sup>1</i> were detected from the BC<sub>3</sub>F<sub>5</sub> population derived from hexaploid wheat cultivar Jimai22 and <i>Ae</i><i>. tauschii</i> Y215 through the direct hybridization approach. The dough quality and agronomic performance analysis were performed, which provide valuable resources for wheat genetic studies and breeding for distinctive end-use quality.https://www.mdpi.com/2073-4395/12/11/2709wheat<i>Aegilops tauschii</i>HMW-GSsintrogression linesdough quality |
| spellingShingle | Cunyao Bo Zhongqing Fan Xin Ma Anfei Li Hongwei Wang Lingrang Kong Xiaoqian Wang Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i> Agronomy wheat <i>Aegilops tauschii</i> HMW-GSs introgression lines dough quality |
| title | Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i> |
| title_full | Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i> |
| title_fullStr | Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i> |
| title_full_unstemmed | Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i> |
| title_short | Identification and Introgression of a Novel HMW-GS Gene from <i>Aegilops tauschii</i> |
| title_sort | identification and introgression of a novel hmw gs gene from i aegilops tauschii i |
| topic | wheat <i>Aegilops tauschii</i> HMW-GSs introgression lines dough quality |
| url | https://www.mdpi.com/2073-4395/12/11/2709 |
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