Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions.
For adaptation between anaerobic, micro-aerobic and aerobic conditions Escherichia coli's metabolism and in particular its electron transport chain (ETC) is highly regulated. Although it is known that the global transcriptional regulators FNR and ArcA are involved in oxygen response it is uncle...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4182436?pdf=render |
| _version_ | 1818618258499043328 |
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| author | Sebastian G Henkel Alexander Ter Beek Sonja Steinsiek Stefan Stagge Katja Bettenbrock M Joost Teixeira de Mattos Thomas Sauter Oliver Sawodny Michael Ederer |
| author_facet | Sebastian G Henkel Alexander Ter Beek Sonja Steinsiek Stefan Stagge Katja Bettenbrock M Joost Teixeira de Mattos Thomas Sauter Oliver Sawodny Michael Ederer |
| author_sort | Sebastian G Henkel |
| collection | DOAJ |
| description | For adaptation between anaerobic, micro-aerobic and aerobic conditions Escherichia coli's metabolism and in particular its electron transport chain (ETC) is highly regulated. Although it is known that the global transcriptional regulators FNR and ArcA are involved in oxygen response it is unclear how they interplay in the regulation of ETC enzymes under micro-aerobic chemostat conditions. Also, there are diverse results which and how quinones (oxidised/reduced, ubiquinone/other quinones) are controlling the ArcBA two-component system. In the following a mathematical model of the E. coli ETC linked to basic modules for substrate uptake, fermentation product excretion and biomass formation is introduced. The kinetic modelling focusses on regulatory principles of the ETC for varying oxygen conditions in glucose-limited continuous cultures. The model is based on the balance of electron donation (glucose) and acceptance (oxygen or other acceptors). Also, it is able to account for different chemostat conditions due to changed substrate concentrations and dilution rates. The parameter identification process is divided into an estimation and a validation step based on previously published and new experimental data. The model shows that experimentally observed, qualitatively different behaviour of the ubiquinone redox state and the ArcA activity profile in the micro-aerobic range for different experimental conditions can emerge from a single network structure. The network structure features a strong feed-forward effect from the FNR regulatory system to the ArcBA regulatory system via a common control of the dehydrogenases of the ETC. The model supports the hypothesis that ubiquinone but not ubiquinol plays a key role in determining the activity of ArcBA in a glucose-limited chemostat at micro-aerobic conditions. |
| first_indexed | 2024-12-16T17:18:44Z |
| format | Article |
| id | doaj.art-ef8407dfb30f4733b0841ff93157e077 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-16T17:18:44Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ef8407dfb30f4733b0841ff93157e0772022-12-21T22:23:13ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10764010.1371/journal.pone.0107640Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions.Sebastian G HenkelAlexander Ter BeekSonja SteinsiekStefan StaggeKatja BettenbrockM Joost Teixeira de MattosThomas SauterOliver SawodnyMichael EdererFor adaptation between anaerobic, micro-aerobic and aerobic conditions Escherichia coli's metabolism and in particular its electron transport chain (ETC) is highly regulated. Although it is known that the global transcriptional regulators FNR and ArcA are involved in oxygen response it is unclear how they interplay in the regulation of ETC enzymes under micro-aerobic chemostat conditions. Also, there are diverse results which and how quinones (oxidised/reduced, ubiquinone/other quinones) are controlling the ArcBA two-component system. In the following a mathematical model of the E. coli ETC linked to basic modules for substrate uptake, fermentation product excretion and biomass formation is introduced. The kinetic modelling focusses on regulatory principles of the ETC for varying oxygen conditions in glucose-limited continuous cultures. The model is based on the balance of electron donation (glucose) and acceptance (oxygen or other acceptors). Also, it is able to account for different chemostat conditions due to changed substrate concentrations and dilution rates. The parameter identification process is divided into an estimation and a validation step based on previously published and new experimental data. The model shows that experimentally observed, qualitatively different behaviour of the ubiquinone redox state and the ArcA activity profile in the micro-aerobic range for different experimental conditions can emerge from a single network structure. The network structure features a strong feed-forward effect from the FNR regulatory system to the ArcBA regulatory system via a common control of the dehydrogenases of the ETC. The model supports the hypothesis that ubiquinone but not ubiquinol plays a key role in determining the activity of ArcBA in a glucose-limited chemostat at micro-aerobic conditions.http://europepmc.org/articles/PMC4182436?pdf=render |
| spellingShingle | Sebastian G Henkel Alexander Ter Beek Sonja Steinsiek Stefan Stagge Katja Bettenbrock M Joost Teixeira de Mattos Thomas Sauter Oliver Sawodny Michael Ederer Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions. PLoS ONE |
| title | Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions. |
| title_full | Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions. |
| title_fullStr | Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions. |
| title_full_unstemmed | Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions. |
| title_short | Basic regulatory principles of Escherichia coli's electron transport chain for varying oxygen conditions. |
| title_sort | basic regulatory principles of escherichia coli s electron transport chain for varying oxygen conditions |
| url | http://europepmc.org/articles/PMC4182436?pdf=render |
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