Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black Carbon
Nutrient enrichment caused by black carbon (BC) is a major ecological crisis in glacial ecosystems. The microbiological effects of BC were assessed in this study by using fluorescent fingerprinting assay based on flow cytometry (FCM) of bacterial communities with low (LNA) and high (HNA) nucleic aci...
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Frontiers Media S.A.
2022-02-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.844432/full |
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| author | Guannan Mao Mukan Ji Baiqing Xu Baiqing Xu Baiqing Xu Yongqin Liu Yongqin Liu Yongqin Liu Yongqin Liu Nianzhi Jiao |
| author_facet | Guannan Mao Mukan Ji Baiqing Xu Baiqing Xu Baiqing Xu Yongqin Liu Yongqin Liu Yongqin Liu Yongqin Liu Nianzhi Jiao |
| author_sort | Guannan Mao |
| collection | DOAJ |
| description | Nutrient enrichment caused by black carbon (BC) is a major ecological crisis in glacial ecosystems. The microbiological effects of BC were assessed in this study by using fluorescent fingerprinting assay based on flow cytometry (FCM) of bacterial communities with low (LNA) and high (HNA) nucleic acid-content bacteria. Here, we investigated a high-resolution temporal variation of bacterial abundance and LNA/HNA ratio in Tibetan ice cores. Our results revealed that bacterial abundance was proportional to the atmospheric BC on the glaciers. The shift of LNA functional groups to HNA functional groups in glaciers suggested BC emissions increased the proportion of highly active cells. In addition, distinct number of LNA and HNA functional groups was identified between the monsoon and non-monsoon seasons. Westerly winds with high amounts of BC accounted for high ratio of HNA functional groups during the non-monsoon season. In comparison, high moisture during the monsoon season decreased atmospheric BC loading, which increases the ratio of LNA functional groups. Correlations between BC and functional groups were very strong, showing that two functional groups may serve as early-warning indicators of microbiological effects of BC at low trophic level. Our approach provides a potential early-warning framework to study the influences of atmospheric BC on the glaciological community. |
| first_indexed | 2024-12-13T13:02:26Z |
| format | Article |
| id | doaj.art-ef89bb3f5f58492ab5fdbff7a814bbdf |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-13T13:02:26Z |
| publishDate | 2022-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-ef89bb3f5f58492ab5fdbff7a814bbdf2022-12-21T23:44:57ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-02-011310.3389/fmicb.2022.844432844432Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black CarbonGuannan Mao0Mukan Ji1Baiqing Xu2Baiqing Xu3Baiqing Xu4Yongqin Liu5Yongqin Liu6Yongqin Liu7Yongqin Liu8Nianzhi Jiao9Key Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, ChinaCenter for the Pan-Third Pole Environment, Lanzhou University, Lanzhou, ChinaKey Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, ChinaCAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaKey Laboratory of Tibetan Environment Changes and Land Surface Processes, Institute of Tibetan Plateau Research, Chinese Academy of Sciences, Beijing, ChinaCenter for the Pan-Third Pole Environment, Lanzhou University, Lanzhou, ChinaCAS Center for Excellence in Tibetan Plateau Earth Sciences, Chinese Academy of Sciences, Beijing, ChinaCollege of Resources and Environment, University of Chinese Academy of Sciences, Beijing, ChinaState Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, ChinaNutrient enrichment caused by black carbon (BC) is a major ecological crisis in glacial ecosystems. The microbiological effects of BC were assessed in this study by using fluorescent fingerprinting assay based on flow cytometry (FCM) of bacterial communities with low (LNA) and high (HNA) nucleic acid-content bacteria. Here, we investigated a high-resolution temporal variation of bacterial abundance and LNA/HNA ratio in Tibetan ice cores. Our results revealed that bacterial abundance was proportional to the atmospheric BC on the glaciers. The shift of LNA functional groups to HNA functional groups in glaciers suggested BC emissions increased the proportion of highly active cells. In addition, distinct number of LNA and HNA functional groups was identified between the monsoon and non-monsoon seasons. Westerly winds with high amounts of BC accounted for high ratio of HNA functional groups during the non-monsoon season. In comparison, high moisture during the monsoon season decreased atmospheric BC loading, which increases the ratio of LNA functional groups. Correlations between BC and functional groups were very strong, showing that two functional groups may serve as early-warning indicators of microbiological effects of BC at low trophic level. Our approach provides a potential early-warning framework to study the influences of atmospheric BC on the glaciological community.https://www.frontiersin.org/articles/10.3389/fmicb.2022.844432/fullblack carbonbacteria functional groupsearly-warning indicatorsTibetan Plateautemporal variationice cores |
| spellingShingle | Guannan Mao Mukan Ji Baiqing Xu Baiqing Xu Baiqing Xu Yongqin Liu Yongqin Liu Yongqin Liu Yongqin Liu Nianzhi Jiao Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black Carbon Frontiers in Microbiology black carbon bacteria functional groups early-warning indicators Tibetan Plateau temporal variation ice cores |
| title | Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black Carbon |
| title_full | Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black Carbon |
| title_fullStr | Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black Carbon |
| title_full_unstemmed | Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black Carbon |
| title_short | Variation of High and Low Nucleic Acid-Content Bacteria in Tibetan Ice Cores and Their Relationship to Black Carbon |
| title_sort | variation of high and low nucleic acid content bacteria in tibetan ice cores and their relationship to black carbon |
| topic | black carbon bacteria functional groups early-warning indicators Tibetan Plateau temporal variation ice cores |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.844432/full |
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