Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations
Optokinetic reflex (OKR) assays in zebrafish models are a valuable tool for studying a diverse range of ophthalmological and neurological conditions. Despite its increasing popularity in recent years, there are no clear reporting guidelines for the assay. Following reporting guidelines in research e...
| Main Authors: | , , , , , |
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| Format: | Article |
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MDPI AG
2023-12-01
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| Series: | Biology |
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| Online Access: | https://www.mdpi.com/2079-7737/13/1/4 |
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| author | Vanessa Rodwell Manjiri Patil Helen J. Kuht Stephan C. F. Neuhauss William H. J. Norton Mervyn G. Thomas |
| author_facet | Vanessa Rodwell Manjiri Patil Helen J. Kuht Stephan C. F. Neuhauss William H. J. Norton Mervyn G. Thomas |
| author_sort | Vanessa Rodwell |
| collection | DOAJ |
| description | Optokinetic reflex (OKR) assays in zebrafish models are a valuable tool for studying a diverse range of ophthalmological and neurological conditions. Despite its increasing popularity in recent years, there are no clear reporting guidelines for the assay. Following reporting guidelines in research enhances reproducibility, reduces bias, and mitigates underreporting and poor methodologies in published works. To better understand optimal reporting standards for an OKR assay in zebrafish, we performed a systematic literature review exploring the animal, environmental, and technical factors that should be considered. Using search criteria from three online databases, a total of 109 research papers were selected for review. Multiple crucial factors were identified, including larval characteristics, sample size, fixing method, OKR set-up, distance of stimulus, detailed stimulus parameters, eye recording, and eye movement analysis. The outcome of the literature analysis highlighted the insufficient information provided in past research papers and the lack of a systematic way to present the parameters related to each of the experimental factors. To circumvent any future errors and champion robust transparent research, we have created the zebrafish optokinetic (ZOK) reflex minimal reporting guideline. |
| first_indexed | 2024-03-08T11:05:50Z |
| format | Article |
| id | doaj.art-ef8fc2e77e9e4cfcb424c600e75cbe39 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-08T11:05:50Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-ef8fc2e77e9e4cfcb424c600e75cbe392024-01-26T15:06:32ZengMDPI AGBiology2079-77372023-12-01131410.3390/biology13010004Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and RecommendationsVanessa Rodwell0Manjiri Patil1Helen J. Kuht2Stephan C. F. Neuhauss3William H. J. Norton4Mervyn G. Thomas5Ulverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, Leicester LE1 7RH, UKUlverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, Leicester LE1 7RH, UKUlverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, Leicester LE1 7RH, UKDepartment of Molecular Life Science, University of Zurich, 8006 Zurich, SwitzerlandDepartment of Genetics and Genome Biology, University of Leicester, Leicester LE1 7RH, UKUlverscroft Eye Unit, School of Psychology and Vision Sciences, University of Leicester, Leicester LE1 7RH, UKOptokinetic reflex (OKR) assays in zebrafish models are a valuable tool for studying a diverse range of ophthalmological and neurological conditions. Despite its increasing popularity in recent years, there are no clear reporting guidelines for the assay. Following reporting guidelines in research enhances reproducibility, reduces bias, and mitigates underreporting and poor methodologies in published works. To better understand optimal reporting standards for an OKR assay in zebrafish, we performed a systematic literature review exploring the animal, environmental, and technical factors that should be considered. Using search criteria from three online databases, a total of 109 research papers were selected for review. Multiple crucial factors were identified, including larval characteristics, sample size, fixing method, OKR set-up, distance of stimulus, detailed stimulus parameters, eye recording, and eye movement analysis. The outcome of the literature analysis highlighted the insufficient information provided in past research papers and the lack of a systematic way to present the parameters related to each of the experimental factors. To circumvent any future errors and champion robust transparent research, we have created the zebrafish optokinetic (ZOK) reflex minimal reporting guideline.https://www.mdpi.com/2079-7737/13/1/4OKROKNoptokineticbehavioural assayzebrafishdanio rerio |
| spellingShingle | Vanessa Rodwell Manjiri Patil Helen J. Kuht Stephan C. F. Neuhauss William H. J. Norton Mervyn G. Thomas Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations Biology OKR OKN optokinetic behavioural assay zebrafish danio rerio |
| title | Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations |
| title_full | Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations |
| title_fullStr | Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations |
| title_full_unstemmed | Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations |
| title_short | Zebrafish Optokinetic Reflex: Minimal Reporting Guidelines and Recommendations |
| title_sort | zebrafish optokinetic reflex minimal reporting guidelines and recommendations |
| topic | OKR OKN optokinetic behavioural assay zebrafish danio rerio |
| url | https://www.mdpi.com/2079-7737/13/1/4 |
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