Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India.
Mosquitoes are known to harbor a large number of insect specific viruses (ISV) in addition to viruses of public health importance. These ISVs are highly species specific and are non-pathogenic to humans or domestic animals. However, there is a potential threat of these ISVs evolving into human patho...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0277276 |
| _version_ | 1797956073881075712 |
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| author | Kavita Lole Ashwini Ramdasi Sucheta Patil Shivani Thakar Amol Nath Onkar Ghuge Abhranil Gangopadhayya Anakkathil B Sudeep Sarah Cherian |
| author_facet | Kavita Lole Ashwini Ramdasi Sucheta Patil Shivani Thakar Amol Nath Onkar Ghuge Abhranil Gangopadhayya Anakkathil B Sudeep Sarah Cherian |
| author_sort | Kavita Lole |
| collection | DOAJ |
| description | Mosquitoes are known to harbor a large number of insect specific viruses (ISV) in addition to viruses of public health importance. These ISVs are highly species specific and are non-pathogenic to humans or domestic animals. However, there is a potential threat of these ISVs evolving into human pathogens by genome alterations. Some ISVs are known to modulate replication of pathogenic viruses by altering the susceptibility of vector mosquitoes to pathogenic viruses, thereby either inhibiting or enhancing transmission of the latter. In the present study, we report predominance of Phasi Charoen-like virus (PCLV, Family: Phenuviridae) contributing to >60% of the total reads in Aedes aegypti mosquitoes collected from Pune district of Maharashtra state using next generation sequencing based metagenomic analysis of viromes. Similar results were also obtained with mosquitoes from Assam, Tamil Nadu and Karnataka states of India. Comparison of Pune mosquito sequences with PCLV Rio (Brazil) isolate showed 98.90%, 99.027% and 98.88% homologies in the S, M and L segments respectively indicating less genetic heterogeneity of PCLV. The study also demonstrated occurrence of transovarial transmission as seen by detection of PCLV in eggs, larvae, pupae and male mosquitoes. Ae. aegypti mosquitoes collected from Pune also showed a large number of reads for viruses belonging to Baculoviridae, Rhabdoviridae, Genomoviridae and Bunyaviridae families. The role of PCLV in the replication of dengue and chikungunya virus is yet not clear. It warrants further studies to know the significance of PCLV and other ISVs on the replication and transmission of Ae. aegypti borne pathogenic viruses, especially in the absence of prophylactics or therapeutics. |
| first_indexed | 2024-04-10T23:44:14Z |
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| id | doaj.art-ef93b2159b6d4cc9b66b315a6a1aa2d4 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-04-10T23:44:14Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-ef93b2159b6d4cc9b66b315a6a1aa2d42023-01-11T05:32:33ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-011712e027727610.1371/journal.pone.0277276Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India.Kavita LoleAshwini RamdasiSucheta PatilShivani ThakarAmol NathOnkar GhugeAbhranil GangopadhayyaAnakkathil B SudeepSarah CherianMosquitoes are known to harbor a large number of insect specific viruses (ISV) in addition to viruses of public health importance. These ISVs are highly species specific and are non-pathogenic to humans or domestic animals. However, there is a potential threat of these ISVs evolving into human pathogens by genome alterations. Some ISVs are known to modulate replication of pathogenic viruses by altering the susceptibility of vector mosquitoes to pathogenic viruses, thereby either inhibiting or enhancing transmission of the latter. In the present study, we report predominance of Phasi Charoen-like virus (PCLV, Family: Phenuviridae) contributing to >60% of the total reads in Aedes aegypti mosquitoes collected from Pune district of Maharashtra state using next generation sequencing based metagenomic analysis of viromes. Similar results were also obtained with mosquitoes from Assam, Tamil Nadu and Karnataka states of India. Comparison of Pune mosquito sequences with PCLV Rio (Brazil) isolate showed 98.90%, 99.027% and 98.88% homologies in the S, M and L segments respectively indicating less genetic heterogeneity of PCLV. The study also demonstrated occurrence of transovarial transmission as seen by detection of PCLV in eggs, larvae, pupae and male mosquitoes. Ae. aegypti mosquitoes collected from Pune also showed a large number of reads for viruses belonging to Baculoviridae, Rhabdoviridae, Genomoviridae and Bunyaviridae families. The role of PCLV in the replication of dengue and chikungunya virus is yet not clear. It warrants further studies to know the significance of PCLV and other ISVs on the replication and transmission of Ae. aegypti borne pathogenic viruses, especially in the absence of prophylactics or therapeutics.https://doi.org/10.1371/journal.pone.0277276 |
| spellingShingle | Kavita Lole Ashwini Ramdasi Sucheta Patil Shivani Thakar Amol Nath Onkar Ghuge Abhranil Gangopadhayya Anakkathil B Sudeep Sarah Cherian Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India. PLoS ONE |
| title | Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India. |
| title_full | Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India. |
| title_fullStr | Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India. |
| title_full_unstemmed | Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India. |
| title_short | Abundance of Phasi-Charoen-like virus in Aedes aegypti mosquito populations in different states of India. |
| title_sort | abundance of phasi charoen like virus in aedes aegypti mosquito populations in different states of india |
| url | https://doi.org/10.1371/journal.pone.0277276 |
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