Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer
Gynura procumbens is widely used as traditional remedy in South-East Asia. Gynura procumbens exhibites anti inflammatory, antioxidant, and reduced blood pressure activity. The aim of this study was to determine chromatographic profile of ethyl acetate fraction of Gynura procumbens (FEG) and to inves...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Indonesian Society for Cancer Chemoprevention
2011-10-01
|
| Series: | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| Online Access: | https://ijcc.chemoprev.org/index.php/ijcc/article/view/42 |
| _version_ | 1811231766152740864 |
|---|---|
| author | Nunuk Aries Nurulita Edy Meiyanto Sugiyanto Sugiyanto |
| author_facet | Nunuk Aries Nurulita Edy Meiyanto Sugiyanto Sugiyanto |
| author_sort | Nunuk Aries Nurulita |
| collection | DOAJ |
| description | Gynura procumbens is widely used as traditional remedy in South-East Asia. Gynura procumbens exhibites anti inflammatory, antioxidant, and reduced blood pressure activity. The aim of this study was to determine chromatographic profile of ethyl acetate fraction of Gynura procumbens (FEG) and to investigate its cytotoxic properties and selectivity to colon cancerand breast cancer cancer cells. The chromatographic profile of FEG was determined using HPTLC densitometric and HPLC. MTT (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed to determine the growth inhibitory effect of FEG on the growth of WiDr, MCF-7, and T47D cells. NIH3T3, a normal cells was used to determine the selectivity of FEG, which contained small amount of quercetin as identified from chromatographic profile both HPTLC and HPLC. FEG inhibited cell growth of WiDr, of MCF-7 and of T47D cells in time dependent manner. Quercetin affected cell growth inhibition approximately two fold higher at WiDr and MCF-7, whereas FEG had lower effect on T47D cell. Quercetin did not seem as the main active compound of FEG. At this study, FEG caused less inhibition on the growth of NIH3T3 cells than that of on all cell lines. Selectivity index (SI) of FEG on WiDr, MCF-7 and T47D were 4.97, 2.77 and 7.79 respectively. According to the datas obtained, FEG possesses moderate to high cytotoxicity properties on WiDr, MCF-7 and T47D cells. FEG demonstrates selective effect against cancer cells and reveals prospective properties as cancer chemoprevention agent.
Keywords: Gynura procumbens, colon cancer, breast cancer, cytotoxicity, selectivity |
| first_indexed | 2024-04-12T10:50:33Z |
| format | Article |
| id | doaj.art-ef93ffc3247d4a30b3c353bf1d712255 |
| institution | Directory Open Access Journal |
| issn | 2088-0197 2355-8989 |
| language | English |
| last_indexed | 2024-04-12T10:50:33Z |
| publishDate | 2011-10-01 |
| publisher | Indonesian Society for Cancer Chemoprevention |
| record_format | Article |
| series | ISCC (Indonesian Journal of Cancer Chemoprevention) |
| spelling | doaj.art-ef93ffc3247d4a30b3c353bf1d7122552022-12-22T03:36:16ZengIndonesian Society for Cancer ChemopreventionISCC (Indonesian Journal of Cancer Chemoprevention)2088-01972355-89892011-10-012327428010.14499/indonesianjcanchemoprev2iss3pp274-28043Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast CancerNunuk Aries Nurulita0Edy Meiyanto1Sugiyanto Sugiyanto2Faculty of Pharmacy, Universitas Muhammadiyah PurwokertoCancer Chemoprevention Research Center, Faculty of Pharmacy, Universitas Gadjah MadaDepartment of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah MadaGynura procumbens is widely used as traditional remedy in South-East Asia. Gynura procumbens exhibites anti inflammatory, antioxidant, and reduced blood pressure activity. The aim of this study was to determine chromatographic profile of ethyl acetate fraction of Gynura procumbens (FEG) and to investigate its cytotoxic properties and selectivity to colon cancerand breast cancer cancer cells. The chromatographic profile of FEG was determined using HPTLC densitometric and HPLC. MTT (3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay was performed to determine the growth inhibitory effect of FEG on the growth of WiDr, MCF-7, and T47D cells. NIH3T3, a normal cells was used to determine the selectivity of FEG, which contained small amount of quercetin as identified from chromatographic profile both HPTLC and HPLC. FEG inhibited cell growth of WiDr, of MCF-7 and of T47D cells in time dependent manner. Quercetin affected cell growth inhibition approximately two fold higher at WiDr and MCF-7, whereas FEG had lower effect on T47D cell. Quercetin did not seem as the main active compound of FEG. At this study, FEG caused less inhibition on the growth of NIH3T3 cells than that of on all cell lines. Selectivity index (SI) of FEG on WiDr, MCF-7 and T47D were 4.97, 2.77 and 7.79 respectively. According to the datas obtained, FEG possesses moderate to high cytotoxicity properties on WiDr, MCF-7 and T47D cells. FEG demonstrates selective effect against cancer cells and reveals prospective properties as cancer chemoprevention agent. Keywords: Gynura procumbens, colon cancer, breast cancer, cytotoxicity, selectivityhttps://ijcc.chemoprev.org/index.php/ijcc/article/view/42 |
| spellingShingle | Nunuk Aries Nurulita Edy Meiyanto Sugiyanto Sugiyanto Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer ISCC (Indonesian Journal of Cancer Chemoprevention) |
| title | Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer |
| title_full | Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer |
| title_fullStr | Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer |
| title_full_unstemmed | Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer |
| title_short | Selectivity of Ethyl Acetate Fraction of Gynura Procumbens on Colon Cancer and Breast Cancer |
| title_sort | selectivity of ethyl acetate fraction of gynura procumbens on colon cancer and breast cancer |
| url | https://ijcc.chemoprev.org/index.php/ijcc/article/view/42 |
| work_keys_str_mv | AT nunukariesnurulita selectivityofethylacetatefractionofgynuraprocumbensoncoloncancerandbreastcancer AT edymeiyanto selectivityofethylacetatefractionofgynuraprocumbensoncoloncancerandbreastcancer AT sugiyantosugiyanto selectivityofethylacetatefractionofgynuraprocumbensoncoloncancerandbreastcancer |