Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets
Breast cancer ranked second among most frequent cancer in the world playing a significant role in mortality rate. Having prior knowledge on differentially expressed genes in breast cell carcinoma elucidated important indications to understand the molecular mechanism underneath breast carcinogenesis....
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| Format: | Article |
| Language: | English |
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Elsevier
2020-12-01
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| Series: | Biochemistry and Biophysics Reports |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405580820301771 |
| _version_ | 1818644033264680960 |
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| author | Mohsina Akter Mou Nawshin Atia Keya Majharul Islam Md. Jahid Hossain Md. Syeed Al Habib Rahat Alam Sohel Rana Abdus Samad Foysal Ahammad |
| author_facet | Mohsina Akter Mou Nawshin Atia Keya Majharul Islam Md. Jahid Hossain Md. Syeed Al Habib Rahat Alam Sohel Rana Abdus Samad Foysal Ahammad |
| author_sort | Mohsina Akter Mou |
| collection | DOAJ |
| description | Breast cancer ranked second among most frequent cancer in the world playing a significant role in mortality rate. Having prior knowledge on differentially expressed genes in breast cell carcinoma elucidated important indications to understand the molecular mechanism underneath breast carcinogenesis. In this study we have investigated the distinguished CSN1S1 expression in human breast cancer. We have analyzed CSN1S1 mRNA expression between cancer and normal tissues using TCGA datasets. Moreover, analysis including promoter methylation, mutations, prognosis, co-expression, gene ontology, and pathways of CSN1S1 were performed by the TCGA Wanderer, UCSC Xena, cBioPortal, PrognoScan, UALCAN, and Enricher server. We have observed low mRNA expression and high promoter methylation of CSN1S1 in cancer tissues compared to normal tissues. Furthermore, we have also identified low mRNA expression in clinicopathological patients, as well as 9 deleterious mutations with highly co-expressed protein MRC1, and significantly related signaling pathways. We have found a positive correlation between the lower expression of CSN1S1 and patients surviving with breast cancer. Here we have concluded that CSN1S1 acts as a biomarker for the surveillance and prognosis of breast cancer, and also works as a novel therapeutic target at the molecular and pathway levels. |
| first_indexed | 2024-12-17T00:08:25Z |
| format | Article |
| id | doaj.art-ef9456899d0c43b4afe8c7add374dce5 |
| institution | Directory Open Access Journal |
| issn | 2405-5808 |
| language | English |
| last_indexed | 2024-12-17T00:08:25Z |
| publishDate | 2020-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biochemistry and Biophysics Reports |
| spelling | doaj.art-ef9456899d0c43b4afe8c7add374dce52022-12-21T22:10:53ZengElsevierBiochemistry and Biophysics Reports2405-58082020-12-0124100867Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasetsMohsina Akter Mou0Nawshin Atia Keya1Majharul Islam2Md. Jahid Hossain3Md. Syeed Al Habib4Rahat Alam5Sohel Rana6Abdus Samad7Foysal Ahammad8Department of Genetic Engineering and Biotechnology, Shahjalal University of Science and Technology, BangladeshDepartment of Microbiology, Noakhali Science and Technology University, BangladeshDepartment of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail, 1902, BangladeshMaternal and Child Health Division (MCHD), Icddrb, BangladeshDepartment of Biochemistry and Molecular Biology, Mawlana Bhashani Science and Technology University, Tangail, 1902, BangladeshDepartment of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh; Laboraty of Computational Biology, Biological Solution Cantre (BiolSol Centre), Dhaka, BangladeshDepartment of Pharmacy, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, BangladeshDepartment of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh; Laboraty of Computational Biology, Biological Solution Cantre (BiolSol Centre), Dhaka, Bangladesh; Corresponding author. Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh.Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh; Laboraty of Computational Biology, Biological Solution Cantre (BiolSol Centre), Dhaka, Bangladesh; Department of Biological Sciences, Faculty of Science, King Abdulaziz University (KAU), Jeddah, 21589, Saudi Arabia; Corresponding author. Department of Genetic Engineering and Biotechnology, Faculty of Biological Sciences and Technology, Jashore University of Science and Technology, Jashore, 7408, Bangladesh.Breast cancer ranked second among most frequent cancer in the world playing a significant role in mortality rate. Having prior knowledge on differentially expressed genes in breast cell carcinoma elucidated important indications to understand the molecular mechanism underneath breast carcinogenesis. In this study we have investigated the distinguished CSN1S1 expression in human breast cancer. We have analyzed CSN1S1 mRNA expression between cancer and normal tissues using TCGA datasets. Moreover, analysis including promoter methylation, mutations, prognosis, co-expression, gene ontology, and pathways of CSN1S1 were performed by the TCGA Wanderer, UCSC Xena, cBioPortal, PrognoScan, UALCAN, and Enricher server. We have observed low mRNA expression and high promoter methylation of CSN1S1 in cancer tissues compared to normal tissues. Furthermore, we have also identified low mRNA expression in clinicopathological patients, as well as 9 deleterious mutations with highly co-expressed protein MRC1, and significantly related signaling pathways. We have found a positive correlation between the lower expression of CSN1S1 and patients surviving with breast cancer. Here we have concluded that CSN1S1 acts as a biomarker for the surveillance and prognosis of breast cancer, and also works as a novel therapeutic target at the molecular and pathway levels.http://www.sciencedirect.com/science/article/pii/S2405580820301771CSN1S1Breast cancerMutational analysisBiomarkerTranscriptional expression |
| spellingShingle | Mohsina Akter Mou Nawshin Atia Keya Majharul Islam Md. Jahid Hossain Md. Syeed Al Habib Rahat Alam Sohel Rana Abdus Samad Foysal Ahammad Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets Biochemistry and Biophysics Reports CSN1S1 Breast cancer Mutational analysis Biomarker Transcriptional expression |
| title | Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets |
| title_full | Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets |
| title_fullStr | Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets |
| title_full_unstemmed | Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets |
| title_short | Validation of CSN1S1 transcriptional expression, promoter methylation, and prognostic power in breast cancer using independent datasets |
| title_sort | validation of csn1s1 transcriptional expression promoter methylation and prognostic power in breast cancer using independent datasets |
| topic | CSN1S1 Breast cancer Mutational analysis Biomarker Transcriptional expression |
| url | http://www.sciencedirect.com/science/article/pii/S2405580820301771 |
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