The impact of short-term acute heat stress on the rumen microbiome of Hanwoo steers
Heat stress negatively affects cattle productivity by reducing feed intake. In the present study, we assessed if the rumen microbiome composition of Hanwoo steers was altered by exposure to heat stress. Rumen samples were collected from fou...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Korean Society of Animal Sciences and Technology
2020-03-01
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| Series: | Journal of Animal Science and Technology |
| Subjects: | |
| Online Access: | http://www.ejast.org/archive/view_article?pid=jast-62-2-208 |
| _version_ | 1828403354550665216 |
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| author | Youl Chang Baek Hyuck Choi Jin-Young Jeong Sung Dae Lee Min Ji Kim Seul Lee Sang-Yun Ji Minseok Kim |
| author_facet | Youl Chang Baek Hyuck Choi Jin-Young Jeong Sung Dae Lee Min Ji Kim Seul Lee Sang-Yun Ji Minseok Kim |
| author_sort | Youl Chang Baek |
| collection | DOAJ |
| description | Heat stress negatively affects cattle productivity by reducing feed intake. In
the present study, we assessed if the rumen microbiome composition of Hanwoo
steers was altered by exposure to heat stress. Rumen samples were collected from
four Hanwoo steers that were individually housed in climate-controlled chambers
with 60% humidity and environmental temperatures of: 1) 15°C (0-day
group), 2) 35°C for 3 days (3-day group), and 3) 35°C for 6 days
(6-day group). The total community DNA of samples was extracted, and 997,843
bacterial and 1,508,770 archaeal sequences were analyzed using next-generation
sequencing. Assessment of the relative abundances revealed 15 major phyla of
which Bacteroidetes was found to be the most dominant. After 3 days of heat
stress exposure there were no significant changes in the rumen microbiome
composition, except for a decrease in the Planctomycetes. However, after 6 days
of heat stress exposure, we found that the relative abundance of fibrolytic
Ruminococcaceae had decreased while that of lactate-producing Lactobacillaceae
and amylolytic Prevotella and Ruminobacter had
increased. The normal rumen microbiome of Hanwoo cattle was shown to be
disrupted after 6 days of heat stress, which led to the decrease in fibrolytic
bacteria that are sensitive to low pH and the increase in both lactate-producing
and amylolytic bacteria. We have demonstrated that the microbiome composition of
the rumen is affected by acute heat stress. Our findings may contribute to the
development of different feeding strategies to restore heat stress-induced
disruption of the rumen microbiome. |
| first_indexed | 2024-12-10T10:16:32Z |
| format | Article |
| id | doaj.art-ef979f892a46414e8a9bc8ccf436ce0d |
| institution | Directory Open Access Journal |
| issn | 2672-0191 2055-0391 |
| language | English |
| last_indexed | 2024-12-10T10:16:32Z |
| publishDate | 2020-03-01 |
| publisher | Korean Society of Animal Sciences and Technology |
| record_format | Article |
| series | Journal of Animal Science and Technology |
| spelling | doaj.art-ef979f892a46414e8a9bc8ccf436ce0d2022-12-22T01:52:59ZengKorean Society of Animal Sciences and TechnologyJournal of Animal Science and Technology2672-01912055-03912020-03-0162220821710.5187/jast.2020.62.2.208jast-62-2-208The impact of short-term acute heat stress on the rumen microbiome of Hanwoo steersYoul Chang Baek0Hyuck Choi1Jin-Young Jeong2Sung Dae Lee3Min Ji Kim4Seul Lee5Sang-Yun Ji6Minseok Kim7Animal Nutrition & Physiology Team, National Institute of Animal Science, Department of Pet Science, Seojeong University, Animal Nutrition & Physiology Team, National Institute of Animal Science, Animal Nutrition & Physiology Team, National Institute of Animal Science, Animal Nutrition & Physiology Team, National Institute of Animal Science, Animal Nutrition & Physiology Team, National Institute of Animal Science, Animal Nutrition & Physiology Team, National Institute of Animal Science, Department of Animal Science, College of Agriculture and Life Sciences, Chonnam National University, Heat stress negatively affects cattle productivity by reducing feed intake. In the present study, we assessed if the rumen microbiome composition of Hanwoo steers was altered by exposure to heat stress. Rumen samples were collected from four Hanwoo steers that were individually housed in climate-controlled chambers with 60% humidity and environmental temperatures of: 1) 15°C (0-day group), 2) 35°C for 3 days (3-day group), and 3) 35°C for 6 days (6-day group). The total community DNA of samples was extracted, and 997,843 bacterial and 1,508,770 archaeal sequences were analyzed using next-generation sequencing. Assessment of the relative abundances revealed 15 major phyla of which Bacteroidetes was found to be the most dominant. After 3 days of heat stress exposure there were no significant changes in the rumen microbiome composition, except for a decrease in the Planctomycetes. However, after 6 days of heat stress exposure, we found that the relative abundance of fibrolytic Ruminococcaceae had decreased while that of lactate-producing Lactobacillaceae and amylolytic Prevotella and Ruminobacter had increased. The normal rumen microbiome of Hanwoo cattle was shown to be disrupted after 6 days of heat stress, which led to the decrease in fibrolytic bacteria that are sensitive to low pH and the increase in both lactate-producing and amylolytic bacteria. We have demonstrated that the microbiome composition of the rumen is affected by acute heat stress. Our findings may contribute to the development of different feeding strategies to restore heat stress-induced disruption of the rumen microbiome.http://www.ejast.org/archive/view_article?pid=jast-62-2-208climate-controlled chamberenvironmental temperaturehumiditynext-generation sequencingrumen microbiome composition |
| spellingShingle | Youl Chang Baek Hyuck Choi Jin-Young Jeong Sung Dae Lee Min Ji Kim Seul Lee Sang-Yun Ji Minseok Kim The impact of short-term acute heat stress on the rumen microbiome of Hanwoo steers Journal of Animal Science and Technology climate-controlled chamber environmental temperature humidity next-generation sequencing rumen microbiome composition |
| title | The impact of short-term acute heat stress on the rumen microbiome of
Hanwoo steers |
| title_full | The impact of short-term acute heat stress on the rumen microbiome of
Hanwoo steers |
| title_fullStr | The impact of short-term acute heat stress on the rumen microbiome of
Hanwoo steers |
| title_full_unstemmed | The impact of short-term acute heat stress on the rumen microbiome of
Hanwoo steers |
| title_short | The impact of short-term acute heat stress on the rumen microbiome of
Hanwoo steers |
| title_sort | impact of short term acute heat stress on the rumen microbiome of hanwoo steers |
| topic | climate-controlled chamber environmental temperature humidity next-generation sequencing rumen microbiome composition |
| url | http://www.ejast.org/archive/view_article?pid=jast-62-2-208 |
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