The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl.
The leaf-explants of Saintpaulia ionantha Wendl. var.'miniature' were treated by different doses of MNH and cultured on shoot regeneration medium. A strong toxic effect of some MNH doses on explant survival during the first two subcultures was noticed. The explants surviving treatment rege...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Polish Botanical Society
2014-01-01
|
| Series: | Acta Societatis Botanicorum Poloniae |
| Subjects: | |
| Online Access: | https://pbsociety.org.pl/journals/index.php/asbp/article/view/953 |
| _version_ | 1818995404130222080 |
|---|---|
| author | Marek Gaj Małgorzata D. Gaj |
| author_facet | Marek Gaj Małgorzata D. Gaj |
| author_sort | Marek Gaj |
| collection | DOAJ |
| description | The leaf-explants of Saintpaulia ionantha Wendl. var.'miniature' were treated by different doses of MNH and cultured on shoot regeneration medium. A strong toxic effect of some MNH doses on explant survival during the first two subcultures was noticed. The explants surviving treatment regenerated shoots with the efficiency comparable to the control. The high number of shoots regenerated from mutagenised leaves showed chlorophyll chimerism (so-called variegated forms). The use of 5 mM MNH for 1.5 or 2 h was found very effective, as 100% of survived explants regenerated variegated shoots. Besides hundreds of variegated forms also leaf-shape and flower-colour variants were observed in MNH-treated culture. Somaclonal variation was not observed in the control culture. The results indicate the great efficiency of in vitro applied MNH for in-duction of morphological variants of Saintpaulia, and especially variegated forms. |
| first_indexed | 2024-12-20T21:13:18Z |
| format | Article |
| id | doaj.art-ef98ad9ed99a4041b1c12a00689a913a |
| institution | Directory Open Access Journal |
| issn | 2083-9480 |
| language | English |
| last_indexed | 2024-12-20T21:13:18Z |
| publishDate | 2014-01-01 |
| publisher | Polish Botanical Society |
| record_format | Article |
| series | Acta Societatis Botanicorum Poloniae |
| spelling | doaj.art-ef98ad9ed99a4041b1c12a00689a913a2022-12-21T19:26:29ZengPolish Botanical SocietyActa Societatis Botanicorum Poloniae2083-94802014-01-01653-433934310.5586/asbp.1996.052726The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl.Marek Gaj0Małgorzata D. Gaj1University of SilesiaUniversity of SilesiaThe leaf-explants of Saintpaulia ionantha Wendl. var.'miniature' were treated by different doses of MNH and cultured on shoot regeneration medium. A strong toxic effect of some MNH doses on explant survival during the first two subcultures was noticed. The explants surviving treatment regenerated shoots with the efficiency comparable to the control. The high number of shoots regenerated from mutagenised leaves showed chlorophyll chimerism (so-called variegated forms). The use of 5 mM MNH for 1.5 or 2 h was found very effective, as 100% of survived explants regenerated variegated shoots. Besides hundreds of variegated forms also leaf-shape and flower-colour variants were observed in MNH-treated culture. Somaclonal variation was not observed in the control culture. The results indicate the great efficiency of in vitro applied MNH for in-duction of morphological variants of Saintpaulia, and especially variegated forms.https://pbsociety.org.pl/journals/index.php/asbp/article/view/953African violetMNHleaf culturechlorophyll deficient formsorganogenesis |
| spellingShingle | Marek Gaj Małgorzata D. Gaj The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl. Acta Societatis Botanicorum Poloniae African violet MNH leaf culture chlorophyll deficient forms organogenesis |
| title | The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl. |
| title_full | The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl. |
| title_fullStr | The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl. |
| title_full_unstemmed | The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl. |
| title_short | The high frequency of variegated forms after in vitro mutagenesis in Saintpaulia ionantha Wendl. |
| title_sort | high frequency of variegated forms after in vitro mutagenesis in saintpaulia ionantha wendl |
| topic | African violet MNH leaf culture chlorophyll deficient forms organogenesis |
| url | https://pbsociety.org.pl/journals/index.php/asbp/article/view/953 |
| work_keys_str_mv | AT marekgaj thehighfrequencyofvariegatedformsafterinvitromutagenesisinsaintpauliaionanthawendl AT małgorzatadgaj thehighfrequencyofvariegatedformsafterinvitromutagenesisinsaintpauliaionanthawendl AT marekgaj highfrequencyofvariegatedformsafterinvitromutagenesisinsaintpauliaionanthawendl AT małgorzatadgaj highfrequencyofvariegatedformsafterinvitromutagenesisinsaintpauliaionanthawendl |