Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes
Glucose is a major energy substrate for porcine adipocytes and also serves as a regulatory signal for adipogenesis and lipid metabolism. In this study, we combined transcriptome and metabolome analyses to reveal the underlying regulatory mechanisms of high glucose (HG) on adipogenesis by comparing d...
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MDPI AG
2024-03-01
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| Series: | Current Issues in Molecular Biology |
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| Online Access: | https://www.mdpi.com/1467-3045/46/3/131 |
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| author | Susu Jiang Guohua Zhang Jian Miao Dianhu Wu Ximei Li Jiawei Li Jianxiong Lu Shuangbao Gun |
| author_facet | Susu Jiang Guohua Zhang Jian Miao Dianhu Wu Ximei Li Jiawei Li Jianxiong Lu Shuangbao Gun |
| author_sort | Susu Jiang |
| collection | DOAJ |
| description | Glucose is a major energy substrate for porcine adipocytes and also serves as a regulatory signal for adipogenesis and lipid metabolism. In this study, we combined transcriptome and metabolome analyses to reveal the underlying regulatory mechanisms of high glucose (HG) on adipogenesis by comparing differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) identified in porcine adipocytes. Results showed that HG (20 mmol/L) significantly increased fat accumulation in porcine adipocytes compared to low glucose (LG, 5 mmol/L). A total of 843 DEGs and 365 DAMs were identified. Functional enrichment analyses of DEGs found that multiple pathways were related to adipogenesis, lipid metabolism, and immune-inflammatory responses. PPARγ, C/EBPα, ChREBP, and FOS were identified as the key hub genes through module 3 analysis, and PPARγ acted as a central regulator by linking genes involved in lipid metabolism and immune-inflammatory responses. Gene-metabolite networks found that PPARγ-13-HODE was the most important interaction relationship. These results revealed that PPARγ could mediate the cross-talk between adipogenesis and the immune-inflammatory response during adipocyte maturation. This work provides a comprehensive view of the regulatory mechanisms of glucose on adipogenesis in porcine adipocytes. |
| first_indexed | 2024-04-24T18:26:13Z |
| format | Article |
| id | doaj.art-ef9b49608f5a47359f4504308b2942ee |
| institution | Directory Open Access Journal |
| issn | 1467-3037 1467-3045 |
| language | English |
| last_indexed | 2024-04-24T18:26:13Z |
| publishDate | 2024-03-01 |
| publisher | MDPI AG |
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| spelling | doaj.art-ef9b49608f5a47359f4504308b2942ee2024-03-27T13:31:23ZengMDPI AGCurrent Issues in Molecular Biology1467-30371467-30452024-03-014632027204210.3390/cimb46030131Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine AdipocytesSusu Jiang0Guohua Zhang1Jian Miao2Dianhu Wu3Ximei Li4Jiawei Li5Jianxiong Lu6Shuangbao Gun7College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, ChinaCollege of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, ChinaCollege of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, ChinaCollege of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, ChinaCollege of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, ChinaCollege of Life Science and Engineering, Northwest Minzu University, Lanzhou 730030, ChinaCollege of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070, ChinaGlucose is a major energy substrate for porcine adipocytes and also serves as a regulatory signal for adipogenesis and lipid metabolism. In this study, we combined transcriptome and metabolome analyses to reveal the underlying regulatory mechanisms of high glucose (HG) on adipogenesis by comparing differentially expressed genes (DEGs) and differentially accumulated metabolites (DAMs) identified in porcine adipocytes. Results showed that HG (20 mmol/L) significantly increased fat accumulation in porcine adipocytes compared to low glucose (LG, 5 mmol/L). A total of 843 DEGs and 365 DAMs were identified. Functional enrichment analyses of DEGs found that multiple pathways were related to adipogenesis, lipid metabolism, and immune-inflammatory responses. PPARγ, C/EBPα, ChREBP, and FOS were identified as the key hub genes through module 3 analysis, and PPARγ acted as a central regulator by linking genes involved in lipid metabolism and immune-inflammatory responses. Gene-metabolite networks found that PPARγ-13-HODE was the most important interaction relationship. These results revealed that PPARγ could mediate the cross-talk between adipogenesis and the immune-inflammatory response during adipocyte maturation. This work provides a comprehensive view of the regulatory mechanisms of glucose on adipogenesis in porcine adipocytes.https://www.mdpi.com/1467-3045/46/3/131pigadipogenesisglucosetranscriptomemetabolomefat accumulation |
| spellingShingle | Susu Jiang Guohua Zhang Jian Miao Dianhu Wu Ximei Li Jiawei Li Jianxiong Lu Shuangbao Gun Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes Current Issues in Molecular Biology pig adipogenesis glucose transcriptome metabolome fat accumulation |
| title | Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes |
| title_full | Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes |
| title_fullStr | Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes |
| title_full_unstemmed | Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes |
| title_short | Transcriptome and Metabolome Analyses Provide Insight into the Glucose-Induced Adipogenesis in Porcine Adipocytes |
| title_sort | transcriptome and metabolome analyses provide insight into the glucose induced adipogenesis in porcine adipocytes |
| topic | pig adipogenesis glucose transcriptome metabolome fat accumulation |
| url | https://www.mdpi.com/1467-3045/46/3/131 |
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