Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptides
The antihypertensive potential of a land snail by-product hydrolysate (SBH), obtained after an industrial treatment of the raw material, was studied in vitro and in vivo. The ACE inhibitory activity of SBH was characterised by an IC50 value of 23 µg⋅mL−1, which was not affected by in vitro digestion...
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| Format: | Article |
| Language: | English |
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Elsevier
2016-04-01
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| Series: | Journal of Functional Foods |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464616000815 |
| _version_ | 1818957550794571776 |
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| author | Benoit Cudennec Nicolas Violle Gabrielle Chataigné Pascal Drevet Jean-François Bisson Pascal Dhulster Rozenn Ravallec |
| author_facet | Benoit Cudennec Nicolas Violle Gabrielle Chataigné Pascal Drevet Jean-François Bisson Pascal Dhulster Rozenn Ravallec |
| author_sort | Benoit Cudennec |
| collection | DOAJ |
| description | The antihypertensive potential of a land snail by-product hydrolysate (SBH), obtained after an industrial treatment of the raw material, was studied in vitro and in vivo. The ACE inhibitory activity of SBH was characterised by an IC50 value of 23 µg⋅mL−1, which was not affected by in vitro digestion. SBH enhanced the Caco-2 intestinal cell metabolic activity and did not induce any toxicity in Wistar rats. The partial purification of SBH led to the obtainment of an active fraction characterised by an IC50 of 0.007 µg⋅mL−1. The sequences of the 17 most abundant peptides of the fraction were identified by LC/MS/MS analysis. Seven of them (YG, YA, VY, SF, FG, GF and VW) are known ACE inhibitory peptides. Finally, in vivo study on SHR rats showed that SBH significantly reduced systolic blood pressure. SBH represents therefore a new candidate as an ingredient for the design of functional foods against hypertension. |
| first_indexed | 2024-12-20T11:11:38Z |
| format | Article |
| id | doaj.art-ef9cda38d6704a659f1a6cdc1fef6d8f |
| institution | Directory Open Access Journal |
| issn | 1756-4646 |
| language | English |
| last_indexed | 2024-12-20T11:11:38Z |
| publishDate | 2016-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj.art-ef9cda38d6704a659f1a6cdc1fef6d8f2022-12-21T19:42:44ZengElsevierJournal of Functional Foods1756-46462016-04-0122602611Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptidesBenoit Cudennec0Nicolas Violle1Gabrielle Chataigné2Pascal Drevet3Jean-François Bisson4Pascal Dhulster5Rozenn Ravallec6Univ. Lille, EA 7394, USC 1281 – ICV – Institut Charles Viollette, F-59000 Lille, France; Corresponding author. Univ. Lille, EA 7394, USC 1281 – ICV – Institut Charles Viollette, F-59000 Lille, France. Tel : +33 (0)3 20 41 75 66; fax: +33 (0)3 28 76 73 56.ETAP-Lab, Health-Nutrition, F-54500 Vandoeuvre-lès-Nancy, FranceUniv. Lille, EA 7394, USC 1281 – ICV – Institut Charles Viollette, F-59000 Lille, FranceHPE Ingrédients, F-14650 Carpiquet, FranceETAP-Lab, Health-Nutrition, F-54500 Vandoeuvre-lès-Nancy, FranceUniv. Lille, EA 7394, USC 1281 – ICV – Institut Charles Viollette, F-59000 Lille, FranceUniv. Lille, EA 7394, USC 1281 – ICV – Institut Charles Viollette, F-59000 Lille, FranceThe antihypertensive potential of a land snail by-product hydrolysate (SBH), obtained after an industrial treatment of the raw material, was studied in vitro and in vivo. The ACE inhibitory activity of SBH was characterised by an IC50 value of 23 µg⋅mL−1, which was not affected by in vitro digestion. SBH enhanced the Caco-2 intestinal cell metabolic activity and did not induce any toxicity in Wistar rats. The partial purification of SBH led to the obtainment of an active fraction characterised by an IC50 of 0.007 µg⋅mL−1. The sequences of the 17 most abundant peptides of the fraction were identified by LC/MS/MS analysis. Seven of them (YG, YA, VY, SF, FG, GF and VW) are known ACE inhibitory peptides. Finally, in vivo study on SHR rats showed that SBH significantly reduced systolic blood pressure. SBH represents therefore a new candidate as an ingredient for the design of functional foods against hypertension.http://www.sciencedirect.com/science/article/pii/S1756464616000815ACE inhibitory peptidesBy-products upgradingLand snailSHR ratsVWVY |
| spellingShingle | Benoit Cudennec Nicolas Violle Gabrielle Chataigné Pascal Drevet Jean-François Bisson Pascal Dhulster Rozenn Ravallec Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptides Journal of Functional Foods ACE inhibitory peptides By-products upgrading Land snail SHR rats VW VY |
| title | Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptides |
| title_full | Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptides |
| title_fullStr | Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptides |
| title_full_unstemmed | Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptides |
| title_short | Evidence for an antihypertensive effect of a land snail (Helix aspersa) by-product hydrolysate – Identification of involved peptides |
| title_sort | evidence for an antihypertensive effect of a land snail helix aspersa by product hydrolysate identification of involved peptides |
| topic | ACE inhibitory peptides By-products upgrading Land snail SHR rats VW VY |
| url | http://www.sciencedirect.com/science/article/pii/S1756464616000815 |
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