Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays
Aptamers are single-stranded oligonucleotides which can be used as alternative recognition elements for protein detection, because aptamers bind their targets with a high affinity similar to antibodies. Due to the target-induced conformational changes of aptamers, these oligonucleotides can be appli...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/9/4/124 |
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| author | Tracy Kurth Sandra Witt Svenja Bolten Janice-Joy Waniek Carlotta Kortmann Antonina Lavrentieva Thomas Scheper Johanna-Gabriela Walter |
| author_facet | Tracy Kurth Sandra Witt Svenja Bolten Janice-Joy Waniek Carlotta Kortmann Antonina Lavrentieva Thomas Scheper Johanna-Gabriela Walter |
| author_sort | Tracy Kurth |
| collection | DOAJ |
| description | Aptamers are single-stranded oligonucleotides which can be used as alternative recognition elements for protein detection, because aptamers bind their targets with a high affinity similar to antibodies. Due to the target-induced conformational changes of aptamers, these oligonucleotides can be applied in various biosensing platforms. In this work, aptamers directed against the vascular endothelial growth factor (VEGF) were used as a model system. VEGF plays a key role in physiological angiogenesis and vasculogenesis. Furthermore, VEGF is involved in the development and growth of cancer and other diseases like age-related macular degeneration, rheumatoid arthritis, diabetes mellitus, and neurodegenerative disorders. Detecting the protein biomarker VEGF is therefore of great importance for medical research and diagnostics. In this research, VEGF-binding aptamers were investigated for the systematic development of a target-induced dissociation (TID) assay utilizing thermophoresis and microarrays. The established aptamer-microarray allowed for the detection of 0.1 nM of VEGF. Furthermore, the systematic development of the TID method using the VEGF model protein could help to develop further TID assays for the detection of various protein biomarkers. |
| first_indexed | 2024-12-19T19:10:51Z |
| format | Article |
| id | doaj.art-efa0df999ff041a19aa8b03b8c21f7b0 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-12-19T19:10:51Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-efa0df999ff041a19aa8b03b8c21f7b02022-12-21T20:09:19ZengMDPI AGBiosensors2079-63742019-10-019412410.3390/bios9040124bios9040124Development of Aptamer-Based TID Assays Using Thermophoresis and MicroarraysTracy Kurth0Sandra Witt1Svenja Bolten2Janice-Joy Waniek3Carlotta Kortmann4Antonina Lavrentieva5Thomas Scheper6Johanna-Gabriela Walter7Institute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyInstitute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyInstitute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyInstitute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyInstitute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyInstitute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyInstitute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyInstitute of Technical Chemistry, Leibniz University of Hannover, Callinstraße 5, 30167 Hannover, GermanyAptamers are single-stranded oligonucleotides which can be used as alternative recognition elements for protein detection, because aptamers bind their targets with a high affinity similar to antibodies. Due to the target-induced conformational changes of aptamers, these oligonucleotides can be applied in various biosensing platforms. In this work, aptamers directed against the vascular endothelial growth factor (VEGF) were used as a model system. VEGF plays a key role in physiological angiogenesis and vasculogenesis. Furthermore, VEGF is involved in the development and growth of cancer and other diseases like age-related macular degeneration, rheumatoid arthritis, diabetes mellitus, and neurodegenerative disorders. Detecting the protein biomarker VEGF is therefore of great importance for medical research and diagnostics. In this research, VEGF-binding aptamers were investigated for the systematic development of a target-induced dissociation (TID) assay utilizing thermophoresis and microarrays. The established aptamer-microarray allowed for the detection of 0.1 nM of VEGF. Furthermore, the systematic development of the TID method using the VEGF model protein could help to develop further TID assays for the detection of various protein biomarkers.https://www.mdpi.com/2079-6374/9/4/124aptamermicroarrayprotein detectiontarget-induced dissociation (tid)biosensorthermophoresisvascular endothelial growth factor (vegf) |
| spellingShingle | Tracy Kurth Sandra Witt Svenja Bolten Janice-Joy Waniek Carlotta Kortmann Antonina Lavrentieva Thomas Scheper Johanna-Gabriela Walter Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays Biosensors aptamer microarray protein detection target-induced dissociation (tid) biosensor thermophoresis vascular endothelial growth factor (vegf) |
| title | Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays |
| title_full | Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays |
| title_fullStr | Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays |
| title_full_unstemmed | Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays |
| title_short | Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays |
| title_sort | development of aptamer based tid assays using thermophoresis and microarrays |
| topic | aptamer microarray protein detection target-induced dissociation (tid) biosensor thermophoresis vascular endothelial growth factor (vegf) |
| url | https://www.mdpi.com/2079-6374/9/4/124 |
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