Presence and Persistence of Andes Virus RNA in Human Semen
When infecting humans, Andes orthohantavirus (ANDV) may cause a severe disease called hantavirus cardiopulmonary syndrome (HCPS). Following non-specific symptoms, the infection may progress to a syndrome of hemorrhagic fever combined with hyper-acute cardiopulmonary failure. The case fatality rate r...
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-11-01
|
| Series: | Viruses |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4915/15/11/2266 |
| _version_ | 1797457516627492864 |
|---|---|
| author | Roland Züst Rahel Ackermann-Gäumann Nicole Liechti Denise Siegrist Sarah Ryter Jasmine Portmann Nicole Lenz Christian Beuret Roger Koller Cornelia Staehelin Andrea B. Kuenzli Jonas Marschall Sylvia Rothenberger Olivier Engler |
| author_facet | Roland Züst Rahel Ackermann-Gäumann Nicole Liechti Denise Siegrist Sarah Ryter Jasmine Portmann Nicole Lenz Christian Beuret Roger Koller Cornelia Staehelin Andrea B. Kuenzli Jonas Marschall Sylvia Rothenberger Olivier Engler |
| author_sort | Roland Züst |
| collection | DOAJ |
| description | When infecting humans, Andes orthohantavirus (ANDV) may cause a severe disease called hantavirus cardiopulmonary syndrome (HCPS). Following non-specific symptoms, the infection may progress to a syndrome of hemorrhagic fever combined with hyper-acute cardiopulmonary failure. The case fatality rate ranges between 25–40%, depending on the outbreak. In this study, we present the follow-up of a male patient who recovered from HCPS six years ago. We demonstrate that the ANDV genome persists within the reproductive tract for at least 71 months. Genome sequence analysis early and late after infection reveals a low number of mutations (two single nucleotide variants and one deletion), suggesting limited replication activity. We can exclude the integration of the viral genome into the host genome, since the treatment of the specimen with RNAse led to a loss of signal. We demonstrate a long-lasting, strong neutralizing antibody response using pseudovirions expressing the ANDV glycoprotein. Taken together, our results show that ANDV has the potential for sexual transmission. |
| first_indexed | 2024-03-09T16:23:09Z |
| format | Article |
| id | doaj.art-efa2c35a36bc4730ae8bab6c7389ba50 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-09T16:23:09Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-efa2c35a36bc4730ae8bab6c7389ba502023-11-24T15:11:01ZengMDPI AGViruses1999-49152023-11-011511226610.3390/v15112266Presence and Persistence of Andes Virus RNA in Human SemenRoland Züst0Rahel Ackermann-Gäumann1Nicole Liechti2Denise Siegrist3Sarah Ryter4Jasmine Portmann5Nicole Lenz6Christian Beuret7Roger Koller8Cornelia Staehelin9Andrea B. Kuenzli10Jonas Marschall11Sylvia Rothenberger12Olivier Engler13Spiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandADMED Microbiology, 2300 La Chaux-de-Fonds, SwitzerlandSpiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandSpiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandSpiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandSpiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandFood Microbial Systems, Risk Assessment and Mitigation Group, Agroscope, 3097 Bern, SwitzerlandSpiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandInstitute for Infectious Diseases, University of Bern, 3001 Bern, SwitzerlandDepartment of Infectious Diseases, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandDepartment of Infectious Diseases, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandDepartment of Infectious Diseases, Inselspital, Bern University Hospital, University of Bern, 3010 Bern, SwitzerlandSpiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandSpiez Laboratory, Swiss Federal Office for Civil Protection, 3700 Spiez, SwitzerlandWhen infecting humans, Andes orthohantavirus (ANDV) may cause a severe disease called hantavirus cardiopulmonary syndrome (HCPS). Following non-specific symptoms, the infection may progress to a syndrome of hemorrhagic fever combined with hyper-acute cardiopulmonary failure. The case fatality rate ranges between 25–40%, depending on the outbreak. In this study, we present the follow-up of a male patient who recovered from HCPS six years ago. We demonstrate that the ANDV genome persists within the reproductive tract for at least 71 months. Genome sequence analysis early and late after infection reveals a low number of mutations (two single nucleotide variants and one deletion), suggesting limited replication activity. We can exclude the integration of the viral genome into the host genome, since the treatment of the specimen with RNAse led to a loss of signal. We demonstrate a long-lasting, strong neutralizing antibody response using pseudovirions expressing the ANDV glycoprotein. Taken together, our results show that ANDV has the potential for sexual transmission.https://www.mdpi.com/1999-4915/15/11/2266Andes viruspersistencesemenneutralizing antibodies |
| spellingShingle | Roland Züst Rahel Ackermann-Gäumann Nicole Liechti Denise Siegrist Sarah Ryter Jasmine Portmann Nicole Lenz Christian Beuret Roger Koller Cornelia Staehelin Andrea B. Kuenzli Jonas Marschall Sylvia Rothenberger Olivier Engler Presence and Persistence of Andes Virus RNA in Human Semen Viruses Andes virus persistence semen neutralizing antibodies |
| title | Presence and Persistence of Andes Virus RNA in Human Semen |
| title_full | Presence and Persistence of Andes Virus RNA in Human Semen |
| title_fullStr | Presence and Persistence of Andes Virus RNA in Human Semen |
| title_full_unstemmed | Presence and Persistence of Andes Virus RNA in Human Semen |
| title_short | Presence and Persistence of Andes Virus RNA in Human Semen |
| title_sort | presence and persistence of andes virus rna in human semen |
| topic | Andes virus persistence semen neutralizing antibodies |
| url | https://www.mdpi.com/1999-4915/15/11/2266 |
| work_keys_str_mv | AT rolandzust presenceandpersistenceofandesvirusrnainhumansemen AT rahelackermanngaumann presenceandpersistenceofandesvirusrnainhumansemen AT nicoleliechti presenceandpersistenceofandesvirusrnainhumansemen AT denisesiegrist presenceandpersistenceofandesvirusrnainhumansemen AT sarahryter presenceandpersistenceofandesvirusrnainhumansemen AT jasmineportmann presenceandpersistenceofandesvirusrnainhumansemen AT nicolelenz presenceandpersistenceofandesvirusrnainhumansemen AT christianbeuret presenceandpersistenceofandesvirusrnainhumansemen AT rogerkoller presenceandpersistenceofandesvirusrnainhumansemen AT corneliastaehelin presenceandpersistenceofandesvirusrnainhumansemen AT andreabkuenzli presenceandpersistenceofandesvirusrnainhumansemen AT jonasmarschall presenceandpersistenceofandesvirusrnainhumansemen AT sylviarothenberger presenceandpersistenceofandesvirusrnainhumansemen AT olivierengler presenceandpersistenceofandesvirusrnainhumansemen |