Histological Changes Associated with the Graft Union Development in Tomato
Despite the importance of grafting in horticultural crops such as tomato (<i>Solanum lycopersicum</i> L.), the structural changes that occur during the graft establishment are little understood. Using histological techniques, the present work examines the time course of changes on the an...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/11/1479 |
| _version_ | 1827702942093803520 |
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| author | Carlos Frey José Luis Acebes Antonio Encina Rafael Álvarez |
| author_facet | Carlos Frey José Luis Acebes Antonio Encina Rafael Álvarez |
| author_sort | Carlos Frey |
| collection | DOAJ |
| description | Despite the importance of grafting in horticultural crops such as tomato (<i>Solanum lycopersicum</i> L.), the structural changes that occur during the graft establishment are little understood. Using histological techniques, the present work examines the time course of changes on the anatomical structure of the graft junction in functional tomato homografts and compares it to that of heterografts and non-functional grafts. No apparent differences were detected between homo- and heterografts, showing similar tissue development. At 10 days after grafting, the cell walls of the scion and rootstock in the area of the graft junction were thicker than usual. Undifferentiated cells and new vascular tissue emerged from the pre-existing vasculature. Adventitious roots appeared mainly on the scion, arising from the pre-existing vasculature. At 20 days, more pronounced vascular tissue was visible, along with large areas showing vascular connection. At 210 days, vestiges of the changes undergone in graft development were still visible. Generally, non-functional grafts presented layers of necrotic remains and deposition of cell wall material in the cut edges, impeding the suitable scion-rootstock connection. Our results show that accurate changes in pre-existing vasculature and the cell walls of the adhesion line are crucial to the development of functional grafts. |
| first_indexed | 2024-03-10T15:08:34Z |
| format | Article |
| id | doaj.art-efa34e270ec546b3b47066b581f84b3a |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T15:08:34Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-efa34e270ec546b3b47066b581f84b3a2023-11-20T19:37:24ZengMDPI AGPlants2223-77472020-11-01911147910.3390/plants9111479Histological Changes Associated with the Graft Union Development in TomatoCarlos Frey0José Luis Acebes1Antonio Encina2Rafael Álvarez3Departamento de Ingeniería y Ciencias Agrarias, Área de Fisiología Vegetal, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, SpainDepartamento de Ingeniería y Ciencias Agrarias, Área de Fisiología Vegetal, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, SpainDepartamento de Ingeniería y Ciencias Agrarias, Área de Fisiología Vegetal, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, SpainDepartamento de Biología Molecular, Área de Biología Celular, Facultad de Ciencias Biológicas y Ambientales, Universidad de León, 24071 León, SpainDespite the importance of grafting in horticultural crops such as tomato (<i>Solanum lycopersicum</i> L.), the structural changes that occur during the graft establishment are little understood. Using histological techniques, the present work examines the time course of changes on the anatomical structure of the graft junction in functional tomato homografts and compares it to that of heterografts and non-functional grafts. No apparent differences were detected between homo- and heterografts, showing similar tissue development. At 10 days after grafting, the cell walls of the scion and rootstock in the area of the graft junction were thicker than usual. Undifferentiated cells and new vascular tissue emerged from the pre-existing vasculature. Adventitious roots appeared mainly on the scion, arising from the pre-existing vasculature. At 20 days, more pronounced vascular tissue was visible, along with large areas showing vascular connection. At 210 days, vestiges of the changes undergone in graft development were still visible. Generally, non-functional grafts presented layers of necrotic remains and deposition of cell wall material in the cut edges, impeding the suitable scion-rootstock connection. Our results show that accurate changes in pre-existing vasculature and the cell walls of the adhesion line are crucial to the development of functional grafts.https://www.mdpi.com/2223-7747/9/11/1479callusgraftinghistologyrootstockscion<i>Solanum lycopersicum</i> |
| spellingShingle | Carlos Frey José Luis Acebes Antonio Encina Rafael Álvarez Histological Changes Associated with the Graft Union Development in Tomato Plants callus grafting histology rootstock scion <i>Solanum lycopersicum</i> |
| title | Histological Changes Associated with the Graft Union Development in Tomato |
| title_full | Histological Changes Associated with the Graft Union Development in Tomato |
| title_fullStr | Histological Changes Associated with the Graft Union Development in Tomato |
| title_full_unstemmed | Histological Changes Associated with the Graft Union Development in Tomato |
| title_short | Histological Changes Associated with the Graft Union Development in Tomato |
| title_sort | histological changes associated with the graft union development in tomato |
| topic | callus grafting histology rootstock scion <i>Solanum lycopersicum</i> |
| url | https://www.mdpi.com/2223-7747/9/11/1479 |
| work_keys_str_mv | AT carlosfrey histologicalchangesassociatedwiththegraftuniondevelopmentintomato AT joseluisacebes histologicalchangesassociatedwiththegraftuniondevelopmentintomato AT antonioencina histologicalchangesassociatedwiththegraftuniondevelopmentintomato AT rafaelalvarez histologicalchangesassociatedwiththegraftuniondevelopmentintomato |