Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients
A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated serologic assays are needed for contact trac...
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| Format: | Article |
| Language: | English |
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Centers for Disease Control and Prevention
2020-07-01
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| Series: | Emerging Infectious Diseases |
| Subjects: | |
| Online Access: | https://wwwnc.cdc.gov/eid/article/26/7/20-0841_article |
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| author | Nisreen M.A. Okba Marcel A. Müller Wentao Li Chunyan Wang Corine H. GeurtsvanKessel Victor M. Corman Mart M. Lamers Reina S. Sikkema Erwin de Bruin Felicity D. Chandler Yazdan Yazdanpanah Quentin Le Hingrat Diane Descamps Nadhira Houhou-Fidouh Chantal B.E.M. Reusken Berend-Jan Bosch Christian Drosten Marion P.G. Koopmans Bart L. Haagmans |
| author_facet | Nisreen M.A. Okba Marcel A. Müller Wentao Li Chunyan Wang Corine H. GeurtsvanKessel Victor M. Corman Mart M. Lamers Reina S. Sikkema Erwin de Bruin Felicity D. Chandler Yazdan Yazdanpanah Quentin Le Hingrat Diane Descamps Nadhira Houhou-Fidouh Chantal B.E.M. Reusken Berend-Jan Bosch Christian Drosten Marion P.G. Koopmans Bart L. Haagmans |
| author_sort | Nisreen M.A. Okba |
| collection | DOAJ |
| description | A new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated serologic assays are needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed serologic assays for detection of SARS-CoV-2 neutralizing, spike protein–specific, and nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed SARS-CoV-2 infections, other coronaviruses, or other respiratory pathogenic infections, we validated and tested various antigens in different in-house and commercial ELISAs. We demonstrated that most PCR-confirmed SARS-CoV-2–infected persons seroconverted by 2 weeks after disease onset. We found that commercial S1 IgG or IgA ELISAs were of lower specificity, and sensitivity varied between the 2 assays; the IgA ELISA showed higher sensitivity. Overall, the validated assays described can be instrumental for detection of SARS-CoV-2–specific antibodies for diagnostic, seroepidemiologic, and vaccine evaluation studies. |
| first_indexed | 2024-12-23T04:28:52Z |
| format | Article |
| id | doaj.art-efaa47224f7f477981d2304a96d84325 |
| institution | Directory Open Access Journal |
| issn | 1080-6040 1080-6059 |
| language | English |
| last_indexed | 2024-12-23T04:28:52Z |
| publishDate | 2020-07-01 |
| publisher | Centers for Disease Control and Prevention |
| record_format | Article |
| series | Emerging Infectious Diseases |
| spelling | doaj.art-efaa47224f7f477981d2304a96d843252022-12-21T18:00:06ZengCenters for Disease Control and PreventionEmerging Infectious Diseases1080-60401080-60592020-07-012671478148810.3201/eid2607.200841Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease PatientsNisreen M.A. OkbaMarcel A. MüllerWentao LiChunyan WangCorine H. GeurtsvanKesselVictor M. CormanMart M. LamersReina S. SikkemaErwin de BruinFelicity D. ChandlerYazdan YazdanpanahQuentin Le HingratDiane DescampsNadhira Houhou-FidouhChantal B.E.M. ReuskenBerend-Jan BoschChristian DrostenMarion P.G. KoopmansBart L. HaagmansA new coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has recently emerged to cause a human pandemic. Although molecular diagnostic tests were rapidly developed, serologic assays are still lacking, yet urgently needed. Validated serologic assays are needed for contact tracing, identifying the viral reservoir, and epidemiologic studies. We developed serologic assays for detection of SARS-CoV-2 neutralizing, spike protein–specific, and nucleocapsid-specific antibodies. Using serum samples from patients with PCR-confirmed SARS-CoV-2 infections, other coronaviruses, or other respiratory pathogenic infections, we validated and tested various antigens in different in-house and commercial ELISAs. We demonstrated that most PCR-confirmed SARS-CoV-2–infected persons seroconverted by 2 weeks after disease onset. We found that commercial S1 IgG or IgA ELISAs were of lower specificity, and sensitivity varied between the 2 assays; the IgA ELISA showed higher sensitivity. Overall, the validated assays described can be instrumental for detection of SARS-CoV-2–specific antibodies for diagnostic, seroepidemiologic, and vaccine evaluation studies.https://wwwnc.cdc.gov/eid/article/26/7/20-0841_articleSevere acute respiratory syndrome coronavirus 2SARS-CoV-2coronavirusviruses2019 novel coronavirus diseaseCOVID-19 |
| spellingShingle | Nisreen M.A. Okba Marcel A. Müller Wentao Li Chunyan Wang Corine H. GeurtsvanKessel Victor M. Corman Mart M. Lamers Reina S. Sikkema Erwin de Bruin Felicity D. Chandler Yazdan Yazdanpanah Quentin Le Hingrat Diane Descamps Nadhira Houhou-Fidouh Chantal B.E.M. Reusken Berend-Jan Bosch Christian Drosten Marion P.G. Koopmans Bart L. Haagmans Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients Emerging Infectious Diseases Severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 coronavirus viruses 2019 novel coronavirus disease COVID-19 |
| title | Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients |
| title_full | Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients |
| title_fullStr | Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients |
| title_full_unstemmed | Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients |
| title_short | Severe Acute Respiratory Syndrome Coronavirus 2−Specific Antibody Responses in Coronavirus Disease Patients |
| title_sort | severe acute respiratory syndrome coronavirus 2 specific antibody responses in coronavirus disease patients |
| topic | Severe acute respiratory syndrome coronavirus 2 SARS-CoV-2 coronavirus viruses 2019 novel coronavirus disease COVID-19 |
| url | https://wwwnc.cdc.gov/eid/article/26/7/20-0841_article |
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