MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2
MiR-520d-3p has recently been reported to have anti-tumor function in several cancers, including glioma and gastric cancer. However, the biological function and its mechanism of action remain unclear in cervical cancer (CC). In this study, we observed that miR-520d-3p expression was lowly expressed...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844023059972 |
| _version_ | 1797732967313833984 |
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| author | Yuan Zhang Fei Tian Jing Zhao |
| author_facet | Yuan Zhang Fei Tian Jing Zhao |
| author_sort | Yuan Zhang |
| collection | DOAJ |
| description | MiR-520d-3p has recently been reported to have anti-tumor function in several cancers, including glioma and gastric cancer. However, the biological function and its mechanism of action remain unclear in cervical cancer (CC). In this study, we observed that miR-520d-3p expression was lowly expressed in CC specimens compared with adjacent normal specimens using reverse transcription quantitative PCR. Moreover, low miR-520d-3p expression was correlated with FIGO stage and lymph node metastasis by Chi-square test. Functionally, overexpression of miR-520d-3p suppressed the proliferation and migration and invasion of two CC cell lines (HeLa and SiHa) using CCK-8 assay and wound healing assay. After target prediction, luciferase reporter assay showed that zinc finger protein 36 ring finger protein‐like 2 (ZFP36L2) was a direct target of miR-520d-3p in CC cells. The expression levels of ZFP36L2 at protein and mRNA were significantly increased in CC tissues compared with adjacent tissues. The expression of ZFP36L2 was negatively correlated with miR-520d-3p in the patients with CC. Importantly, ZFP36L2 overexpression abolished the effects of miR-520d-3p on cell proliferation, migration and EMT process in CC cells. In conclusion, our findings indicate that targeting miR-520d-3p/ZFP36L2 axis might be a promising therapeutic target for CC treatment. |
| first_indexed | 2024-03-12T12:22:10Z |
| format | Article |
| id | doaj.art-efab991ad811406097ecba6d2b57838f |
| institution | Directory Open Access Journal |
| issn | 2405-8440 |
| language | English |
| last_indexed | 2024-03-12T12:22:10Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj.art-efab991ad811406097ecba6d2b57838f2023-08-30T05:52:38ZengElsevierHeliyon2405-84402023-08-0198e18789MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2Yuan Zhang0Fei Tian1Jing Zhao2Corresponding author. Department of Gynecology, Hebei General Hospital, No. 348 Western Peace Road, Xinhua District, Shijiazhuang, 050051, Hebei, China.; Department of Gynecology, Hebei General Hospital, Shijiazhuang, Hebei, ChinaDepartment of Gynecology, Hebei General Hospital, Shijiazhuang, Hebei, ChinaDepartment of Gynecology, Hebei General Hospital, Shijiazhuang, Hebei, ChinaMiR-520d-3p has recently been reported to have anti-tumor function in several cancers, including glioma and gastric cancer. However, the biological function and its mechanism of action remain unclear in cervical cancer (CC). In this study, we observed that miR-520d-3p expression was lowly expressed in CC specimens compared with adjacent normal specimens using reverse transcription quantitative PCR. Moreover, low miR-520d-3p expression was correlated with FIGO stage and lymph node metastasis by Chi-square test. Functionally, overexpression of miR-520d-3p suppressed the proliferation and migration and invasion of two CC cell lines (HeLa and SiHa) using CCK-8 assay and wound healing assay. After target prediction, luciferase reporter assay showed that zinc finger protein 36 ring finger protein‐like 2 (ZFP36L2) was a direct target of miR-520d-3p in CC cells. The expression levels of ZFP36L2 at protein and mRNA were significantly increased in CC tissues compared with adjacent tissues. The expression of ZFP36L2 was negatively correlated with miR-520d-3p in the patients with CC. Importantly, ZFP36L2 overexpression abolished the effects of miR-520d-3p on cell proliferation, migration and EMT process in CC cells. In conclusion, our findings indicate that targeting miR-520d-3p/ZFP36L2 axis might be a promising therapeutic target for CC treatment.http://www.sciencedirect.com/science/article/pii/S2405844023059972Cervical cancermiR-520d-3pZFP36L2ProliferationEpithelial-mesenchymal transition |
| spellingShingle | Yuan Zhang Fei Tian Jing Zhao MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2 Heliyon Cervical cancer miR-520d-3p ZFP36L2 Proliferation Epithelial-mesenchymal transition |
| title | MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2 |
| title_full | MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2 |
| title_fullStr | MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2 |
| title_full_unstemmed | MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2 |
| title_short | MiR-520d-3p suppresses the proliferation and epithelial-mesenchymal transition of cervical cancer cells by targeting ZFP36L2 |
| title_sort | mir 520d 3p suppresses the proliferation and epithelial mesenchymal transition of cervical cancer cells by targeting zfp36l2 |
| topic | Cervical cancer miR-520d-3p ZFP36L2 Proliferation Epithelial-mesenchymal transition |
| url | http://www.sciencedirect.com/science/article/pii/S2405844023059972 |
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