Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer
Abstract Background Circulating tumor cells (CTCs) play a crucial role in the early diagnosis and prognosis of lung cancer. Identification of a more suitable sample source could be a breakthrough towards enhancing CTC detectability in early‐stage lung cancer. We investigated the differences in detec...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2024-03-01
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| Series: | Thoracic Cancer |
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| Online Access: | https://doi.org/10.1111/1759-7714.15236 |
| _version_ | 1797265497840943104 |
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| author | Zhen‐dan Wang Yi‐fei Feng Yu‐shuo Wang Ying Ma Jiyan Liu Dihua Li Sheng Li Guo‐dong Zhang |
| author_facet | Zhen‐dan Wang Yi‐fei Feng Yu‐shuo Wang Ying Ma Jiyan Liu Dihua Li Sheng Li Guo‐dong Zhang |
| author_sort | Zhen‐dan Wang |
| collection | DOAJ |
| description | Abstract Background Circulating tumor cells (CTCs) play a crucial role in the early diagnosis and prognosis of lung cancer. Identification of a more suitable sample source could be a breakthrough towards enhancing CTC detectability in early‐stage lung cancer. We investigated the differences in detectable CTCs between peripheral arterial and venous blood in early‐ and mid‐stage lung cancer patients undergoing surgery and analyzed the association between clinicopathological factors and detectable CTCs in peripheral arterial and venous blood. Methods Peripheral arterial and venous blood was collected in 5‐mL samples from 56 patients with surgically resected and pathologically clear at early‐ or mid‐stage lung cancer. Blood specimens were enriched for CTCs based on isolation by size of epithelial tumor cells. The CTCs were identified using Swiss Giemsa staining and immunohistochemistry for CD45/CD31. Results In stage I lung cancer, CTC‐positive rate was significantly higher in peripheral arterial than in venous blood (45.45% vs. 17.39%). There was no significant difference in the number of detectable CTCs between peripheral arterial and venous blood. A low degree of differentiation was associated with a high positive rate of CTCs in peripheral venous blood. The number of circulating tumor microemboli was significantly higher in patients with tumor size >3 cm compared with ≤3 cm. Conclusion CTC levels in peripheral arterial and venous blood differed little in lung cancer patients.Compared to peripheral venous blood, peripheral arterial blood had a higher CTC positivity rate in early‐stage lung cancer.This study was favorable for early detection and monitoring of lung cancer. |
| first_indexed | 2024-04-25T00:45:45Z |
| format | Article |
| id | doaj.art-efae3d0ba7ca4be28d41bb0afd3d46ba |
| institution | Directory Open Access Journal |
| issn | 1759-7706 1759-7714 |
| language | English |
| last_indexed | 2024-04-25T00:45:45Z |
| publishDate | 2024-03-01 |
| publisher | Wiley |
| record_format | Article |
| series | Thoracic Cancer |
| spelling | doaj.art-efae3d0ba7ca4be28d41bb0afd3d46ba2024-03-12T05:30:58ZengWileyThoracic Cancer1759-77061759-77142024-03-0115865466010.1111/1759-7714.15236Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancerZhen‐dan Wang0Yi‐fei Feng1Yu‐shuo Wang2Ying Ma3Jiyan Liu4Dihua Li5Sheng Li6Guo‐dong Zhang7Department of Thoracic Surgery Shandong University Cancer Center Jinan ChinaSchool of Clinical and Basic Medicine Shandong First Medical University Jinan ChinaDepartment of Primary Care and Population Health, Institute of Epidemiology and Public Health London's Global University London UKShandong Pharmaceutical Research Institute Jinan ChinaShandong Pharmaceutical Research Institute Jinan ChinaShandong Pharmaceutical Research Institute Jinan ChinaShandong Pharmaceutical Research Institute Jinan ChinaDepartment of Thoracic Surgery, Shandong Cancer Hospital and Institute Shandong First Medical University and Shandong Academy of Medical Sciences Jinan ChinaAbstract Background Circulating tumor cells (CTCs) play a crucial role in the early diagnosis and prognosis of lung cancer. Identification of a more suitable sample source could be a breakthrough towards enhancing CTC detectability in early‐stage lung cancer. We investigated the differences in detectable CTCs between peripheral arterial and venous blood in early‐ and mid‐stage lung cancer patients undergoing surgery and analyzed the association between clinicopathological factors and detectable CTCs in peripheral arterial and venous blood. Methods Peripheral arterial and venous blood was collected in 5‐mL samples from 56 patients with surgically resected and pathologically clear at early‐ or mid‐stage lung cancer. Blood specimens were enriched for CTCs based on isolation by size of epithelial tumor cells. The CTCs were identified using Swiss Giemsa staining and immunohistochemistry for CD45/CD31. Results In stage I lung cancer, CTC‐positive rate was significantly higher in peripheral arterial than in venous blood (45.45% vs. 17.39%). There was no significant difference in the number of detectable CTCs between peripheral arterial and venous blood. A low degree of differentiation was associated with a high positive rate of CTCs in peripheral venous blood. The number of circulating tumor microemboli was significantly higher in patients with tumor size >3 cm compared with ≤3 cm. Conclusion CTC levels in peripheral arterial and venous blood differed little in lung cancer patients.Compared to peripheral venous blood, peripheral arterial blood had a higher CTC positivity rate in early‐stage lung cancer.This study was favorable for early detection and monitoring of lung cancer.https://doi.org/10.1111/1759-7714.15236circulating tumor cellslung cancerperipheral arterial bloodperipheral venous bloodpositivity rate |
| spellingShingle | Zhen‐dan Wang Yi‐fei Feng Yu‐shuo Wang Ying Ma Jiyan Liu Dihua Li Sheng Li Guo‐dong Zhang Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer Thoracic Cancer circulating tumor cells lung cancer peripheral arterial blood peripheral venous blood positivity rate |
| title | Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer |
| title_full | Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer |
| title_fullStr | Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer |
| title_full_unstemmed | Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer |
| title_short | Peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer |
| title_sort | peripheral arterial rather than venous blood is a better source of circulating tumor cells in early lung cancer |
| topic | circulating tumor cells lung cancer peripheral arterial blood peripheral venous blood positivity rate |
| url | https://doi.org/10.1111/1759-7714.15236 |
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