Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum
Cyanobacterial metabolites are natural products that have an important feature in pharmaceutical and medicinal industries. In this study, the presence of the secondary metabolite norharmane in the indole structure was determined in Geitlerinema carotinosum (Geitler) Anagnostidis isolated from Tokat...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
International Journal of Secondary Metabolite
2017-11-01
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| Series: | International Journal of Secondary Metabolite |
| Subjects: | |
| Online Access: | https://dergipark.org.tr/tr/pub/ijsm/issue/32131/356247?publisher=ijate |
| _version_ | 1797923575238230016 |
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| author | Tunay Karan Ramazan Erenler |
| author_facet | Tunay Karan Ramazan Erenler |
| author_sort | Tunay Karan |
| collection | DOAJ |
| description | Cyanobacterial
metabolites are natural products that have an important feature in
pharmaceutical and medicinal industries. In this study, the presence of the
secondary metabolite norharmane in the indole structure was determined in Geitlerinema carotinosum (Geitler)
Anagnostidis isolated from Tokat Yesilirmak River and its production in salt
stress and pH stress was investigated. In salt stress experiments,
cyanobacterium was cultured for two weeks by adding NaCl to BG11 medium in
erlenmayers of 0.5, 1.0, 3.0, 5.0
M. pH stress
was executed at 5 and 9. Norharmane amount was determined by HPLC using
C18 reverse phase column at a temperature of 40 °C and a flow rate of 1 ml /
min. The amount of norharman metabolite (μg/g) was calculated according to the
Gauss method by drawing a calibration curve over the absorbance value of the
standard 247 nm wavelength. According to the analysis results, metabolite
production was 0.612, 1.299, 0.011 at 0.5 M, 1.0 M, 3.0 M respectively. At 5 M,
there was no norharmane production. The
norharmane production is higher at pH 5 (1.293 μg /g) than that of the pH 9 (0.448 μg /g). |
| first_indexed | 2024-04-10T14:48:31Z |
| format | Article |
| id | doaj.art-efb1966394e84411bc6593bdb19be772 |
| institution | Directory Open Access Journal |
| issn | 2148-6905 |
| language | English |
| last_indexed | 2024-04-10T14:48:31Z |
| publishDate | 2017-11-01 |
| publisher | International Journal of Secondary Metabolite |
| record_format | Article |
| series | International Journal of Secondary Metabolite |
| spelling | doaj.art-efb1966394e84411bc6593bdb19be7722023-02-15T16:07:42ZengInternational Journal of Secondary MetaboliteInternational Journal of Secondary Metabolite2148-69052017-11-0143, Special Issue 1161910.21448/ijsm.356247618Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosumTunay KaranRamazan ErenlerCyanobacterial metabolites are natural products that have an important feature in pharmaceutical and medicinal industries. In this study, the presence of the secondary metabolite norharmane in the indole structure was determined in Geitlerinema carotinosum (Geitler) Anagnostidis isolated from Tokat Yesilirmak River and its production in salt stress and pH stress was investigated. In salt stress experiments, cyanobacterium was cultured for two weeks by adding NaCl to BG11 medium in erlenmayers of 0.5, 1.0, 3.0, 5.0 M. pH stress was executed at 5 and 9. Norharmane amount was determined by HPLC using C18 reverse phase column at a temperature of 40 °C and a flow rate of 1 ml / min. The amount of norharman metabolite (μg/g) was calculated according to the Gauss method by drawing a calibration curve over the absorbance value of the standard 247 nm wavelength. According to the analysis results, metabolite production was 0.612, 1.299, 0.011 at 0.5 M, 1.0 M, 3.0 M respectively. At 5 M, there was no norharmane production. The norharmane production is higher at pH 5 (1.293 μg /g) than that of the pH 9 (0.448 μg /g).https://dergipark.org.tr/tr/pub/ijsm/issue/32131/356247?publisher=ijategeitlerinema carotinosumstress conditionsnorharmanehplc |
| spellingShingle | Tunay Karan Ramazan Erenler Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum International Journal of Secondary Metabolite geitlerinema carotinosum stress conditions norharmane hplc |
| title | Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum |
| title_full | Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum |
| title_fullStr | Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum |
| title_full_unstemmed | Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum |
| title_short | Effect of Salt and pH Stress of Bioactive Metabolite Production in Geitlerinema carotinosum |
| title_sort | effect of salt and ph stress of bioactive metabolite production in geitlerinema carotinosum |
| topic | geitlerinema carotinosum stress conditions norharmane hplc |
| url | https://dergipark.org.tr/tr/pub/ijsm/issue/32131/356247?publisher=ijate |
| work_keys_str_mv | AT tunaykaran effectofsaltandphstressofbioactivemetaboliteproductioningeitlerinemacarotinosum AT ramazanerenler effectofsaltandphstressofbioactivemetaboliteproductioningeitlerinemacarotinosum |