Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity
Premna integrifolia L. is widely distributed in tropical and subtropical regions. In the present study, green silver nanoparticles were synthesized efficiently after mixing 1 mmol/L AgNO3 and 4% aqueous leaf extract at neutral pH (7.0) after 25 min sunlight exposure. The aqueous leaf extract was enr...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2019.1577699 |
| _version_ | 1818067329048641536 |
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| author | Chandrashekhar Singh Jitendra Kumar Pradeep Kumar Brijesh Singh Chauhan Kavindra Nath Tiwari Sunil Kumar Mishra S. Srikrishna Rajesh Saini Gopal Nath Jasmeet Singh |
| author_facet | Chandrashekhar Singh Jitendra Kumar Pradeep Kumar Brijesh Singh Chauhan Kavindra Nath Tiwari Sunil Kumar Mishra S. Srikrishna Rajesh Saini Gopal Nath Jasmeet Singh |
| author_sort | Chandrashekhar Singh |
| collection | DOAJ |
| description | Premna integrifolia L. is widely distributed in tropical and subtropical regions. In the present study, green silver nanoparticles were synthesized efficiently after mixing 1 mmol/L AgNO3 and 4% aqueous leaf extract at neutral pH (7.0) after 25 min sunlight exposure. The aqueous leaf extract was enriched with polyphenols. It had higher flavonoid (67.23 ± 1.23 µg/mg gallic acid equivalent) than phenolic content (58.10 ± 2.29 µg/mg rutin equivalent). The bio-synthesized silver nanoparticles were characterized by different spectroscopic and microscopic studies. The synthesized nanoparticles were spherical in shape and ranged from 9 to 35 nm in size. The crystalline nature of the nanoparticles was confirmed on the basis of high-resolution transmission electron microscopy (HRTEM), high-resolution scanning electron microscopy (HRSEM), X-ray diffractometry (XRD) and selected area electron diffraction (SAED) analyses. The presence of silver ions in the biosynthesized nanoparticles was demonstrated based on energy-dispersive X-ray (EDX) data (3.5 keV). The functional groups involved in the nanoparticle synthesis were analysed using FT-IR. These silver nanoparticles showed good antibacterial activity against human pathogenic gram-positive (Staphylococcus aureus, Enterococcus faecalis) and gram-negative (Shigella dysenteriae, Shigella flexneri and Vibrio parahaemolyticus) bacteria. The silver nanoparticles exhibited good in vitro antioxidant and cytotoxic activity to human cervical cancer cell line (SiHa). |
| first_indexed | 2024-12-10T15:21:57Z |
| format | Article |
| id | doaj.art-efb1d82991b04d51a187290e7b0a64d2 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-10T15:21:57Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-efb1d82991b04d51a187290e7b0a64d22022-12-22T01:43:39ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-0133135937110.1080/13102818.2019.15776991577699Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activityChandrashekhar Singh0Jitendra Kumar1Pradeep Kumar2Brijesh Singh Chauhan3Kavindra Nath Tiwari4Sunil Kumar Mishra5S. Srikrishna6Rajesh Saini7Gopal Nath8Jasmeet Singh9Banaras Hindu UniversityIndian Institute of Technology, Banaras Hindu UniversityBanaras Hindu UniversityInstitute of Science, Banaras Hindu UniversityBanaras Hindu UniversityIndian Institute of Technology, Banaras Hindu UniversityInstitute of Science, Banaras Hindu UniversityBanaras Hindu UniversityInstitute of Medical Sciences, Banaras Hindu UniversityInstitute of Medical Sciences, Banaras Hindu UniversityPremna integrifolia L. is widely distributed in tropical and subtropical regions. In the present study, green silver nanoparticles were synthesized efficiently after mixing 1 mmol/L AgNO3 and 4% aqueous leaf extract at neutral pH (7.0) after 25 min sunlight exposure. The aqueous leaf extract was enriched with polyphenols. It had higher flavonoid (67.23 ± 1.23 µg/mg gallic acid equivalent) than phenolic content (58.10 ± 2.29 µg/mg rutin equivalent). The bio-synthesized silver nanoparticles were characterized by different spectroscopic and microscopic studies. The synthesized nanoparticles were spherical in shape and ranged from 9 to 35 nm in size. The crystalline nature of the nanoparticles was confirmed on the basis of high-resolution transmission electron microscopy (HRTEM), high-resolution scanning electron microscopy (HRSEM), X-ray diffractometry (XRD) and selected area electron diffraction (SAED) analyses. The presence of silver ions in the biosynthesized nanoparticles was demonstrated based on energy-dispersive X-ray (EDX) data (3.5 keV). The functional groups involved in the nanoparticle synthesis were analysed using FT-IR. These silver nanoparticles showed good antibacterial activity against human pathogenic gram-positive (Staphylococcus aureus, Enterococcus faecalis) and gram-negative (Shigella dysenteriae, Shigella flexneri and Vibrio parahaemolyticus) bacteria. The silver nanoparticles exhibited good in vitro antioxidant and cytotoxic activity to human cervical cancer cell line (SiHa).http://dx.doi.org/10.1080/13102818.2019.1577699premna integrifolia; silver nanoparticlesantimicrobialcytotoxicityantioxidants |
| spellingShingle | Chandrashekhar Singh Jitendra Kumar Pradeep Kumar Brijesh Singh Chauhan Kavindra Nath Tiwari Sunil Kumar Mishra S. Srikrishna Rajesh Saini Gopal Nath Jasmeet Singh Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity Biotechnology & Biotechnological Equipment premna integrifolia; silver nanoparticles antimicrobial cytotoxicity antioxidants |
| title | Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity |
| title_full | Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity |
| title_fullStr | Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity |
| title_full_unstemmed | Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity |
| title_short | Green synthesis of silver nanoparticles using aqueous leaf extract of Premna integrifolia (L.) rich in polyphenols and evaluation of their antioxidant, antibacterial and cytotoxic activity |
| title_sort | green synthesis of silver nanoparticles using aqueous leaf extract of premna integrifolia l rich in polyphenols and evaluation of their antioxidant antibacterial and cytotoxic activity |
| topic | premna integrifolia; silver nanoparticles antimicrobial cytotoxicity antioxidants |
| url | http://dx.doi.org/10.1080/13102818.2019.1577699 |
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