The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn
Summary: Some Zinc finger (ZnF) proteins are required for masculinization in silkworms. In the present study, a masculinizer gene (Mr-Masc) with multi-tissue expression is identified in the freshwater prawn Macrobrachium rosenbergii. The Mr-Masc is clustered into a separate branch with ZnF proteins...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-07-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2589004223010453 |
| _version_ | 1797774583989796864 |
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| author | Jing-Yu Luo Shuai-Qi Shen Hai-Jing Xu Jin-Shu Yang Wen-Ming Ma |
| author_facet | Jing-Yu Luo Shuai-Qi Shen Hai-Jing Xu Jin-Shu Yang Wen-Ming Ma |
| author_sort | Jing-Yu Luo |
| collection | DOAJ |
| description | Summary: Some Zinc finger (ZnF) proteins are required for masculinization in silkworms. In the present study, a masculinizer gene (Mr-Masc) with multi-tissue expression is identified in the freshwater prawn Macrobrachium rosenbergii. The Mr-Masc is clustered into a separate branch with ZnF proteins from decapoda by phylogenetic tree analysis. Moreover, Mr-Masc silencing in male postlarvae prawn results in functional sex reversal females known as neo-females, which are applied to all-male monosex offspring breeding. This manipulation has been significant in sexually dimorphic cultured species. In addition, several significantly expressed transcripts are enriched and the effects of crucial signal pathways are focused through the comparative transcriptomic analysis in Mr-Masc gene knockdown. The significantly differentially expressed epidermal growth factor, upregulated low-density lipoprotein receptor, flotillin, and sex-lethal unigenes, downregulated heat shock proteins and forkhead box homologs are focused. The finding offers an innovative perspective on Masc proteins’ evolution and physiological function. |
| first_indexed | 2024-03-12T22:23:13Z |
| format | Article |
| id | doaj.art-efb3339fe7194250a798dcda834db973 |
| institution | Directory Open Access Journal |
| issn | 2589-0042 |
| language | English |
| last_indexed | 2024-03-12T22:23:13Z |
| publishDate | 2023-07-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj.art-efb3339fe7194250a798dcda834db9732023-07-23T04:55:00ZengElsevieriScience2589-00422023-07-01267106968The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawnJing-Yu Luo0Shuai-Qi Shen1Hai-Jing Xu2Jin-Shu Yang3Wen-Ming Ma4College of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, People’s Republic of ChinaCollege of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, People’s Republic of China; College of Life Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, People’s Republic of ChinaCollege of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, People’s Republic of ChinaCollege of Life Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, People’s Republic of ChinaCollege of Biological and Environmental Sciences, Zhejiang Wanli University, Ningbo, Zhejiang 315100, People’s Republic of China; Corresponding authorSummary: Some Zinc finger (ZnF) proteins are required for masculinization in silkworms. In the present study, a masculinizer gene (Mr-Masc) with multi-tissue expression is identified in the freshwater prawn Macrobrachium rosenbergii. The Mr-Masc is clustered into a separate branch with ZnF proteins from decapoda by phylogenetic tree analysis. Moreover, Mr-Masc silencing in male postlarvae prawn results in functional sex reversal females known as neo-females, which are applied to all-male monosex offspring breeding. This manipulation has been significant in sexually dimorphic cultured species. In addition, several significantly expressed transcripts are enriched and the effects of crucial signal pathways are focused through the comparative transcriptomic analysis in Mr-Masc gene knockdown. The significantly differentially expressed epidermal growth factor, upregulated low-density lipoprotein receptor, flotillin, and sex-lethal unigenes, downregulated heat shock proteins and forkhead box homologs are focused. The finding offers an innovative perspective on Masc proteins’ evolution and physiological function.http://www.sciencedirect.com/science/article/pii/S2589004223010453Evolutionary biologyGeneticsZoology |
| spellingShingle | Jing-Yu Luo Shuai-Qi Shen Hai-Jing Xu Jin-Shu Yang Wen-Ming Ma The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn iScience Evolutionary biology Genetics Zoology |
| title | The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn |
| title_full | The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn |
| title_fullStr | The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn |
| title_full_unstemmed | The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn |
| title_short | The transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn |
| title_sort | transcription factor masculinizer in sexual differentiation and achieved full functional sex reversal in prawn |
| topic | Evolutionary biology Genetics Zoology |
| url | http://www.sciencedirect.com/science/article/pii/S2589004223010453 |
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