Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco
Abstract Background Salt stress causes inhibition of plant growth and development, and always leads to an increasing threat to plant agriculture. Transcription factors regulate the expression of various genes for stress response and adaptation. It’s crucial to reveal the regulatory mechanisms of tra...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2022-05-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-022-03623-8 |
| _version_ | 1811254275310878720 |
|---|---|
| author | Xuemei Zhang Zihan Cheng Gaofeng Fan Wenjing Yao Wei Li Sixue Chen Tingbo Jiang |
| author_facet | Xuemei Zhang Zihan Cheng Gaofeng Fan Wenjing Yao Wei Li Sixue Chen Tingbo Jiang |
| author_sort | Xuemei Zhang |
| collection | DOAJ |
| description | Abstract Background Salt stress causes inhibition of plant growth and development, and always leads to an increasing threat to plant agriculture. Transcription factors regulate the expression of various genes for stress response and adaptation. It’s crucial to reveal the regulatory mechanisms of transcription factors in the response to salt stress. Results A salt-inducible NAC transcription factor gene PagNAC045 was isolated from Populus alba×P. glandulosa. The PagNAC045 had a high sequence similarity with NAC045 (Potri.007G099400.1) in P. trichocarpa, and they both contained the same conserved motifs 1 and 2, which constitute the highly conserved NAM domain at the N-terminus. Protein-protein interaction (PPI) prediction showed that PagNAC045 potentially interacts with many proteins involved in plant hormone signaling, DNA-binding and transcriptional regulation. The results of subcellular localization and transient expression in tobacco leaves confirmed the nuclear localization of PagNAC045. Yeast two-hybrid revealed that PagNAC045 protein exhibits transcriptional activation property and the activation domain located in its C-terminus. In addition, the 1063 bp promoter of PagNAC045 was able to drive GUS gene expression in the leaves and roots. In poplar leaves and roots, PagNAC045 expression increased significantly by salt and ABA treatments. Tobacco seedlings overexpressing PagNAC045 exhibited enhanced tolerance to NaCl and ABA compared to the wild-type (WT). Yeast one-hybrid assay demonstrated that a bHLH104-like transcription factor can bind to the promoter sequence of PagNAC045. Conclusion The PagNAC045 functions as positive regulator in plant responses to NaCl and ABA-mediated stresses. |
| first_indexed | 2024-04-12T17:04:51Z |
| format | Article |
| id | doaj.art-efb55e516f9e4d72903daef33cbe7744 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-04-12T17:04:51Z |
| publishDate | 2022-05-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-efb55e516f9e4d72903daef33cbe77442022-12-22T03:23:59ZengBMCBMC Plant Biology1471-22292022-05-0122111410.1186/s12870-022-03623-8Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobaccoXuemei Zhang0Zihan Cheng1Gaofeng Fan2Wenjing Yao3Wei Li4Sixue Chen5Tingbo Jiang6State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityState Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityDepartment of Biology, Genetics Institute, University of Florida (UF)State Key Laboratory of Tree Genetics and Breeding, Northeast Forestry UniversityAbstract Background Salt stress causes inhibition of plant growth and development, and always leads to an increasing threat to plant agriculture. Transcription factors regulate the expression of various genes for stress response and adaptation. It’s crucial to reveal the regulatory mechanisms of transcription factors in the response to salt stress. Results A salt-inducible NAC transcription factor gene PagNAC045 was isolated from Populus alba×P. glandulosa. The PagNAC045 had a high sequence similarity with NAC045 (Potri.007G099400.1) in P. trichocarpa, and they both contained the same conserved motifs 1 and 2, which constitute the highly conserved NAM domain at the N-terminus. Protein-protein interaction (PPI) prediction showed that PagNAC045 potentially interacts with many proteins involved in plant hormone signaling, DNA-binding and transcriptional regulation. The results of subcellular localization and transient expression in tobacco leaves confirmed the nuclear localization of PagNAC045. Yeast two-hybrid revealed that PagNAC045 protein exhibits transcriptional activation property and the activation domain located in its C-terminus. In addition, the 1063 bp promoter of PagNAC045 was able to drive GUS gene expression in the leaves and roots. In poplar leaves and roots, PagNAC045 expression increased significantly by salt and ABA treatments. Tobacco seedlings overexpressing PagNAC045 exhibited enhanced tolerance to NaCl and ABA compared to the wild-type (WT). Yeast one-hybrid assay demonstrated that a bHLH104-like transcription factor can bind to the promoter sequence of PagNAC045. Conclusion The PagNAC045 functions as positive regulator in plant responses to NaCl and ABA-mediated stresses.https://doi.org/10.1186/s12870-022-03623-8Populus alba×P. glandulosaPagNAC045Transcription factorTobaccoSalt stressABA treatment |
| spellingShingle | Xuemei Zhang Zihan Cheng Gaofeng Fan Wenjing Yao Wei Li Sixue Chen Tingbo Jiang Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco BMC Plant Biology Populus alba×P. glandulosa PagNAC045 Transcription factor Tobacco Salt stress ABA treatment |
| title | Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco |
| title_full | Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco |
| title_fullStr | Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco |
| title_full_unstemmed | Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco |
| title_short | Functional analysis of PagNAC045 transcription factor that improves salt and ABA tolerance in transgenic tobacco |
| title_sort | functional analysis of pagnac045 transcription factor that improves salt and aba tolerance in transgenic tobacco |
| topic | Populus alba×P. glandulosa PagNAC045 Transcription factor Tobacco Salt stress ABA treatment |
| url | https://doi.org/10.1186/s12870-022-03623-8 |
| work_keys_str_mv | AT xuemeizhang functionalanalysisofpagnac045transcriptionfactorthatimprovessaltandabatoleranceintransgenictobacco AT zihancheng functionalanalysisofpagnac045transcriptionfactorthatimprovessaltandabatoleranceintransgenictobacco AT gaofengfan functionalanalysisofpagnac045transcriptionfactorthatimprovessaltandabatoleranceintransgenictobacco AT wenjingyao functionalanalysisofpagnac045transcriptionfactorthatimprovessaltandabatoleranceintransgenictobacco AT weili functionalanalysisofpagnac045transcriptionfactorthatimprovessaltandabatoleranceintransgenictobacco AT sixuechen functionalanalysisofpagnac045transcriptionfactorthatimprovessaltandabatoleranceintransgenictobacco AT tingbojiang functionalanalysisofpagnac045transcriptionfactorthatimprovessaltandabatoleranceintransgenictobacco |