Impact of Sunitinib on Human Thyroid Cancer Cells
Background/Aims: Thyroid cancer accounts for about 1% of all cancer cases. Multikinase inhibitors like sunitinib (S) have a promising potential in thyroid cancer therapy. Therefore, the principal aim of this study was to investigate the impact of sunitinib on the secretion of cytokines of follicular...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Cell Physiol Biochem Press GmbH & Co KG
2013-07-01
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| Series: | Cellular Physiology and Biochemistry |
| Subjects: | |
| Online Access: | http://www.karger.com/Article/FullText/350132 |
| _version_ | 1819039101902389248 |
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| author | Jirka Grosse Elisabeth Warnke Fabian Pohl Nils E. Magnusson Markus Wehland Manfred Infanger Christoph Eilles Daniela Grimm |
| author_facet | Jirka Grosse Elisabeth Warnke Fabian Pohl Nils E. Magnusson Markus Wehland Manfred Infanger Christoph Eilles Daniela Grimm |
| author_sort | Jirka Grosse |
| collection | DOAJ |
| description | Background/Aims: Thyroid cancer accounts for about 1% of all cancer cases. Multikinase inhibitors like sunitinib (S) have a promising potential in thyroid cancer therapy. Therefore, the principal aim of this study was to investigate the impact of sunitinib on the secretion of cytokines of follicular thyroid cancer cells. Method: The effects of irradiation (R), S, and their combination (R+S) on cytokine secretion by the human thyroid cancer cell lines ML-1 and CGTH W-1 were evaluated after two (d2) and four days (d4) of treatment. Results: Multi-Analyte Profiling of cytokine release showed a decrease after S treatment (CGTH W-1: IFN-γ, IL-4, IL-8 d2, MIP-1a, MMP-2, TNF-α and TNF-β; ML-1: IFN-γ, IL-4, IL-6, IL-7, IL-8; MIP-1α, MMP-2, MCP-1, TNF-α and TNF-β). R elevated significantly the release of cytokines (exception ML-1: MCP-1, MMP-2; CGTH W-1: IL-4, TNF-β). In contrast, R+S treatment resulted in a reduction of IFN-γ, IL-4, and MMP-2 in both cell lines. IL-6, IL-8 and MCP-1 proteins in the supernatant correlated with the data obtained by quantitative RT-PCR. VEGFD mRNAs were significantly elevated by R+S. Conclusion: A target-based therapy with R+S changed VEGFD, IL-6 and IL-8 in follicular thyroid cancer cells. These in vitro-experiments suggest IL-6, IL-8, VEGFD and TNF-α as interesting biomarkers to be investigated in vivo. Different reactions of the cell lines under equal treatment might be due to their different origin and characteristics. |
| first_indexed | 2024-12-21T08:47:52Z |
| format | Article |
| id | doaj.art-efb9278c31124f348ed1ae044c1c7502 |
| institution | Directory Open Access Journal |
| issn | 1015-8987 1421-9778 |
| language | English |
| last_indexed | 2024-12-21T08:47:52Z |
| publishDate | 2013-07-01 |
| publisher | Cell Physiol Biochem Press GmbH & Co KG |
| record_format | Article |
| series | Cellular Physiology and Biochemistry |
| spelling | doaj.art-efb9278c31124f348ed1ae044c1c75022022-12-21T19:09:46ZengCell Physiol Biochem Press GmbH & Co KGCellular Physiology and Biochemistry1015-89871421-97782013-07-0132115417010.1159/000350132350132Impact of Sunitinib on Human Thyroid Cancer CellsJirka GrosseElisabeth WarnkeFabian PohlNils E. MagnussonMarkus WehlandManfred InfangerChristoph EillesDaniela GrimmBackground/Aims: Thyroid cancer accounts for about 1% of all cancer cases. Multikinase inhibitors like sunitinib (S) have a promising potential in thyroid cancer therapy. Therefore, the principal aim of this study was to investigate the impact of sunitinib on the secretion of cytokines of follicular thyroid cancer cells. Method: The effects of irradiation (R), S, and their combination (R+S) on cytokine secretion by the human thyroid cancer cell lines ML-1 and CGTH W-1 were evaluated after two (d2) and four days (d4) of treatment. Results: Multi-Analyte Profiling of cytokine release showed a decrease after S treatment (CGTH W-1: IFN-γ, IL-4, IL-8 d2, MIP-1a, MMP-2, TNF-α and TNF-β; ML-1: IFN-γ, IL-4, IL-6, IL-7, IL-8; MIP-1α, MMP-2, MCP-1, TNF-α and TNF-β). R elevated significantly the release of cytokines (exception ML-1: MCP-1, MMP-2; CGTH W-1: IL-4, TNF-β). In contrast, R+S treatment resulted in a reduction of IFN-γ, IL-4, and MMP-2 in both cell lines. IL-6, IL-8 and MCP-1 proteins in the supernatant correlated with the data obtained by quantitative RT-PCR. VEGFD mRNAs were significantly elevated by R+S. Conclusion: A target-based therapy with R+S changed VEGFD, IL-6 and IL-8 in follicular thyroid cancer cells. These in vitro-experiments suggest IL-6, IL-8, VEGFD and TNF-α as interesting biomarkers to be investigated in vivo. Different reactions of the cell lines under equal treatment might be due to their different origin and characteristics.http://www.karger.com/Article/FullText/350132Thyroid cancerSunitinibCytokinesRadiationVEGFGene expression |
| spellingShingle | Jirka Grosse Elisabeth Warnke Fabian Pohl Nils E. Magnusson Markus Wehland Manfred Infanger Christoph Eilles Daniela Grimm Impact of Sunitinib on Human Thyroid Cancer Cells Cellular Physiology and Biochemistry Thyroid cancer Sunitinib Cytokines Radiation VEGF Gene expression |
| title | Impact of Sunitinib on Human Thyroid Cancer Cells |
| title_full | Impact of Sunitinib on Human Thyroid Cancer Cells |
| title_fullStr | Impact of Sunitinib on Human Thyroid Cancer Cells |
| title_full_unstemmed | Impact of Sunitinib on Human Thyroid Cancer Cells |
| title_short | Impact of Sunitinib on Human Thyroid Cancer Cells |
| title_sort | impact of sunitinib on human thyroid cancer cells |
| topic | Thyroid cancer Sunitinib Cytokines Radiation VEGF Gene expression |
| url | http://www.karger.com/Article/FullText/350132 |
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