Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection
Abstract Rubus glaucus Benth (known as “mora de castilla”) is a Colombian agricultural product, with probably, the major potential. This fruit combines features of Idaeobatus and Rubus subgenera. Despite its recognized importance in the economy of small producers, this crop has received little techn...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Fruticultura
2019-01-01
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| Series: | Revista Brasileira de Fruticultura |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000100803&lng=en&tlng=en |
| _version_ | 1811323786579935232 |
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| author | Ana María López Carlos Felipe Barrera Marta Leonor Marulanda |
| author_facet | Ana María López Carlos Felipe Barrera Marta Leonor Marulanda |
| author_sort | Ana María López |
| collection | DOAJ |
| description | Abstract Rubus glaucus Benth (known as “mora de castilla”) is a Colombian agricultural product, with probably, the major potential. This fruit combines features of Idaeobatus and Rubus subgenera. Despite its recognized importance in the economy of small producers, this crop has received little technological development; as a result, sowing procedures of this specie is done by using local cultivars asexually propagated by producers. Associated Rubus producers in Colombia has noticed the necessity to formalize the offer of planting material, starting with plant breeding programs tending to the obtention of more productive varieties with morphological features that facilitates cultural activities. This study presents the results of the evaluation of SSR and SNPs markers obtained in previous experimental works from a RNA-Seq transcriptome analysis. It was evaluated 15 promissory R. glaucus cultivars, which could be potential progenitors in future breeding schemes. Genetic characterization was accomplished by testing 22 SSR microsatellite and 78 single nucleotide polymorphisms (SNPs) markers. From evaluated SSR markers, 15 yielded positive PCR amplification generating 29 loci and 58 alleles. From evaluated SNPs markers, 36 yielded positive PCR amplification. Obtained sequences from amplified products with SNPs showed high homology with species belonging to Rosaceae family. Selection criteria of progenitors were based on the results of molecular characterizations and useful morphological features in the culture management. This research demonstrates the utility of molecular markers to assess genetic diversity of potential progenitors susceptible to plant breeding processes. This process, highly known as development of parental populations, determines in a great manner the success of plant breeding processes. |
| first_indexed | 2024-04-13T14:02:41Z |
| format | Article |
| id | doaj.art-efb972a7400f40e1a3d9115404951e23 |
| institution | Directory Open Access Journal |
| issn | 1806-9967 |
| language | English |
| last_indexed | 2024-04-13T14:02:41Z |
| publishDate | 2019-01-01 |
| publisher | Sociedade Brasileira de Fruticultura |
| record_format | Article |
| series | Revista Brasileira de Fruticultura |
| spelling | doaj.art-efb972a7400f40e1a3d9115404951e232022-12-22T02:44:01ZengSociedade Brasileira de FruticulturaRevista Brasileira de Fruticultura1806-99672019-01-0141110.1590/0100-29452019081S0100-29452019000100803Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selectionAna María LópezCarlos Felipe BarreraMarta Leonor MarulandaAbstract Rubus glaucus Benth (known as “mora de castilla”) is a Colombian agricultural product, with probably, the major potential. This fruit combines features of Idaeobatus and Rubus subgenera. Despite its recognized importance in the economy of small producers, this crop has received little technological development; as a result, sowing procedures of this specie is done by using local cultivars asexually propagated by producers. Associated Rubus producers in Colombia has noticed the necessity to formalize the offer of planting material, starting with plant breeding programs tending to the obtention of more productive varieties with morphological features that facilitates cultural activities. This study presents the results of the evaluation of SSR and SNPs markers obtained in previous experimental works from a RNA-Seq transcriptome analysis. It was evaluated 15 promissory R. glaucus cultivars, which could be potential progenitors in future breeding schemes. Genetic characterization was accomplished by testing 22 SSR microsatellite and 78 single nucleotide polymorphisms (SNPs) markers. From evaluated SSR markers, 15 yielded positive PCR amplification generating 29 loci and 58 alleles. From evaluated SNPs markers, 36 yielded positive PCR amplification. Obtained sequences from amplified products with SNPs showed high homology with species belonging to Rosaceae family. Selection criteria of progenitors were based on the results of molecular characterizations and useful morphological features in the culture management. This research demonstrates the utility of molecular markers to assess genetic diversity of potential progenitors susceptible to plant breeding processes. This process, highly known as development of parental populations, determines in a great manner the success of plant breeding processes.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000100803&lng=en&tlng=enFito-melhoramentoamora de castilladiversidade genética |
| spellingShingle | Ana María López Carlos Felipe Barrera Marta Leonor Marulanda Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection Revista Brasileira de Fruticultura Fito-melhoramento amora de castilla diversidade genética |
| title | Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection |
| title_full | Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection |
| title_fullStr | Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection |
| title_full_unstemmed | Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection |
| title_short | Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection |
| title_sort | evaluation of ssr and snp markers in rubus glaucus benth progenitors selection |
| topic | Fito-melhoramento amora de castilla diversidade genética |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000100803&lng=en&tlng=en |
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