An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth
Legumes play a crucial role in the restoration and utilization of salinized grassland. To explore the physiological response mechanism of Astragalus membranaceus and Medicago sativa seedlings to salt stress, salt stress culture experiments with five NaCl concentration treatments (0 mmol/L, 50 mmol/L...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-01-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fpls.2023.1342219/full |
| _version_ | 1797347922742870016 |
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| author | Jia Mi Jia Mi Jia Mi Xinyue Ren Jing Shi Fei Wang Qianju Wang Haiyan Pang Lifang Kang Changhui Wang Changhui Wang Changhui Wang |
| author_facet | Jia Mi Jia Mi Jia Mi Xinyue Ren Jing Shi Fei Wang Qianju Wang Haiyan Pang Lifang Kang Changhui Wang Changhui Wang Changhui Wang |
| author_sort | Jia Mi |
| collection | DOAJ |
| description | Legumes play a crucial role in the restoration and utilization of salinized grassland. To explore the physiological response mechanism of Astragalus membranaceus and Medicago sativa seedlings to salt stress, salt stress culture experiments with five NaCl concentration treatments (0 mmol/L, 50 mmol/L, 100 mmol/L, 200 mmol/L, and 300 mmol/L) were conducted on these two legume seedlings. Morphological characteristics, physiological features, biomass, and the protective enzyme system were measured for both seedlings. Correlation analysis, principal component analysis (PCA), and membership function analysis (MFA) were conducted for each index. Structural equation modeling (SEM) was employed to analyze the salt stress pathways of plants. The results indicated that number of primary branches (PBN), ascorbate peroxidase (APX) activity in stems and leaves, catalase (CAT) activity in roots, etc. were identified as the primary indicators for evaluating the salt tolerance of A. membranaceus during its seedling growth period. And CAT and peroxidase (POD) activity in roots, POD and superoxide dismutase (SOD) activity in stems and leaves, etc. were identified as the primary indicators for evaluating the salt tolerance of M. sativa during its growth period. Plant morphological characteristics, physiological indexes, and underground biomass (UGB) were directly affected by salinity, while physiological indexes indirectly affected the degree of leaf succulence (LSD). Regarding the response of the protective enzyme system to salt stress, the activity of POD and APX increased in A. membranaceus, while the activity of CAT increased in M. sativa. Our findings suggest that salt stress directly affects the growth strategies of legumes. Furthermore, the response of the protective enzyme system and potential cell membrane damage to salinity were very different in the two legumes. |
| first_indexed | 2024-03-08T11:55:55Z |
| format | Article |
| id | doaj.art-efbabe3a754645219baf6eff5d1e40b1 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-03-08T11:55:55Z |
| publishDate | 2024-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-efbabe3a754645219baf6eff5d1e40b12024-01-24T04:19:33ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2024-01-011410.3389/fpls.2023.13422191342219An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growthJia Mi0Jia Mi1Jia Mi2Xinyue Ren3Jing Shi4Fei Wang5Qianju Wang6Haiyan Pang7Lifang Kang8Changhui Wang9Changhui Wang10Changhui Wang11Shanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan, ChinaField Scientific Observation and Research Station of the Ministry of Education for Subalpine Grassland Ecosystem in Shanxi, Ningwu, ChinaShanxi Key Laboratory of Grassland Ecological Protection and Native Grass Germplasm Innovation, Shanxi Agricultural University, Taigu, ChinaShanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan, ChinaCollege of Environment and Resources Sciences, Shanxi University, Taiyuan, ChinaShanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan, ChinaShanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan, ChinaShanxi Key Laboratory of Ecological Restoration on Loess Plateau, Institute of Loess Plateau, Shanxi University, Taiyuan, ChinaKey Laboratory of Plant Resources and Beijing Botanical Garden, Institute of Botany, Chinese Academy of Sciences, Beijing, ChinaShanxi Key Laboratory of Grassland Ecological Protection and Native Grass Germplasm Innovation, Shanxi Agricultural University, Taigu, ChinaCollege of Grassland Science, Shanxi Agricultural University, Taigu, ChinaObservation and Research Station for Grassland Ecosystem in the Loess Plateau, Shanxi Agricultural University, Taigu, ChinaLegumes play a crucial role in the restoration and utilization of salinized grassland. To explore the physiological response mechanism of Astragalus membranaceus and Medicago sativa seedlings to salt stress, salt stress culture experiments with five NaCl concentration treatments (0 mmol/L, 50 mmol/L, 100 mmol/L, 200 mmol/L, and 300 mmol/L) were conducted on these two legume seedlings. Morphological characteristics, physiological features, biomass, and the protective enzyme system were measured for both seedlings. Correlation analysis, principal component analysis (PCA), and membership function analysis (MFA) were conducted for each index. Structural equation modeling (SEM) was employed to analyze the salt stress pathways of plants. The results indicated that number of primary branches (PBN), ascorbate peroxidase (APX) activity in stems and leaves, catalase (CAT) activity in roots, etc. were identified as the primary indicators for evaluating the salt tolerance of A. membranaceus during its seedling growth period. And CAT and peroxidase (POD) activity in roots, POD and superoxide dismutase (SOD) activity in stems and leaves, etc. were identified as the primary indicators for evaluating the salt tolerance of M. sativa during its growth period. Plant morphological characteristics, physiological indexes, and underground biomass (UGB) were directly affected by salinity, while physiological indexes indirectly affected the degree of leaf succulence (LSD). Regarding the response of the protective enzyme system to salt stress, the activity of POD and APX increased in A. membranaceus, while the activity of CAT increased in M. sativa. Our findings suggest that salt stress directly affects the growth strategies of legumes. Furthermore, the response of the protective enzyme system and potential cell membrane damage to salinity were very different in the two legumes.https://www.frontiersin.org/articles/10.3389/fpls.2023.1342219/fullsalt stresslegumeseedlingmorphological and physiological characteristicsprotective enzyme systemstructural equation modeling |
| spellingShingle | Jia Mi Jia Mi Jia Mi Xinyue Ren Jing Shi Fei Wang Qianju Wang Haiyan Pang Lifang Kang Changhui Wang Changhui Wang Changhui Wang An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth Frontiers in Plant Science salt stress legume seedling morphological and physiological characteristics protective enzyme system structural equation modeling |
| title | An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth |
| title_full | An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth |
| title_fullStr | An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth |
| title_full_unstemmed | An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth |
| title_short | An insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth |
| title_sort | insight into the different responses to salt stress in growth characteristics of two legume species during seedling growth |
| topic | salt stress legume seedling morphological and physiological characteristics protective enzyme system structural equation modeling |
| url | https://www.frontiersin.org/articles/10.3389/fpls.2023.1342219/full |
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