Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases
In this study, symptomatic fish samples were taken from rainbow trout farms. Isolation and identification of agents isolated from fish samples were made. DNA isolations from different purified colonies were carried out with the mericon bacterial DNA kit. Real-Time PCR procedure was performed by usin...
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| Format: | Article |
| Language: | English |
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Yuzuncu Yil University Faculty of Agriculture
2021-06-01
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| Series: | Yüzüncü Yil Üniversitesi Tarim Bilimleri Dergisi |
| Subjects: |
| _version_ | 1797723371929075712 |
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| author | Şükrü Önalan Aslı ÇİLİNGİR YELTEKİN |
| author_facet | Şükrü Önalan Aslı ÇİLİNGİR YELTEKİN |
| author_sort | Şükrü Önalan |
| collection | DOAJ |
| description | In this study, symptomatic fish samples were taken from rainbow trout farms. Isolation and identification of agents isolated from fish samples were made. DNA isolations from different purified colonies were carried out with the mericon bacterial DNA kit. Real-Time PCR procedure was performed by using universal bacterial primers. Molecular identifications were performed by blasting the nucleotides obtained by sequence analysis of PCR amplicons. Spectrophotometric measurements were performed at 412 nm wavelengths for AChE activity and 412 nm for BChE activity from liver and brain tissues of fish samples. The activity differences of different disease factors among themselves and according to the control group were examined. As a result of the study, isolation and identification of Bacillus subtilis, Lactococcus garvieae and Staphylococcus epidermidis from 5 different farms were performed. Over 98% similarity was observed as a result of sequencing analysis of the isolates. In this study, it was observed that three different bacteria isolated from trout farms suppressed AChE and BChE enzyme activities in both tissues of trout. |
| first_indexed | 2024-03-12T10:01:56Z |
| format | Article |
| id | doaj.art-efbd8d7633a54047981fafccb77466ef |
| institution | Directory Open Access Journal |
| issn | 1308-7576 1308-7584 |
| language | English |
| last_indexed | 2024-03-12T10:01:56Z |
| publishDate | 2021-06-01 |
| publisher | Yuzuncu Yil University Faculty of Agriculture |
| record_format | Article |
| series | Yüzüncü Yil Üniversitesi Tarim Bilimleri Dergisi |
| spelling | doaj.art-efbd8d7633a54047981fafccb77466ef2023-09-02T11:39:23ZengYuzuncu Yil University Faculty of AgricultureYüzüncü Yil Üniversitesi Tarim Bilimleri Dergisi1308-75761308-75842021-06-0131235636410.29133/yyutbd.853386Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases Şükrü Önalanhttps://orcid.org/0000-0003-0058-5232Aslı ÇİLİNGİR YELTEKİNhttps://orcid.org/0000-0003-0071-7434In this study, symptomatic fish samples were taken from rainbow trout farms. Isolation and identification of agents isolated from fish samples were made. DNA isolations from different purified colonies were carried out with the mericon bacterial DNA kit. Real-Time PCR procedure was performed by using universal bacterial primers. Molecular identifications were performed by blasting the nucleotides obtained by sequence analysis of PCR amplicons. Spectrophotometric measurements were performed at 412 nm wavelengths for AChE activity and 412 nm for BChE activity from liver and brain tissues of fish samples. The activity differences of different disease factors among themselves and according to the control group were examined. As a result of the study, isolation and identification of Bacillus subtilis, Lactococcus garvieae and Staphylococcus epidermidis from 5 different farms were performed. Over 98% similarity was observed as a result of sequencing analysis of the isolates. In this study, it was observed that three different bacteria isolated from trout farms suppressed AChE and BChE enzyme activities in both tissues of trout.achebacteriabchesequencingrainbow troutreal-time pcr |
| spellingShingle | Şükrü Önalan Aslı ÇİLİNGİR YELTEKİN Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases Yüzüncü Yil Üniversitesi Tarim Bilimleri Dergisi ache bacteria bche sequencing rainbow trout real-time pcr |
| title | Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases |
| title_full | Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases |
| title_fullStr | Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases |
| title_full_unstemmed | Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases |
| title_short | Differences in AChE and BChE Enzyme Activation Levels in Liver and Brain Tissues in Rainbow Trouts Exposed to Different Bacterial Diseases |
| title_sort | differences in ache and bche enzyme activation levels in liver and brain tissues in rainbow trouts exposed to different bacterial diseases |
| topic | ache bacteria bche sequencing rainbow trout real-time pcr |
| work_keys_str_mv | AT sukruonalan differencesinacheandbcheenzymeactivationlevelsinliverandbraintissuesinrainbowtroutsexposedtodifferentbacterialdiseases AT aslıcilingiryeltekin differencesinacheandbcheenzymeactivationlevelsinliverandbraintissuesinrainbowtroutsexposedtodifferentbacterialdiseases |