A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i>
Antimicrobial resistance has become one of the major global public health concerns, and it is indispensable to search for alternatives to conventional antibiotics. Recently, antimicrobial peptides have received great attention because of their broad-spectrum antimicrobial activity at relatively low...
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| Format: | Article |
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MDPI AG
2021-06-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/6/1372 |
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| author | Rossella Festa Rosa Luisa Ambrosio Alexandre Lamas Lorena Gratino Gianna Palmieri Carlos Manuel Franco Alberto Cepeda Aniello Anastasio |
| author_facet | Rossella Festa Rosa Luisa Ambrosio Alexandre Lamas Lorena Gratino Gianna Palmieri Carlos Manuel Franco Alberto Cepeda Aniello Anastasio |
| author_sort | Rossella Festa |
| collection | DOAJ |
| description | Antimicrobial resistance has become one of the major global public health concerns, and it is indispensable to search for alternatives to conventional antibiotics. Recently, antimicrobial peptides have received great attention because of their broad-spectrum antimicrobial activity at relatively low concentrations, even against pathogens such as <i>Salmonella enterica</i>, which is responsible for most food-borne illnesses. This work aimed at evaluating the antimicrobial and antibiofilm activity of the innate defense peptide, named 1018-K6, against <i>S. enterica</i>. A total of 42 strains, belonging to three different subspecies and 32 serotypes, were included in this study. The antibiotic resistance profile of all the strains and the cytotoxic effects of 1018-K6 on mammalian fibroblast cells were also investigated. Results revealed that MIC (minimum inhibitory concentrations) and MBC (minimum bactericidal concentrations) values were in the ranges of 8–64 μg/mL and 16–128 μg/mL, respectively, although most strains (97%) showed MICs between 16 and 32 μg/mL. Moreover, sub-inhibitory concentrations of 1018-K6 strongly reduced the biofilm formation in several <i>S. enterica</i> strains, whatever the initial inoculum size. Our results demonstrated that 1018-K6 is able to control and manage <i>S. enterica</i> growth with a large potential for applications in the fields of active packaging and water disinfectants. |
| first_indexed | 2024-03-10T10:25:53Z |
| format | Article |
| id | doaj.art-efbd9da92a084a98bba008b5d690bea4 |
| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-10T10:25:53Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-efbd9da92a084a98bba008b5d690bea42023-11-22T00:01:39ZengMDPI AGFoods2304-81582021-06-01106137210.3390/foods10061372A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i>Rossella Festa0Rosa Luisa Ambrosio1Alexandre Lamas2Lorena Gratino3Gianna Palmieri4Carlos Manuel Franco5Alberto Cepeda6Aniello Anastasio7Department of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, ItalyLaboratorio de Higiene Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, 27002 Lugo, SpainInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), 80131 Napoli, ItalyInstitute of Biosciences and BioResources, National Research Council (IBBR-CNR), 80131 Napoli, ItalyLaboratorio de Higiene Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, 27002 Lugo, SpainLaboratorio de Higiene Inspección y Control de Alimentos, Departamento de Química Analítica, Nutrición y Bromatología, Universidad de Santiago de Compostela, 27002 Lugo, SpainDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, 80137 Napoli, ItalyAntimicrobial resistance has become one of the major global public health concerns, and it is indispensable to search for alternatives to conventional antibiotics. Recently, antimicrobial peptides have received great attention because of their broad-spectrum antimicrobial activity at relatively low concentrations, even against pathogens such as <i>Salmonella enterica</i>, which is responsible for most food-borne illnesses. This work aimed at evaluating the antimicrobial and antibiofilm activity of the innate defense peptide, named 1018-K6, against <i>S. enterica</i>. A total of 42 strains, belonging to three different subspecies and 32 serotypes, were included in this study. The antibiotic resistance profile of all the strains and the cytotoxic effects of 1018-K6 on mammalian fibroblast cells were also investigated. Results revealed that MIC (minimum inhibitory concentrations) and MBC (minimum bactericidal concentrations) values were in the ranges of 8–64 μg/mL and 16–128 μg/mL, respectively, although most strains (97%) showed MICs between 16 and 32 μg/mL. Moreover, sub-inhibitory concentrations of 1018-K6 strongly reduced the biofilm formation in several <i>S. enterica</i> strains, whatever the initial inoculum size. Our results demonstrated that 1018-K6 is able to control and manage <i>S. enterica</i> growth with a large potential for applications in the fields of active packaging and water disinfectants.https://www.mdpi.com/2304-8158/10/6/1372antimicrobial peptide<i>Salmonella</i>food-borne pathogenbiofilm1018-k16food preservatives |
| spellingShingle | Rossella Festa Rosa Luisa Ambrosio Alexandre Lamas Lorena Gratino Gianna Palmieri Carlos Manuel Franco Alberto Cepeda Aniello Anastasio A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i> Foods antimicrobial peptide <i>Salmonella</i> food-borne pathogen biofilm 1018-k16 food preservatives |
| title | A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i> |
| title_full | A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i> |
| title_fullStr | A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i> |
| title_full_unstemmed | A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i> |
| title_short | A Study on the Antimicrobial and Antibiofilm Peptide 1018-K6 as Potential Alternative to Antibiotics against Food-Pathogen <i>Salmonella enterica</i> |
| title_sort | study on the antimicrobial and antibiofilm peptide 1018 k6 as potential alternative to antibiotics against food pathogen i salmonella enterica i |
| topic | antimicrobial peptide <i>Salmonella</i> food-borne pathogen biofilm 1018-k16 food preservatives |
| url | https://www.mdpi.com/2304-8158/10/6/1372 |
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