Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager
Abstract Monitoring of tissue O2 is essential for cancer development and treatment, as hypoxic tumour regions develop resistance to radio- and chemotherapy. We describe a minimally invasive technique for the monitoring of tissue oxygenation in developing grafted tumours, which uses the new phosphore...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2023-10-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-46224-7 |
| _version_ | 1797637012249903104 |
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| author | Alexander V. Zhdanov Rajannya Sen Ciaran Devoy Liang Li Mark Tangney Dmitri B. Papkovsky |
| author_facet | Alexander V. Zhdanov Rajannya Sen Ciaran Devoy Liang Li Mark Tangney Dmitri B. Papkovsky |
| author_sort | Alexander V. Zhdanov |
| collection | DOAJ |
| description | Abstract Monitoring of tissue O2 is essential for cancer development and treatment, as hypoxic tumour regions develop resistance to radio- and chemotherapy. We describe a minimally invasive technique for the monitoring of tissue oxygenation in developing grafted tumours, which uses the new phosphorescence lifetime based Tpx3Cam imager. CT26 cells stained with a near-infrared emitting nanoparticulate O2 probe NanO2-IR were injected into mice to produce grafted tumours with characteristic phosphorescence. The tumours were allowed to develop for 3, 7, 10 and 17 days, with O2 imaging experiments performed on live and euthanised animals at different time points. Despite a marked trend towards decreased O2 in dead animals, their tumour areas produced phosphorescence lifetime values between 44 and 47 µs, which corresponded to hypoxic tissue with 5–20 μM O2. After the O2 imaging in animals, confocal Phosphorescence Lifetime Imaging Microscopy was conducted to examine the distribution of NanO2-IR probe in the tumours, which were excised, fixed and sliced for the purpose. The probe remained visible as bright and discrete ‘islands’ embedded in the tumour tissue until day 17 of tumour growth. Overall, this O2 macro-imaging method using NanO2-IR holds promise for long-term studies with grafted tumours in live animal models, providing quantitative 2D mapping of tissue O2. |
| first_indexed | 2024-03-11T12:42:15Z |
| format | Article |
| id | doaj.art-efbdf3b6216943759e4a3178c8d4b119 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-11T12:42:15Z |
| publishDate | 2023-10-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-efbdf3b6216943759e4a3178c8d4b1192023-11-05T12:15:14ZengNature PortfolioScientific Reports2045-23222023-10-0113111010.1038/s41598-023-46224-7Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imagerAlexander V. Zhdanov0Rajannya Sen1Ciaran Devoy2Liang Li3Mark Tangney4Dmitri B. Papkovsky5School of Biochemistry and Cell Biology, University College CorkSchool of Biochemistry and Cell Biology, University College CorkCancer Research @UCC, University College CorkSchool of Biochemistry and Cell Biology, University College CorkCancer Research @UCC, University College CorkSchool of Biochemistry and Cell Biology, University College CorkAbstract Monitoring of tissue O2 is essential for cancer development and treatment, as hypoxic tumour regions develop resistance to radio- and chemotherapy. We describe a minimally invasive technique for the monitoring of tissue oxygenation in developing grafted tumours, which uses the new phosphorescence lifetime based Tpx3Cam imager. CT26 cells stained with a near-infrared emitting nanoparticulate O2 probe NanO2-IR were injected into mice to produce grafted tumours with characteristic phosphorescence. The tumours were allowed to develop for 3, 7, 10 and 17 days, with O2 imaging experiments performed on live and euthanised animals at different time points. Despite a marked trend towards decreased O2 in dead animals, their tumour areas produced phosphorescence lifetime values between 44 and 47 µs, which corresponded to hypoxic tissue with 5–20 μM O2. After the O2 imaging in animals, confocal Phosphorescence Lifetime Imaging Microscopy was conducted to examine the distribution of NanO2-IR probe in the tumours, which were excised, fixed and sliced for the purpose. The probe remained visible as bright and discrete ‘islands’ embedded in the tumour tissue until day 17 of tumour growth. Overall, this O2 macro-imaging method using NanO2-IR holds promise for long-term studies with grafted tumours in live animal models, providing quantitative 2D mapping of tissue O2.https://doi.org/10.1038/s41598-023-46224-7 |
| spellingShingle | Alexander V. Zhdanov Rajannya Sen Ciaran Devoy Liang Li Mark Tangney Dmitri B. Papkovsky Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager Scientific Reports |
| title | Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager |
| title_full | Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager |
| title_fullStr | Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager |
| title_full_unstemmed | Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager |
| title_short | Analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro-imager |
| title_sort | analysis of tumour oxygenation in model animals on a phosphorescence lifetime based macro imager |
| url | https://doi.org/10.1038/s41598-023-46224-7 |
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