Impact of pasteurization on the self-assembly of human milk lipids during digestion
Human milk is critical for the survival and development of infants. This source of nutrition contains components that protect against infections while stimulating immune maturation. In cases where the mother's own milk is unavailable, pasteurized donor milk is the preferred option. Although pas...
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| Format: | Article |
| Language: | English |
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Elsevier
2022-05-01
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| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227522000165 |
| _version_ | 1811232569695404032 |
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| author | Syaza Y. Binte Abu Bakar Malinda Salim Andrew J. Clulow Adrian Hawley Joseph Pelle Donna T. Geddes Kevin R. Nicholas Ben J. Boyd |
| author_facet | Syaza Y. Binte Abu Bakar Malinda Salim Andrew J. Clulow Adrian Hawley Joseph Pelle Donna T. Geddes Kevin R. Nicholas Ben J. Boyd |
| author_sort | Syaza Y. Binte Abu Bakar |
| collection | DOAJ |
| description | Human milk is critical for the survival and development of infants. This source of nutrition contains components that protect against infections while stimulating immune maturation. In cases where the mother's own milk is unavailable, pasteurized donor milk is the preferred option. Although pasteurization has been shown to have minimal impact on the lipid and FA composition before digestion, no correlation has been made between the impact of pasteurization on the FFA composition and the self-assembly of lipids during digestion, which could act as delivery mechanisms for poorly water-soluble components. Pooled nonpasteurized and pasteurized human milk from a single donor was used in this study. The evolving FFA composition during digestion was determined using GC coupled to a flame ionization detector. In vitro digestion coupled to small-angle X-ray scattering was utilized to investigate the influence of different calcium levels, fat content, and the presence of bile salts on the extent of digestion and structural behavior of human milk lipids. Almost complete digestion was achieved when bile salts were added to the systems containing high calcium to milk fat ratio, with similar structural behavior of lipids during digestion of both types of human milk being apparent. In contrast, differences in the colloidal structures were formed during digestion in the absence of bile salt because of a greater amount of FFAs being released from the nonpasteurized than pasteurized milks. This difference in FFAs released from both types of human milk could result in varying nutritional implications for infants. |
| first_indexed | 2024-04-12T11:05:07Z |
| format | Article |
| id | doaj.art-efbea2d5907e40b7b96e13257ed2dd0b |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-04-12T11:05:07Z |
| publishDate | 2022-05-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-efbea2d5907e40b7b96e13257ed2dd0b2022-12-22T03:35:49ZengElsevierJournal of Lipid Research0022-22752022-05-01635100183Impact of pasteurization on the self-assembly of human milk lipids during digestionSyaza Y. Binte Abu Bakar0Malinda Salim1Andrew J. Clulow2Adrian Hawley3Joseph Pelle4Donna T. Geddes5Kevin R. Nicholas6Ben J. Boyd7Drug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, AustraliaDrug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, AustraliaDrug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia; Australian Synchrotron, ANSTO, Clayton, Victoria, AustraliaAustralian Synchrotron, ANSTO, Clayton, Victoria, AustraliaDrug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, AustraliaSchool of Molecular Science, The University of Western Australia, Crawley, Western Australia, AustraliaDrug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, AustraliaDrug Delivery, Disposition and Dynamics, Monash Institute of Pharmaceutical Sciences, Monash University, Parkville, Victoria, Australia; Department of Pharmacy, University of Copenhagen, København Ø, Denmark; For correspondence: Ben J. BoydHuman milk is critical for the survival and development of infants. This source of nutrition contains components that protect against infections while stimulating immune maturation. In cases where the mother's own milk is unavailable, pasteurized donor milk is the preferred option. Although pasteurization has been shown to have minimal impact on the lipid and FA composition before digestion, no correlation has been made between the impact of pasteurization on the FFA composition and the self-assembly of lipids during digestion, which could act as delivery mechanisms for poorly water-soluble components. Pooled nonpasteurized and pasteurized human milk from a single donor was used in this study. The evolving FFA composition during digestion was determined using GC coupled to a flame ionization detector. In vitro digestion coupled to small-angle X-ray scattering was utilized to investigate the influence of different calcium levels, fat content, and the presence of bile salts on the extent of digestion and structural behavior of human milk lipids. Almost complete digestion was achieved when bile salts were added to the systems containing high calcium to milk fat ratio, with similar structural behavior of lipids during digestion of both types of human milk being apparent. In contrast, differences in the colloidal structures were formed during digestion in the absence of bile salt because of a greater amount of FFAs being released from the nonpasteurized than pasteurized milks. This difference in FFAs released from both types of human milk could result in varying nutritional implications for infants.http://www.sciencedirect.com/science/article/pii/S0022227522000165human breast milklipidsdigestiondevelopmentnutritioncalcium levels |
| spellingShingle | Syaza Y. Binte Abu Bakar Malinda Salim Andrew J. Clulow Adrian Hawley Joseph Pelle Donna T. Geddes Kevin R. Nicholas Ben J. Boyd Impact of pasteurization on the self-assembly of human milk lipids during digestion Journal of Lipid Research human breast milk lipids digestion development nutrition calcium levels |
| title | Impact of pasteurization on the self-assembly of human milk lipids during digestion |
| title_full | Impact of pasteurization on the self-assembly of human milk lipids during digestion |
| title_fullStr | Impact of pasteurization on the self-assembly of human milk lipids during digestion |
| title_full_unstemmed | Impact of pasteurization on the self-assembly of human milk lipids during digestion |
| title_short | Impact of pasteurization on the self-assembly of human milk lipids during digestion |
| title_sort | impact of pasteurization on the self assembly of human milk lipids during digestion |
| topic | human breast milk lipids digestion development nutrition calcium levels |
| url | http://www.sciencedirect.com/science/article/pii/S0022227522000165 |
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