Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil
Abstract This study analyzes the genomic findings of the first report of Salmonella isolate carrying the bla CTX-M-55 gene, recovered from a bacteremic patient from Brazil. A bacterial isolate positive for the bla CTX-M-55 gene was submitted to antimicrobial susceptibility testing by disk diffusion...
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| Format: | Article |
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Nature Portfolio
2023-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-29599-5 |
| _version_ | 1828035295477497856 |
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| author | Amanda Maria de Jesus Bertani Thais Vieira Alex Domingos Reis Carla Adriana dos Santos Elisabete Aparecida de Almeida Carlos Henrique Camargo Monique Ribeiro Tiba Casas |
| author_facet | Amanda Maria de Jesus Bertani Thais Vieira Alex Domingos Reis Carla Adriana dos Santos Elisabete Aparecida de Almeida Carlos Henrique Camargo Monique Ribeiro Tiba Casas |
| author_sort | Amanda Maria de Jesus Bertani |
| collection | DOAJ |
| description | Abstract This study analyzes the genomic findings of the first report of Salmonella isolate carrying the bla CTX-M-55 gene, recovered from a bacteremic patient from Brazil. A bacterial isolate positive for the bla CTX-M-55 gene was submitted to antimicrobial susceptibility testing by disk diffusion and epsilometric test. Whole genome sequencing was performed using Illumina technology. Conjugation assay was performed; plasmid sizes determined by S1-PFGE and plasmid content were investigated by hybrid assembly after MinION long reads sequencing. Isolate 288_18 was identified as sequence type ST13, resistant to ampicillin, cefotaxime, ceftazidime, cefepime, ceftriaxone, and aztreonam. A transferable IncFII plasmid sized approximately 67 kb was found to carry the bla TEM-1 and bla CTX-M-55 in a module consisting of IS26-bla TEM-1B -WbuC-bla CTX-M-55-IS26. In addition, an 117 kb IncI1plasmid was also identified in the 288_18 isolate, but without additional resistance genes. To the best of our knowledge, this is the first report of bla CTX-M-55 in Salmonella isolated from human infection in Brazil. The occurrence of bla CTX-M-55 in the IncFII epidemic plasmid in a relevant clinical human isolate of Salmonella Agona underscores the urgent need for enhanced and effective continuous surveillance for controlling its dissemination. |
| first_indexed | 2024-04-10T15:44:46Z |
| format | Article |
| id | doaj.art-efbec60598fa46f1b34e2eecf82ef3c3 |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-10T15:44:46Z |
| publishDate | 2023-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-efbec60598fa46f1b34e2eecf82ef3c32023-02-12T12:11:31ZengNature PortfolioScientific Reports2045-23222023-02-011311610.1038/s41598-023-29599-5Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in BrazilAmanda Maria de Jesus Bertani0Thais Vieira1Alex Domingos Reis2Carla Adriana dos Santos3Elisabete Aparecida de Almeida4Carlos Henrique Camargo5Monique Ribeiro Tiba Casas6Bacteriology Division, Instituto Adolfo LutzBacteriology Division, Instituto Adolfo LutzBacteriology Division, Instituto Adolfo LutzBacteriology Division, Instituto Adolfo LutzBacteriology Division, Instituto Adolfo LutzBacteriology Division, Instituto Adolfo LutzBacteriology Division, Instituto Adolfo LutzAbstract This study analyzes the genomic findings of the first report of Salmonella isolate carrying the bla CTX-M-55 gene, recovered from a bacteremic patient from Brazil. A bacterial isolate positive for the bla CTX-M-55 gene was submitted to antimicrobial susceptibility testing by disk diffusion and epsilometric test. Whole genome sequencing was performed using Illumina technology. Conjugation assay was performed; plasmid sizes determined by S1-PFGE and plasmid content were investigated by hybrid assembly after MinION long reads sequencing. Isolate 288_18 was identified as sequence type ST13, resistant to ampicillin, cefotaxime, ceftazidime, cefepime, ceftriaxone, and aztreonam. A transferable IncFII plasmid sized approximately 67 kb was found to carry the bla TEM-1 and bla CTX-M-55 in a module consisting of IS26-bla TEM-1B -WbuC-bla CTX-M-55-IS26. In addition, an 117 kb IncI1plasmid was also identified in the 288_18 isolate, but without additional resistance genes. To the best of our knowledge, this is the first report of bla CTX-M-55 in Salmonella isolated from human infection in Brazil. The occurrence of bla CTX-M-55 in the IncFII epidemic plasmid in a relevant clinical human isolate of Salmonella Agona underscores the urgent need for enhanced and effective continuous surveillance for controlling its dissemination.https://doi.org/10.1038/s41598-023-29599-5 |
| spellingShingle | Amanda Maria de Jesus Bertani Thais Vieira Alex Domingos Reis Carla Adriana dos Santos Elisabete Aparecida de Almeida Carlos Henrique Camargo Monique Ribeiro Tiba Casas Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil Scientific Reports |
| title | Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil |
| title_full | Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil |
| title_fullStr | Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil |
| title_full_unstemmed | Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil |
| title_short | Whole genome sequence analysis of the first reported isolate of Salmonella Agona carrying blaCTX-M-55 gene in Brazil |
| title_sort | whole genome sequence analysis of the first reported isolate of salmonella agona carrying blactx m 55 gene in brazil |
| url | https://doi.org/10.1038/s41598-023-29599-5 |
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