Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic Applications
With the rapid increase in the use of optogenetics to investigate nervous systems, there is high demand for neural interfaces that can simultaneously perform optical stimulation and electrophysiological recording. However, high-magnitude stimulation artifacts have prevented experiments from being co...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/21/11528 |
| _version_ | 1797512533171503104 |
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| author | Bangbang Guo Ye Fan Minghao Wang Yuhua Cheng Bowen Ji Ying Chen Gaofeng Wang |
| author_facet | Bangbang Guo Ye Fan Minghao Wang Yuhua Cheng Bowen Ji Ying Chen Gaofeng Wang |
| author_sort | Bangbang Guo |
| collection | DOAJ |
| description | With the rapid increase in the use of optogenetics to investigate nervous systems, there is high demand for neural interfaces that can simultaneously perform optical stimulation and electrophysiological recording. However, high-magnitude stimulation artifacts have prevented experiments from being conducted at a desirably high temporal resolution. Here, a flexible polyimide-based neural probe with polyethylene glycol (PEG) packaged optical fiber and Pt-Black/PEDOT-GO (graphene oxide doped poly(3,4-ethylene-dioxythiophene)) modified microelectrodes was developed to reduce the stimulation artifacts that are induced by photoelectrochemical (PEC) and photovoltaic (PV) effects. The advantages of this design include quick and accurate implantation and high-resolution recording capacities. Firstly, electrochemical performance of the modified microelectrodes is significantly improved due to the large specific surface area of the GO layer. Secondly, good mechanical and electrochemical stability of the modified microelectrodes is obtained by using Pt-Black as bonding layer. Lastly, bench noise recordings revealed that PEC noise amplitude of the modified neural probes could be reduced to less than 50 µV and no PV noise was detected when compared to silicon-based neural probes. The results indicate that this device is a promising optogenetic tool for studying local neural circuits. |
| first_indexed | 2024-03-10T06:02:11Z |
| format | Article |
| id | doaj.art-efc14e8170ae43f9856ee1844eece807 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T06:02:11Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-efc14e8170ae43f9856ee1844eece8072023-11-22T20:53:38ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-10-0122211152810.3390/ijms222111528Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic ApplicationsBangbang Guo0Ye Fan1Minghao Wang2Yuhua Cheng3Bowen Ji4Ying Chen5Gaofeng Wang6Wenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, ChinaWenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, ChinaWenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, ChinaWenzhou Institute of Hangzhou Dianzi University, Wenzhou 325038, ChinaThe Unmanned System Research Institute, Northwestern Polytechnical University, Xi’an 710060, ChinaThe Institute of Flexible Electronics Technology of THU, Jiaxing 314000, ChinaMOE Engineering Research Center of Smart Microsensors and Microsystems, School of Electronics and Information, Hangzhou Dianzi University, Hangzhou 310018, ChinaWith the rapid increase in the use of optogenetics to investigate nervous systems, there is high demand for neural interfaces that can simultaneously perform optical stimulation and electrophysiological recording. However, high-magnitude stimulation artifacts have prevented experiments from being conducted at a desirably high temporal resolution. Here, a flexible polyimide-based neural probe with polyethylene glycol (PEG) packaged optical fiber and Pt-Black/PEDOT-GO (graphene oxide doped poly(3,4-ethylene-dioxythiophene)) modified microelectrodes was developed to reduce the stimulation artifacts that are induced by photoelectrochemical (PEC) and photovoltaic (PV) effects. The advantages of this design include quick and accurate implantation and high-resolution recording capacities. Firstly, electrochemical performance of the modified microelectrodes is significantly improved due to the large specific surface area of the GO layer. Secondly, good mechanical and electrochemical stability of the modified microelectrodes is obtained by using Pt-Black as bonding layer. Lastly, bench noise recordings revealed that PEC noise amplitude of the modified neural probes could be reduced to less than 50 µV and no PV noise was detected when compared to silicon-based neural probes. The results indicate that this device is a promising optogenetic tool for studying local neural circuits.https://www.mdpi.com/1422-0067/22/21/11528optogeneticsphotoelectric artifactPt-Black/PEDOT-GOneural recordingoptical stimulation |
| spellingShingle | Bangbang Guo Ye Fan Minghao Wang Yuhua Cheng Bowen Ji Ying Chen Gaofeng Wang Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic Applications International Journal of Molecular Sciences optogenetics photoelectric artifact Pt-Black/PEDOT-GO neural recording optical stimulation |
| title | Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic Applications |
| title_full | Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic Applications |
| title_fullStr | Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic Applications |
| title_full_unstemmed | Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic Applications |
| title_short | Flexible Neural Probes with Electrochemical Modified Microelectrodes for Artifact-Free Optogenetic Applications |
| title_sort | flexible neural probes with electrochemical modified microelectrodes for artifact free optogenetic applications |
| topic | optogenetics photoelectric artifact Pt-Black/PEDOT-GO neural recording optical stimulation |
| url | https://www.mdpi.com/1422-0067/22/21/11528 |
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