Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women
The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimeste...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira de Pesquisa Odontológica
2012-10-01
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| Series: | Brazilian Oral Research |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500011 |
| _version_ | 1818918626578661376 |
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| author | Fernanda Campos Machado Dionéia Evangelista Cesar Amanda Vervloet Dutra Agostinho Assis Cláudio Galuppo Diniz Rosangela Almeida Ribeiro |
| author_facet | Fernanda Campos Machado Dionéia Evangelista Cesar Amanda Vervloet Dutra Agostinho Assis Cláudio Galuppo Diniz Rosangela Almeida Ribeiro |
| author_sort | Fernanda Campos Machado |
| collection | DOAJ |
| description | The aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women. |
| first_indexed | 2024-12-20T00:52:57Z |
| format | Article |
| id | doaj.art-efc50d18e6124fea91fa990277dfb013 |
| institution | Directory Open Access Journal |
| issn | 1806-8324 |
| language | English |
| last_indexed | 2024-12-20T00:52:57Z |
| publishDate | 2012-10-01 |
| publisher | Sociedade Brasileira de Pesquisa Odontológica |
| record_format | Article |
| series | Brazilian Oral Research |
| spelling | doaj.art-efc50d18e6124fea91fa990277dfb0132022-12-21T19:59:12ZengSociedade Brasileira de Pesquisa OdontológicaBrazilian Oral Research1806-83242012-10-0126544344910.1590/S1806-83242012000500011Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant womenFernanda Campos MachadoDionéia Evangelista CesarAmanda Vervloet Dutra Agostinho AssisCláudio Galuppo DinizRosangela Almeida RibeiroThe aim of this study was to use the fluorescence in situ hybridization (FISH) technique to test the hypothesis of qualitative and quantitative differences of 8 periodontopathogens between pregnant and non-pregnant women. This cross-sectional study included 20 pregnant women in their second trimester of pregnancy and 20 non-pregnant women. Probing depth, bleeding on probing, clinical attachment level, and presence of calculus were recorded. Subgingival plaque samples were collected and the FISH technique identified the presence and numbers of Aggregatibacter actinomycetemcomitans, Tannerella forsythia, Campylobacter rectus, Porphyromonas gingivalis, Treponema denticola, Fusobacterium nucleatum, Prevotella intermedia and Prevotella nigrescens. The Mann-Whitney U-test was applied to compare the data between the two groups. The mean age, ethnicity, marital status, education, and economic level in both groups were similar. The clinical parameters showed no significant differences between pregnant and non-pregnant women. The numbers of subgingival periodontopathogens were not found to be significantly different between groups, despite the higher mean counts of P. intermedia in pregnant women. Colonization patterns of the different bacteria most commonly associated with periodontal disease were not different in the subgingival plaque of pregnant and non-pregnant women.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500011PregnancyMicrobiologyBiofilmsIn situ HybridizationFluorescence |
| spellingShingle | Fernanda Campos Machado Dionéia Evangelista Cesar Amanda Vervloet Dutra Agostinho Assis Cláudio Galuppo Diniz Rosangela Almeida Ribeiro Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women Brazilian Oral Research Pregnancy Microbiology Biofilms In situ Hybridization Fluorescence |
| title | Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
| title_full | Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
| title_fullStr | Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
| title_full_unstemmed | Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
| title_short | Detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
| title_sort | detection and enumeration of periodontopathogenic bacteria in subgingival biofilm of pregnant women |
| topic | Pregnancy Microbiology Biofilms In situ Hybridization Fluorescence |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-83242012000500011 |
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