Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli
Crohn’s disease (CD) is a severe chronic immune-mediated granulomatous inflammatory disease of the gastrointestinal tract. The mechanisms of CD pathogenesis remain obscure. Metagenomic analysis of samples from CD patients revealed that several of them have the elevated level of Escherichia coli with...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2020-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2020.01460/full |
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| author | Olga V. Pobeguts Valentina G. Ladygina Daria V. Evsyutina Artem V. Eremeev Aleksandr I. Zubov Daria S. Matyushkina Peter L. Scherbakov Daria V. Rakitina Gleb Y. Fisunov |
| author_facet | Olga V. Pobeguts Valentina G. Ladygina Daria V. Evsyutina Artem V. Eremeev Aleksandr I. Zubov Daria S. Matyushkina Peter L. Scherbakov Daria V. Rakitina Gleb Y. Fisunov |
| author_sort | Olga V. Pobeguts |
| collection | DOAJ |
| description | Crohn’s disease (CD) is a severe chronic immune-mediated granulomatous inflammatory disease of the gastrointestinal tract. The mechanisms of CD pathogenesis remain obscure. Metagenomic analysis of samples from CD patients revealed that several of them have the elevated level of Escherichia coli with adhesive–invasive phenotype (AIEC). Previously, we isolated an E. coli strain CD isolate ZvL2 from a patient with CD, which features AIEC phenotype. Here, we demonstrate that prolonged growth on propionate containing medium stimulates virulent properties of CD isolate ZvL2, while prolonged growth on glucose reduces these properties to levels indistinguishable from laboratory strain K-12 MG1655. Propionate presence also boosts the ability of CD isolate ZvL2 to penetrate and colonize macrophages. The effect of propionate is reversible, re-passaging of CD isolate on M9 medium supplemented with glucose leads to the loss of its virulent properties. Proteome analysis of CD isolate ZvL2 growth in medium supplemented with propionate or glucose revealed that propionate induces expression porins OmpA and OmpW, transcription factors PhoP and OmpR, and universal stress protein UspE, which were previously found to be important for macrophage colonization by enteropathogenic bacteria. |
| first_indexed | 2024-12-20T19:40:41Z |
| format | Article |
| id | doaj.art-efc5a061723341f79186763a4190d20b |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-20T19:40:41Z |
| publishDate | 2020-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-efc5a061723341f79186763a4190d20b2022-12-21T19:28:32ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2020-07-011110.3389/fmicb.2020.01460515591Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coliOlga V. Pobeguts0Valentina G. Ladygina1Daria V. Evsyutina2Artem V. Eremeev3Aleksandr I. Zubov4Daria S. Matyushkina5Peter L. Scherbakov6Daria V. Rakitina7Gleb Y. Fisunov8Department of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaDepartment of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaDepartment of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaDepartment of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaDepartment of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaDepartment of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaA. S. Loginov Moscow Clinical Scientific Center, Moscow, RussiaDepartment of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaDepartment of Molecular Biology and Genetics, Federal Research and Clinical Centre of Physical and Chemical Medicine, Federal Medical-Biological Agency, Moscow, RussiaCrohn’s disease (CD) is a severe chronic immune-mediated granulomatous inflammatory disease of the gastrointestinal tract. The mechanisms of CD pathogenesis remain obscure. Metagenomic analysis of samples from CD patients revealed that several of them have the elevated level of Escherichia coli with adhesive–invasive phenotype (AIEC). Previously, we isolated an E. coli strain CD isolate ZvL2 from a patient with CD, which features AIEC phenotype. Here, we demonstrate that prolonged growth on propionate containing medium stimulates virulent properties of CD isolate ZvL2, while prolonged growth on glucose reduces these properties to levels indistinguishable from laboratory strain K-12 MG1655. Propionate presence also boosts the ability of CD isolate ZvL2 to penetrate and colonize macrophages. The effect of propionate is reversible, re-passaging of CD isolate on M9 medium supplemented with glucose leads to the loss of its virulent properties. Proteome analysis of CD isolate ZvL2 growth in medium supplemented with propionate or glucose revealed that propionate induces expression porins OmpA and OmpW, transcription factors PhoP and OmpR, and universal stress protein UspE, which were previously found to be important for macrophage colonization by enteropathogenic bacteria.https://www.frontiersin.org/article/10.3389/fmicb.2020.01460/fullCrohn’s diseaseCrohn’s disease-associated adherent-invasive Escherichia colipropionic acid2D electrophoresismacrophages |
| spellingShingle | Olga V. Pobeguts Valentina G. Ladygina Daria V. Evsyutina Artem V. Eremeev Aleksandr I. Zubov Daria S. Matyushkina Peter L. Scherbakov Daria V. Rakitina Gleb Y. Fisunov Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli Frontiers in Microbiology Crohn’s disease Crohn’s disease-associated adherent-invasive Escherichia coli propionic acid 2D electrophoresis macrophages |
| title | Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli |
| title_full | Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli |
| title_fullStr | Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli |
| title_full_unstemmed | Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli |
| title_short | Propionate Induces Virulent Properties of Crohn’s Disease-Associated Escherichia coli |
| title_sort | propionate induces virulent properties of crohn s disease associated escherichia coli |
| topic | Crohn’s disease Crohn’s disease-associated adherent-invasive Escherichia coli propionic acid 2D electrophoresis macrophages |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2020.01460/full |
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