Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes
Ex vivo lung perfusion (EVLP) is a preservation method for donor lungs, which keep lungs viable in a physiological environment outside of a body for a short period of time. EVLP is established clinically for lung transplantation. Experimental applications for EVLP are e.g. lung cancer research or me...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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De Gruyter
2019-09-01
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| Series: | Current Directions in Biomedical Engineering |
| Subjects: | |
| Online Access: | https://doi.org/10.1515/cdbme-2019-0074 |
| _version_ | 1797989334758981632 |
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| author | Pongratz Christina Ziegle Jens Boese Axel Friebe Michael Linge Helena Walles Thorsten |
| author_facet | Pongratz Christina Ziegle Jens Boese Axel Friebe Michael Linge Helena Walles Thorsten |
| author_sort | Pongratz Christina |
| collection | DOAJ |
| description | Ex vivo lung perfusion (EVLP) is a preservation method for donor lungs, which keep lungs viable in a physiological environment outside of a body for a short period of time. EVLP is established clinically for lung transplantation. Experimental applications for EVLP are e.g. lung cancer research or medical device development and testing. For preservation, a lung is ventilated artificially in an organ chamber and perfused antegrade through the pulmonary artery. Here we introduce a thermoregulation system for an experimental EVLP system to be used for translational research approaches as well as for training medical staff. To implement physiological culture conditions that are a prerequisite for lung preservation and tissue homeostasis, a thermoregulation is needed to rewarm the explanted lung tissue (storage temperature 4°C). Technically, the EVLP system must be thermally insulated, so loss of caloric is avoided. For monitoring, temperature sensors are integrated within the lung, in the organ chamber and in the afferent perfusate tube, whereby the measured values determine the thermoregulation. Initial tests using thermal packs (cooled to 4-6°C) placed on a heating mat, as a part of the perfusion circuit, showed that the perfusate temperature falls to 34°C, but restores after approximately 60 minutes (36.5°C), whereby the thermal pack is warmed. With this setup longer perfusion times should be obtained rather than without thermoregulation due to normothermic perfusion of the lung. |
| first_indexed | 2024-04-11T08:17:30Z |
| format | Article |
| id | doaj.art-efc70d008773479290ce93e583b2f5ef |
| institution | Directory Open Access Journal |
| issn | 2364-5504 |
| language | English |
| last_indexed | 2024-04-11T08:17:30Z |
| publishDate | 2019-09-01 |
| publisher | De Gruyter |
| record_format | Article |
| series | Current Directions in Biomedical Engineering |
| spelling | doaj.art-efc70d008773479290ce93e583b2f5ef2022-12-22T04:35:04ZengDe GruyterCurrent Directions in Biomedical Engineering2364-55042019-09-015129329510.1515/cdbme-2019-0074cdbme-2019-0074Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training PurposesPongratz Christina0Ziegle Jens1Boese Axel2Friebe Michael3Linge Helena4Walles Thorsten5INKA Institute of Medical Technology, Otto-von-Guericke-University (OVGU),Magdeburg, GermanyINKA Institute of Medical Technology, OVGU,Magdeburg, GermanyINKA Institute of Medical Technology, OVGU,Magdeburg, GermanyINKA Institute of Medical Technology, OVGU,Magdeburg, GermanyDepartment of Thoracic Surgery, University Clinic for Cardiac and Thoracic Surgery, Otto-von- Guericke University,Magdeburg, GermanyDepartment of Thoracic Surgery, University Clinic for Cardiac and Thoracic Surgery, Otto-von- Guericke University,Magdeburg, GermanyEx vivo lung perfusion (EVLP) is a preservation method for donor lungs, which keep lungs viable in a physiological environment outside of a body for a short period of time. EVLP is established clinically for lung transplantation. Experimental applications for EVLP are e.g. lung cancer research or medical device development and testing. For preservation, a lung is ventilated artificially in an organ chamber and perfused antegrade through the pulmonary artery. Here we introduce a thermoregulation system for an experimental EVLP system to be used for translational research approaches as well as for training medical staff. To implement physiological culture conditions that are a prerequisite for lung preservation and tissue homeostasis, a thermoregulation is needed to rewarm the explanted lung tissue (storage temperature 4°C). Technically, the EVLP system must be thermally insulated, so loss of caloric is avoided. For monitoring, temperature sensors are integrated within the lung, in the organ chamber and in the afferent perfusate tube, whereby the measured values determine the thermoregulation. Initial tests using thermal packs (cooled to 4-6°C) placed on a heating mat, as a part of the perfusion circuit, showed that the perfusate temperature falls to 34°C, but restores after approximately 60 minutes (36.5°C), whereby the thermal pack is warmed. With this setup longer perfusion times should be obtained rather than without thermoregulation due to normothermic perfusion of the lung.https://doi.org/10.1515/cdbme-2019-0074ex vivo lung perfusionex vivolung perfusiontemperature controltemperature adjustment |
| spellingShingle | Pongratz Christina Ziegle Jens Boese Axel Friebe Michael Linge Helena Walles Thorsten Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes Current Directions in Biomedical Engineering ex vivo lung perfusion ex vivo lung perfusion temperature control temperature adjustment |
| title | Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes |
| title_full | Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes |
| title_fullStr | Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes |
| title_full_unstemmed | Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes |
| title_short | Temperature Controlled and Monitored Ex Vivo Lung Perfusion System for Research and Training Purposes |
| title_sort | temperature controlled and monitored ex vivo lung perfusion system for research and training purposes |
| topic | ex vivo lung perfusion ex vivo lung perfusion temperature control temperature adjustment |
| url | https://doi.org/10.1515/cdbme-2019-0074 |
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