Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern
Rapid emergence of the SARS-CoV-2 variants has dampened the protective efficacy of existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine immunogenicity by presenting multiple copies of desired antigens in a repetitive manner which closely mimics natural infection. We...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Viruses |
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| Online Access: | https://www.mdpi.com/1999-4915/15/2/346 |
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| author | Sara Khaleeq Nayanika Sengupta Sahil Kumar Unnatiben Rajeshbhai Patel Raju S. Rajmani Poorvi Reddy Suman Pandey Randhir Singh Somnath Dutta Rajesh P. Ringe Raghavan Varadarajan |
| author_facet | Sara Khaleeq Nayanika Sengupta Sahil Kumar Unnatiben Rajeshbhai Patel Raju S. Rajmani Poorvi Reddy Suman Pandey Randhir Singh Somnath Dutta Rajesh P. Ringe Raghavan Varadarajan |
| author_sort | Sara Khaleeq |
| collection | DOAJ |
| description | Rapid emergence of the SARS-CoV-2 variants has dampened the protective efficacy of existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine immunogenicity by presenting multiple copies of desired antigens in a repetitive manner which closely mimics natural infection. We have applied nanoparticle display combined with the SpyTag–SpyCatcher system to design encapsulin–mRBD, a nanoparticle vaccine displaying 180 copies of the monomeric SARS-CoV-2 spike receptor-binding domain (RBD). Here we show that encapsulin–mRBD is strongly antigenic and thermotolerant for long durations. After two immunizations, squalene-in-water emulsion (SWE)-adjuvanted encapsulin–mRBD in mice induces potent and comparable neutralizing antibody titers of 10<sup>5</sup> against wild-type (B.1), alpha, beta, and delta variants of concern. Sera also neutralizes the recent Omicron with appreciable neutralization titers, and significant neutralization is observed even after a single immunization. |
| first_indexed | 2024-03-11T08:01:14Z |
| format | Article |
| id | doaj.art-efc76dad569d47eaa3964b1b0a1738c5 |
| institution | Directory Open Access Journal |
| issn | 1999-4915 |
| language | English |
| last_indexed | 2024-03-11T08:01:14Z |
| publishDate | 2023-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj.art-efc76dad569d47eaa3964b1b0a1738c52023-11-16T23:47:46ZengMDPI AGViruses1999-49152023-01-0115234610.3390/v15020346Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of ConcernSara Khaleeq0Nayanika Sengupta1Sahil Kumar2Unnatiben Rajeshbhai Patel3Raju S. Rajmani4Poorvi Reddy5Suman Pandey6Randhir Singh7Somnath Dutta8Rajesh P. Ringe9Raghavan Varadarajan10Molecular Biophysics Unit (MBU), Indian Institute of Science, Bengaluru 560012, IndiaMolecular Biophysics Unit (MBU), Indian Institute of Science, Bengaluru 560012, IndiaVirology Unit, Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Chandigarh 160036, IndiaMynvax Private Limited, 3rd Floor, Brigade MLR Centre, No. 50, Vani Vilas Road, Basavanagudi, Bengaluru 560004, IndiaMolecular Biophysics Unit (MBU), Indian Institute of Science, Bengaluru 560012, IndiaMynvax Private Limited, 3rd Floor, Brigade MLR Centre, No. 50, Vani Vilas Road, Basavanagudi, Bengaluru 560004, IndiaMynvax Private Limited, 3rd Floor, Brigade MLR Centre, No. 50, Vani Vilas Road, Basavanagudi, Bengaluru 560004, IndiaMynvax Private Limited, 3rd Floor, Brigade MLR Centre, No. 50, Vani Vilas Road, Basavanagudi, Bengaluru 560004, IndiaMolecular Biophysics Unit (MBU), Indian Institute of Science, Bengaluru 560012, IndiaVirology Unit, Institute of Microbial Technology, Council of Scientific and Industrial Research (CSIR), Chandigarh 160036, IndiaMolecular Biophysics Unit (MBU), Indian Institute of Science, Bengaluru 560012, IndiaRapid emergence of the SARS-CoV-2 variants has dampened the protective efficacy of existing authorized vaccines. Nanoparticle platforms offer a means to improve vaccine immunogenicity by presenting multiple copies of desired antigens in a repetitive manner which closely mimics natural infection. We have applied nanoparticle display combined with the SpyTag–SpyCatcher system to design encapsulin–mRBD, a nanoparticle vaccine displaying 180 copies of the monomeric SARS-CoV-2 spike receptor-binding domain (RBD). Here we show that encapsulin–mRBD is strongly antigenic and thermotolerant for long durations. After two immunizations, squalene-in-water emulsion (SWE)-adjuvanted encapsulin–mRBD in mice induces potent and comparable neutralizing antibody titers of 10<sup>5</sup> against wild-type (B.1), alpha, beta, and delta variants of concern. Sera also neutralizes the recent Omicron with appreciable neutralization titers, and significant neutralization is observed even after a single immunization.https://www.mdpi.com/1999-4915/15/2/346SpyTagSpyCatcherSWE adjuvantOmicronencapsulinthermotolerant |
| spellingShingle | Sara Khaleeq Nayanika Sengupta Sahil Kumar Unnatiben Rajeshbhai Patel Raju S. Rajmani Poorvi Reddy Suman Pandey Randhir Singh Somnath Dutta Rajesh P. Ringe Raghavan Varadarajan Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern Viruses SpyTag SpyCatcher SWE adjuvant Omicron encapsulin thermotolerant |
| title | Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern |
| title_full | Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern |
| title_fullStr | Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern |
| title_full_unstemmed | Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern |
| title_short | Neutralizing Efficacy of Encapsulin Nanoparticles against SARS-CoV2 Variants of Concern |
| title_sort | neutralizing efficacy of encapsulin nanoparticles against sars cov2 variants of concern |
| topic | SpyTag SpyCatcher SWE adjuvant Omicron encapsulin thermotolerant |
| url | https://www.mdpi.com/1999-4915/15/2/346 |
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