Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells
Abstract Background We have reported that Chinese herbs Astragalus polysaccharide (APS) can inhibit nuclear factor kappaB (NF-κB) activity during the development of diabetic nephropathy in mice. NF-κB plays important roles in genesis, growth, development and metastasis of cancer. NF-κB is also invol...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2017-12-01
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| Series: | Cancer Cell International |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12935-017-0487-6 |
| _version_ | 1831679646407589888 |
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| author | Chao-Yan Wu Yuan Ke Yi-Fei Zeng Ying-Wen Zhang Hai-Jun Yu |
| author_facet | Chao-Yan Wu Yuan Ke Yi-Fei Zeng Ying-Wen Zhang Hai-Jun Yu |
| author_sort | Chao-Yan Wu |
| collection | DOAJ |
| description | Abstract Background We have reported that Chinese herbs Astragalus polysaccharide (APS) can inhibit nuclear factor kappaB (NF-κB) activity during the development of diabetic nephropathy in mice. NF-κB plays important roles in genesis, growth, development and metastasis of cancer. NF-κB is also involved in the development of treatment resistance in tumors. Here we investigated the antitumor activity of APS in human non-small cell lung cells (A549 and NCI-H358) and the related mechanisms of action. Methods The dose–effect and time-effect of antitumor of APS were determined in human lung cancer cell line A549 and NCI-H358. The inhibition effect of APS on the P65 mRNA and protein was detected by reverse transcriptase-PCR (RT-PCR) and Western blot in A549 cells respectively. The inhibition effect of APS on the p50, CyclinD1 and Bcl-xL protein was detected by Western blot in A549 cells respectively. The effect of APS on NF-κB transcription activity was measured with NF-κB luciferase detection. Finally, the nude mice A549 xenograft was introduced to confirm the antitumor activity of APS in vivo. Results Cell viability detection results indicated that APS can inhibit the proliferation of human lung cancer cell line A549 and NCI-H358 in the concentration of 20 and 40 mg/mL. NF-κB activator Phorbol 12-myristate13-acetate (PMA) can attenuate the antitumor activity of APS in both cell lines, but NF-κB inhibitor BAY 11-7082 (Bay) can enhance the effect of APS in both cell lines. In vivo APS can delay the growth of A549 xenograft in BALB/C nude mice. APS can down-regulate the expression of P65 mRNA and protein of A549 cells and decrease the expression of p50, CyclinD1 and Bcl-xL protein. The luciferase detection showed that the APS could reduce the P65 transcription activity in A549 cells. PMA can partially alleviate the inhibition activity of P65 transcription activity of APS in A549 cells, and Bay can enhance the down-regulation of the P65 transcription activity induced by APS in A549 cells. Conclusion APS has a significant antitumor activity in human lung cancer cells A549 and NCI-H358. NF-κB inhibition may mediate the antitumor effect. |
| first_indexed | 2024-12-20T05:24:28Z |
| format | Article |
| id | doaj.art-efc7d8fa2be747bea075386e95dc0d19 |
| institution | Directory Open Access Journal |
| issn | 1475-2867 |
| language | English |
| last_indexed | 2024-12-20T05:24:28Z |
| publishDate | 2017-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Cell International |
| spelling | doaj.art-efc7d8fa2be747bea075386e95dc0d192022-12-21T19:51:56ZengBMCCancer Cell International1475-28672017-12-011711910.1186/s12935-017-0487-6Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cellsChao-Yan Wu0Yuan Ke1Yi-Fei Zeng2Ying-Wen Zhang3Hai-Jun Yu4Department of Integrated Traditional Chinese Medicine and Western Medicine, Zhongnan Hospital of Wuhan UniversityDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan UniversityDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan UniversityDepartment of Integrated Traditional Chinese Medicine and Western Medicine, Zhongnan Hospital of Wuhan UniversityDepartment of Radiation and Medical Oncology, Zhongnan Hospital of Wuhan UniversityAbstract Background We have reported that Chinese herbs Astragalus polysaccharide (APS) can inhibit nuclear factor kappaB (NF-κB) activity during the development of diabetic nephropathy in mice. NF-κB plays important roles in genesis, growth, development and metastasis of cancer. NF-κB is also involved in the development of treatment resistance in tumors. Here we investigated the antitumor activity of APS in human non-small cell lung cells (A549 and NCI-H358) and the related mechanisms of action. Methods The dose–effect and time-effect of antitumor of APS were determined in human lung cancer cell line A549 and NCI-H358. The inhibition effect of APS on the P65 mRNA and protein was detected by reverse transcriptase-PCR (RT-PCR) and Western blot in A549 cells respectively. The inhibition effect of APS on the p50, CyclinD1 and Bcl-xL protein was detected by Western blot in A549 cells respectively. The effect of APS on NF-κB transcription activity was measured with NF-κB luciferase detection. Finally, the nude mice A549 xenograft was introduced to confirm the antitumor activity of APS in vivo. Results Cell viability detection results indicated that APS can inhibit the proliferation of human lung cancer cell line A549 and NCI-H358 in the concentration of 20 and 40 mg/mL. NF-κB activator Phorbol 12-myristate13-acetate (PMA) can attenuate the antitumor activity of APS in both cell lines, but NF-κB inhibitor BAY 11-7082 (Bay) can enhance the effect of APS in both cell lines. In vivo APS can delay the growth of A549 xenograft in BALB/C nude mice. APS can down-regulate the expression of P65 mRNA and protein of A549 cells and decrease the expression of p50, CyclinD1 and Bcl-xL protein. The luciferase detection showed that the APS could reduce the P65 transcription activity in A549 cells. PMA can partially alleviate the inhibition activity of P65 transcription activity of APS in A549 cells, and Bay can enhance the down-regulation of the P65 transcription activity induced by APS in A549 cells. Conclusion APS has a significant antitumor activity in human lung cancer cells A549 and NCI-H358. NF-κB inhibition may mediate the antitumor effect.http://link.springer.com/article/10.1186/s12935-017-0487-6Astragalus polysaccharideAnticancer activityNon-small cell lung cancer cellsNF-κB |
| spellingShingle | Chao-Yan Wu Yuan Ke Yi-Fei Zeng Ying-Wen Zhang Hai-Jun Yu Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells Cancer Cell International Astragalus polysaccharide Anticancer activity Non-small cell lung cancer cells NF-κB |
| title | Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells |
| title_full | Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells |
| title_fullStr | Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells |
| title_full_unstemmed | Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells |
| title_short | Anticancer activity of Astragalus polysaccharide in human non-small cell lung cancer cells |
| title_sort | anticancer activity of astragalus polysaccharide in human non small cell lung cancer cells |
| topic | Astragalus polysaccharide Anticancer activity Non-small cell lung cancer cells NF-κB |
| url | http://link.springer.com/article/10.1186/s12935-017-0487-6 |
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