CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation
CAPPs are putative Primase-Polymerases associated with CRISPR-Cas operons. Here, the authors show CAPPs genetic and physical association with Cas1 and Cas2, their capacity to function as DNA-dependent DNA primases and DNA polymerases, and that Cas1-Cas2 complex adjacent to CAPP has bona fide spacer...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2021-06-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-021-23535-9 |
| _version_ | 1818722969010044928 |
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| author | Katerina Zabrady Matej Zabrady Peter Kolesar Arthur W. H. Li Aidan J. Doherty |
| author_facet | Katerina Zabrady Matej Zabrady Peter Kolesar Arthur W. H. Li Aidan J. Doherty |
| author_sort | Katerina Zabrady |
| collection | DOAJ |
| description | CAPPs are putative Primase-Polymerases associated with CRISPR-Cas operons. Here, the authors show CAPPs genetic and physical association with Cas1 and Cas2, their capacity to function as DNA-dependent DNA primases and DNA polymerases, and that Cas1-Cas2 complex adjacent to CAPP has bona fide spacer integration activity. |
| first_indexed | 2024-12-17T21:03:04Z |
| format | Article |
| id | doaj.art-efc9c689cc494105b57df2aab55a9b23 |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T21:03:04Z |
| publishDate | 2021-06-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-efc9c689cc494105b57df2aab55a9b232022-12-21T21:32:41ZengNature PortfolioNature Communications2041-17232021-06-0112111810.1038/s41467-021-23535-9CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptationKaterina Zabrady0Matej Zabrady1Peter Kolesar2Arthur W. H. Li3Aidan J. Doherty4Genome Damage and Stability Centre, School of Life Sciences, University of SussexGenome Damage and Stability Centre, School of Life Sciences, University of SussexGenome Damage and Stability Centre, School of Life Sciences, University of SussexGenome Damage and Stability Centre, School of Life Sciences, University of SussexGenome Damage and Stability Centre, School of Life Sciences, University of SussexCAPPs are putative Primase-Polymerases associated with CRISPR-Cas operons. Here, the authors show CAPPs genetic and physical association with Cas1 and Cas2, their capacity to function as DNA-dependent DNA primases and DNA polymerases, and that Cas1-Cas2 complex adjacent to CAPP has bona fide spacer integration activity.https://doi.org/10.1038/s41467-021-23535-9 |
| spellingShingle | Katerina Zabrady Matej Zabrady Peter Kolesar Arthur W. H. Li Aidan J. Doherty CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation Nature Communications |
| title | CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation |
| title_full | CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation |
| title_fullStr | CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation |
| title_full_unstemmed | CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation |
| title_short | CRISPR-Associated Primase-Polymerases are implicated in prokaryotic CRISPR-Cas adaptation |
| title_sort | crispr associated primase polymerases are implicated in prokaryotic crispr cas adaptation |
| url | https://doi.org/10.1038/s41467-021-23535-9 |
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