CsBPC2 is essential for cucumber survival under cold stress
Abstract Cold stress affects the growth and development of cucumbers. Whether the BPC2 transcription factor participates in cold tolerance and its regulatory mechanism in plants have not been reported. Here, we used wild-type (WT) cucumber seedlings and two mutant Csbpc2 lines as materials. The unde...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-11-01
|
| Series: | BMC Plant Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12870-023-04577-1 |
| _version_ | 1797577142911893504 |
|---|---|
| author | Di Meng Shuzhen Li Xiaojie Feng Qinghua Di Mengdi Zhou Xianchang Yu Chaoxing He Yan Yan Jun Wang Mintao Sun Yansu Li |
| author_facet | Di Meng Shuzhen Li Xiaojie Feng Qinghua Di Mengdi Zhou Xianchang Yu Chaoxing He Yan Yan Jun Wang Mintao Sun Yansu Li |
| author_sort | Di Meng |
| collection | DOAJ |
| description | Abstract Cold stress affects the growth and development of cucumbers. Whether the BPC2 transcription factor participates in cold tolerance and its regulatory mechanism in plants have not been reported. Here, we used wild-type (WT) cucumber seedlings and two mutant Csbpc2 lines as materials. The underlying mechanisms were studied by determining the phenotype, physiological and biochemical indicators, and transcriptome after cold stress. The results showed that CsBPC2 knockout reduced cucumber cold tolerance by increasing the chilling injury index, relative electrical conductivity and malondialdehyde (MDA) content and decreasing antioxidant enzyme activity. We then conducted RNA sequencing (RNA-seq) to explore transcript-level changes in Csbpc2 mutants. A large number of differentially expressed genes (1032) were identified and found to be unique in Csbpc2 mutants. However, only 489 down-regulated genes related to the synthesis and transport of amino acids and vitamins were found to be enriched through GO analysis. Moreover, both RNA-seq and qPT-PCR techniques revealed that CsBPC2 knockout also decreased the expression of some key cold-responsive genes, such as CsICE1, CsCOR413IM2, CsBZR1 and CsBZR2. These results strongly suggested that CsBPC2 knockout not only affected cold function genes but also decreased the levels of some key metabolites under cold stress. In conclusion, this study reveals for the first time that CsBPC2 is essential for cold tolerance in cucumber and provides a reference for research on the biological function of BPC2 in other plants. |
| first_indexed | 2024-03-10T22:03:50Z |
| format | Article |
| id | doaj.art-efcbe98462374e95aa15cd91fab5f7b4 |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-10T22:03:50Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-efcbe98462374e95aa15cd91fab5f7b42023-11-19T12:52:22ZengBMCBMC Plant Biology1471-22292023-11-0123111410.1186/s12870-023-04577-1CsBPC2 is essential for cucumber survival under cold stressDi Meng0Shuzhen Li1Xiaojie Feng2Qinghua Di3Mengdi Zhou4Xianchang Yu5Chaoxing He6Yan Yan7Jun Wang8Mintao Sun9Yansu Li10State Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesGanzhou Key Laboratory of Greenhouse Vegetable, College of Life Science, Gannan Normal UniversityState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesState Key Laboratory of Vegetable Biobreeding, Institute of Vegetables and Flowers, Chinese Academy of Agricultural SciencesAbstract Cold stress affects the growth and development of cucumbers. Whether the BPC2 transcription factor participates in cold tolerance and its regulatory mechanism in plants have not been reported. Here, we used wild-type (WT) cucumber seedlings and two mutant Csbpc2 lines as materials. The underlying mechanisms were studied by determining the phenotype, physiological and biochemical indicators, and transcriptome after cold stress. The results showed that CsBPC2 knockout reduced cucumber cold tolerance by increasing the chilling injury index, relative electrical conductivity and malondialdehyde (MDA) content and decreasing antioxidant enzyme activity. We then conducted RNA sequencing (RNA-seq) to explore transcript-level changes in Csbpc2 mutants. A large number of differentially expressed genes (1032) were identified and found to be unique in Csbpc2 mutants. However, only 489 down-regulated genes related to the synthesis and transport of amino acids and vitamins were found to be enriched through GO analysis. Moreover, both RNA-seq and qPT-PCR techniques revealed that CsBPC2 knockout also decreased the expression of some key cold-responsive genes, such as CsICE1, CsCOR413IM2, CsBZR1 and CsBZR2. These results strongly suggested that CsBPC2 knockout not only affected cold function genes but also decreased the levels of some key metabolites under cold stress. In conclusion, this study reveals for the first time that CsBPC2 is essential for cold tolerance in cucumber and provides a reference for research on the biological function of BPC2 in other plants.https://doi.org/10.1186/s12870-023-04577-1CucumberCold stressCsBPC2Transcriptome sequence |
| spellingShingle | Di Meng Shuzhen Li Xiaojie Feng Qinghua Di Mengdi Zhou Xianchang Yu Chaoxing He Yan Yan Jun Wang Mintao Sun Yansu Li CsBPC2 is essential for cucumber survival under cold stress BMC Plant Biology Cucumber Cold stress CsBPC2 Transcriptome sequence |
| title | CsBPC2 is essential for cucumber survival under cold stress |
| title_full | CsBPC2 is essential for cucumber survival under cold stress |
| title_fullStr | CsBPC2 is essential for cucumber survival under cold stress |
| title_full_unstemmed | CsBPC2 is essential for cucumber survival under cold stress |
| title_short | CsBPC2 is essential for cucumber survival under cold stress |
| title_sort | csbpc2 is essential for cucumber survival under cold stress |
| topic | Cucumber Cold stress CsBPC2 Transcriptome sequence |
| url | https://doi.org/10.1186/s12870-023-04577-1 |
| work_keys_str_mv | AT dimeng csbpc2isessentialforcucumbersurvivalundercoldstress AT shuzhenli csbpc2isessentialforcucumbersurvivalundercoldstress AT xiaojiefeng csbpc2isessentialforcucumbersurvivalundercoldstress AT qinghuadi csbpc2isessentialforcucumbersurvivalundercoldstress AT mengdizhou csbpc2isessentialforcucumbersurvivalundercoldstress AT xianchangyu csbpc2isessentialforcucumbersurvivalundercoldstress AT chaoxinghe csbpc2isessentialforcucumbersurvivalundercoldstress AT yanyan csbpc2isessentialforcucumbersurvivalundercoldstress AT junwang csbpc2isessentialforcucumbersurvivalundercoldstress AT mintaosun csbpc2isessentialforcucumbersurvivalundercoldstress AT yansuli csbpc2isessentialforcucumbersurvivalundercoldstress |