Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophrenia
Abstract Schizophrenia (SCZ) is a chronic, highly relapsing, severe mental disorder with an unclear etiology. Cytokine-mediated neuroimmune abnormalities have been repeatedly revealed. IL-1β was reported to play a vital role in expanding the inflammatory response. However, the underlying molecular m...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-11-01
|
| Series: | BMC Psychiatry |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12888-023-05182-0 |
| _version_ | 1797630066268569600 |
|---|---|
| author | Xin-ling Zhao Yi-lin Liu Qing Long Yun-qiao Zhang Xu You Ze-yi Guo Xiang Cao Lei Yu Fu-yi Qin Zhao-wei Teng Yong Zeng |
| author_facet | Xin-ling Zhao Yi-lin Liu Qing Long Yun-qiao Zhang Xu You Ze-yi Guo Xiang Cao Lei Yu Fu-yi Qin Zhao-wei Teng Yong Zeng |
| author_sort | Xin-ling Zhao |
| collection | DOAJ |
| description | Abstract Schizophrenia (SCZ) is a chronic, highly relapsing, severe mental disorder with an unclear etiology. Cytokine-mediated neuroimmune abnormalities have been repeatedly revealed. IL-1β was reported to play a vital role in expanding the inflammatory response. However, the underlying molecular mechanism is poorly understood. In this study, we found that miR-3653-3p with the NLRP3 binding site in Targetscan was differentially expressed in miRNA high-throughput sequencing in schizophrenia (SCZ), and indeed, its downregulation in SCZ peripheral blood was also verified by RT-qPCR (P-value = 0.015). Furthermore, we found that the mRNAs of caspase 1 and IL-1β are elevated in people who suffer from SCZ (P = 0.044 and P = 0.001, respectively). Moreover, the interaction of NLRP3, Caspase1, and IL-1β was found in the peripheral blood of patients with SCZ. The expression level of miR-3653-3p was negatively correlated with NLRP3 and IL-1β mRNA contents (r = 0.487, P = 0.04 and r = 0.508, P = 0.037, respectively). NLRP3 mRNA was positively correlated with caspase1 mRNA. Meanwhile, the expression of miR-3653-3p was also negatively correlated with negative symptom subscores of PANSS (r = 0.450, P = 0.046). IL-1β mRNA is positively correlated with the total scores of PANSS (r = 0.690, P = 0.002) and the sub-scores of general psychopathology of PANSS (r = 0.583, P = 0.014). Additionally, a significant positive relationship exists between IL-1β and the total duration (r = 0.638, P = 0.006). We found that the combination of miR-3653-3p, caspase 1, and IL-1β have better diagnostic values. The results indicate that miR-3653-3p, caspase 1, and IL-1β can potentially be biomarkers of SCZ, identifying negative symptoms or a chronic course. A further understanding of the involvement of IL-1β in SCZ may be a crucial molecular effector for the chronic course to intervene. |
| first_indexed | 2024-03-11T11:02:46Z |
| format | Article |
| id | doaj.art-efcdeae530a9426a9be6bf4f561443a9 |
| institution | Directory Open Access Journal |
| issn | 1471-244X |
| language | English |
| last_indexed | 2024-03-11T11:02:46Z |
| publishDate | 2023-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Psychiatry |
| spelling | doaj.art-efcdeae530a9426a9be6bf4f561443a92023-11-12T12:26:00ZengBMCBMC Psychiatry1471-244X2023-11-0123111010.1186/s12888-023-05182-0Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophreniaXin-ling Zhao0Yi-lin Liu1Qing Long2Yun-qiao Zhang3Xu You4Ze-yi Guo5Xiang Cao6Lei Yu7Fu-yi Qin8Zhao-wei Teng9Yong Zeng10Department of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityDepartment of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityDepartment of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityPsychiatric Ward, Honghe Second People’s HospitalPsychiatric Ward, Honghe Second People’s HospitalDepartment of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityDepartment of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityDepartment of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityDepartment of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityCentral Laboratory of the Second Affiliated Hospital of Kunming Medical UniversityDepartment of Psychiatry, The Second Affiliated Hospital of Kunming Medical UniversityAbstract Schizophrenia (SCZ) is a chronic, highly relapsing, severe mental disorder with an unclear etiology. Cytokine-mediated neuroimmune abnormalities have been repeatedly revealed. IL-1β was reported to play a vital role in expanding the inflammatory response. However, the underlying molecular mechanism is poorly understood. In this study, we found that miR-3653-3p with the NLRP3 binding site in Targetscan was differentially expressed in miRNA high-throughput sequencing in schizophrenia (SCZ), and indeed, its downregulation in SCZ peripheral blood was also verified by RT-qPCR (P-value = 0.015). Furthermore, we found that the mRNAs of caspase 1 and IL-1β are elevated in people who suffer from SCZ (P = 0.044 and P = 0.001, respectively). Moreover, the interaction of NLRP3, Caspase1, and IL-1β was found in the peripheral blood of patients with SCZ. The expression level of miR-3653-3p was negatively correlated with NLRP3 and IL-1β mRNA contents (r = 0.487, P = 0.04 and r = 0.508, P = 0.037, respectively). NLRP3 mRNA was positively correlated with caspase1 mRNA. Meanwhile, the expression of miR-3653-3p was also negatively correlated with negative symptom subscores of PANSS (r = 0.450, P = 0.046). IL-1β mRNA is positively correlated with the total scores of PANSS (r = 0.690, P = 0.002) and the sub-scores of general psychopathology of PANSS (r = 0.583, P = 0.014). Additionally, a significant positive relationship exists between IL-1β and the total duration (r = 0.638, P = 0.006). We found that the combination of miR-3653-3p, caspase 1, and IL-1β have better diagnostic values. The results indicate that miR-3653-3p, caspase 1, and IL-1β can potentially be biomarkers of SCZ, identifying negative symptoms or a chronic course. A further understanding of the involvement of IL-1β in SCZ may be a crucial molecular effector for the chronic course to intervene.https://doi.org/10.1186/s12888-023-05182-0SchizophreniaIL-1βNLRP3miR-3653-3pCaspase 1qPCR |
| spellingShingle | Xin-ling Zhao Yi-lin Liu Qing Long Yun-qiao Zhang Xu You Ze-yi Guo Xiang Cao Lei Yu Fu-yi Qin Zhao-wei Teng Yong Zeng Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophrenia BMC Psychiatry Schizophrenia IL-1β NLRP3 miR-3653-3p Caspase 1 qPCR |
| title | Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophrenia |
| title_full | Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophrenia |
| title_fullStr | Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophrenia |
| title_full_unstemmed | Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophrenia |
| title_short | Abnormal expression of miR-3653-3p, caspase 1, IL-1β in peripheral blood of schizophrenia |
| title_sort | abnormal expression of mir 3653 3p caspase 1 il 1β in peripheral blood of schizophrenia |
| topic | Schizophrenia IL-1β NLRP3 miR-3653-3p Caspase 1 qPCR |
| url | https://doi.org/10.1186/s12888-023-05182-0 |
| work_keys_str_mv | AT xinlingzhao abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT yilinliu abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT qinglong abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT yunqiaozhang abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT xuyou abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT zeyiguo abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT xiangcao abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT leiyu abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT fuyiqin abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT zhaoweiteng abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia AT yongzeng abnormalexpressionofmir36533pcaspase1il1binperipheralbloodofschizophrenia |