The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance
Inefficiency of medical therapies used in order to cure patients with bacterial infections requires not only to actively look for new therapeutic strategies but also to carefully select antibiotics based on variety of parameters, including microbiological. Minimal inhibitory concentration (MIC) defi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/10/2/165 |
| _version_ | 1797415373030555648 |
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| author | Beata Kowalska-Krochmal Ruth Dudek-Wicher |
| author_facet | Beata Kowalska-Krochmal Ruth Dudek-Wicher |
| author_sort | Beata Kowalska-Krochmal |
| collection | DOAJ |
| description | Inefficiency of medical therapies used in order to cure patients with bacterial infections requires not only to actively look for new therapeutic strategies but also to carefully select antibiotics based on variety of parameters, including microbiological. Minimal inhibitory concentration (MIC) defines in vitro levels of susceptibility or resistance of specific bacterial strains to applied antibiotic. Reliable assessment of MIC has a significant impact on the choice of a therapeutic strategy, which affects efficiency of an infection therapy. In order to obtain credible MIC, many elements must be considered, such as proper method choice, adherence to labeling rules, and competent interpretation of the results. In this paper, two methods have been discussed: dilution and gradient used for MIC estimation. Factors which affect MIC results along with the interpretation guidelines have been described. Furthermore, opportunities to utilize MIC in clinical practice, with pharmacokinetic /pharmacodynamic parameters taken into consideration, have been investigated. Due to problems related to PK determination in individual patients, statistical estimation of the possibility of achievement of the PK/PD index, based on the Monte Carlo, was discussed. In order to provide comprehensive insights, the possible limitations of MIC, which scientists are aware of, have been outlined. |
| first_indexed | 2024-03-09T05:46:39Z |
| format | Article |
| id | doaj.art-efce00235a094f04b0ddc8b179fbd79d |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T05:46:39Z |
| publishDate | 2021-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-efce00235a094f04b0ddc8b179fbd79d2023-12-03T12:20:41ZengMDPI AGPathogens2076-08172021-02-0110216510.3390/pathogens10020165The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical RelevanceBeata Kowalska-Krochmal0Ruth Dudek-Wicher1Department of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Medical University of Silesian Piasts in Wroclaw, Borowska 211, 50-556 Wroclaw, PolandDepartment of Pharmaceutical Microbiology and Parasitology, Faculty of Pharmacy, Medical University of Silesian Piasts in Wroclaw, Borowska 211, 50-556 Wroclaw, PolandInefficiency of medical therapies used in order to cure patients with bacterial infections requires not only to actively look for new therapeutic strategies but also to carefully select antibiotics based on variety of parameters, including microbiological. Minimal inhibitory concentration (MIC) defines in vitro levels of susceptibility or resistance of specific bacterial strains to applied antibiotic. Reliable assessment of MIC has a significant impact on the choice of a therapeutic strategy, which affects efficiency of an infection therapy. In order to obtain credible MIC, many elements must be considered, such as proper method choice, adherence to labeling rules, and competent interpretation of the results. In this paper, two methods have been discussed: dilution and gradient used for MIC estimation. Factors which affect MIC results along with the interpretation guidelines have been described. Furthermore, opportunities to utilize MIC in clinical practice, with pharmacokinetic /pharmacodynamic parameters taken into consideration, have been investigated. Due to problems related to PK determination in individual patients, statistical estimation of the possibility of achievement of the PK/PD index, based on the Monte Carlo, was discussed. In order to provide comprehensive insights, the possible limitations of MIC, which scientists are aware of, have been outlined.https://www.mdpi.com/2076-0817/10/2/165minimal inhibitory concentrationantibioticsdilution and gradient methods, interpretation of resultsthe importance of MIC value |
| spellingShingle | Beata Kowalska-Krochmal Ruth Dudek-Wicher The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance Pathogens minimal inhibitory concentration antibiotics dilution and gradient methods, interpretation of results the importance of MIC value |
| title | The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance |
| title_full | The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance |
| title_fullStr | The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance |
| title_full_unstemmed | The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance |
| title_short | The Minimum Inhibitory Concentration of Antibiotics: Methods, Interpretation, Clinical Relevance |
| title_sort | minimum inhibitory concentration of antibiotics methods interpretation clinical relevance |
| topic | minimal inhibitory concentration antibiotics dilution and gradient methods, interpretation of results the importance of MIC value |
| url | https://www.mdpi.com/2076-0817/10/2/165 |
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