Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure
Ascent to high altitude feels uncomfortable in part because of a decreased partial pressure of oxygen due to the decrease in barometric pressure. The molecular mechanisms causing injury in liver tissue after exposure to a hypoxic environment are widely unknown. The liver must physiologically and met...
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| Format: | Article |
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PeerJ Inc.
2019-04-01
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| Online Access: | https://peerj.com/articles/6499.pdf |
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| author | Zhenguo Xu Zhilong Jia Jinlong Shi Zeyu Zhang Xiaojian Gao Qian Jia Bohan Liu Jixuan Liu Chunlei Liu Xiaojing Zhao Kunlun He |
| author_facet | Zhenguo Xu Zhilong Jia Jinlong Shi Zeyu Zhang Xiaojian Gao Qian Jia Bohan Liu Jixuan Liu Chunlei Liu Xiaojing Zhao Kunlun He |
| author_sort | Zhenguo Xu |
| collection | DOAJ |
| description | Ascent to high altitude feels uncomfortable in part because of a decreased partial pressure of oxygen due to the decrease in barometric pressure. The molecular mechanisms causing injury in liver tissue after exposure to a hypoxic environment are widely unknown. The liver must physiologically and metabolically change to improve tolerance to altitude-induced hypoxia. Since the liver is the largest metabolic organ and regulates many physiological and metabolic processes, it plays an important part in high altitude adaptation. The cellular response to hypoxia results in changes in the gene expression profile. The present study explores these changes in a rat model. To comprehensively investigate the gene expression and physiological changes under hypobaric hypoxia, we used genome-wide transcription profiling. Little is known about the genome-wide transcriptional response to acute and chronic hypobaric hypoxia in the livers of rats. In this study, we carried out RNA-Sequencing (RNA-Seq) of liver tissue from rats in three groups, normal control rats (L), rats exposed to acute hypobaric hypoxia for 2 weeks (W2L) and rats chronically exposed to hypobaric hypoxia for 4 weeks (W4L), to explore the transcriptional profile of acute and chronic mountain sickness in a mammal under a controlled time-course. We identified 497 differentially expressed genes between the three groups. A principal component analysis revealed large differences between the acute and chronic hypobaric hypoxia groups compared with the control group. Several immune-related and metabolic pathways, such as cytokine-cytokine receptor interaction and galactose metabolism, were highly enriched in the KEGG pathway analysis. Similar results were found in the Gene Ontology analysis. Cogena analysis showed that the immune-related pathways were mainly upregulated and enriched in the acute hypobaric hypoxia group. |
| first_indexed | 2024-03-09T06:31:35Z |
| format | Article |
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| institution | Directory Open Access Journal |
| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:31:35Z |
| publishDate | 2019-04-01 |
| publisher | PeerJ Inc. |
| record_format | Article |
| series | PeerJ |
| spelling | doaj.art-efcea516ded54424bd90a3390d67b3242023-12-03T11:05:12ZengPeerJ Inc.PeerJ2167-83592019-04-017e649910.7717/peerj.6499Transcriptional profiling in the livers of rats after hypobaric hypoxia exposureZhenguo Xu0Zhilong Jia1Jinlong Shi2Zeyu Zhang3Xiaojian Gao4Qian Jia5Bohan Liu6Jixuan Liu7Chunlei Liu8Xiaojing Zhao9Kunlun He10Laboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaBeijing Key Laboratory of Chronic Heart Failure Precision Medicine, Chinese PLA General Hospital, Beijing, ChinaBeijing Key Laboratory of Chronic Heart Failure Precision Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaLaboratory of Translational Medicine, Chinese PLA General Hospital, Beijing, ChinaAscent to high altitude feels uncomfortable in part because of a decreased partial pressure of oxygen due to the decrease in barometric pressure. The molecular mechanisms causing injury in liver tissue after exposure to a hypoxic environment are widely unknown. The liver must physiologically and metabolically change to improve tolerance to altitude-induced hypoxia. Since the liver is the largest metabolic organ and regulates many physiological and metabolic processes, it plays an important part in high altitude adaptation. The cellular response to hypoxia results in changes in the gene expression profile. The present study explores these changes in a rat model. To comprehensively investigate the gene expression and physiological changes under hypobaric hypoxia, we used genome-wide transcription profiling. Little is known about the genome-wide transcriptional response to acute and chronic hypobaric hypoxia in the livers of rats. In this study, we carried out RNA-Sequencing (RNA-Seq) of liver tissue from rats in three groups, normal control rats (L), rats exposed to acute hypobaric hypoxia for 2 weeks (W2L) and rats chronically exposed to hypobaric hypoxia for 4 weeks (W4L), to explore the transcriptional profile of acute and chronic mountain sickness in a mammal under a controlled time-course. We identified 497 differentially expressed genes between the three groups. A principal component analysis revealed large differences between the acute and chronic hypobaric hypoxia groups compared with the control group. Several immune-related and metabolic pathways, such as cytokine-cytokine receptor interaction and galactose metabolism, were highly enriched in the KEGG pathway analysis. Similar results were found in the Gene Ontology analysis. Cogena analysis showed that the immune-related pathways were mainly upregulated and enriched in the acute hypobaric hypoxia group.https://peerj.com/articles/6499.pdfHypobaric hypoxiaRat modelMountain sicknessTranscriptional analysis |
| spellingShingle | Zhenguo Xu Zhilong Jia Jinlong Shi Zeyu Zhang Xiaojian Gao Qian Jia Bohan Liu Jixuan Liu Chunlei Liu Xiaojing Zhao Kunlun He Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure PeerJ Hypobaric hypoxia Rat model Mountain sickness Transcriptional analysis |
| title | Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure |
| title_full | Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure |
| title_fullStr | Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure |
| title_full_unstemmed | Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure |
| title_short | Transcriptional profiling in the livers of rats after hypobaric hypoxia exposure |
| title_sort | transcriptional profiling in the livers of rats after hypobaric hypoxia exposure |
| topic | Hypobaric hypoxia Rat model Mountain sickness Transcriptional analysis |
| url | https://peerj.com/articles/6499.pdf |
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