Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study.
Compared to expert malaria microscopy, malaria biomarkers such as Plasmodium falciparum histidine rich protein-2 (PfHRP-2), and PCR provide superior analytical sensitivity and specificity for quantifying malaria parasites infections. This study reports on parasite prevalence, sick visits parasite de...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2013-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3598859?pdf=render |
| _version_ | 1818935709078126592 |
|---|---|
| author | Ismail Mahat Bashir Nekoye Otsyula George Awinda Michele Spring Petra Schneider John Njenga Waitumbi |
| author_facet | Ismail Mahat Bashir Nekoye Otsyula George Awinda Michele Spring Petra Schneider John Njenga Waitumbi |
| author_sort | Ismail Mahat Bashir |
| collection | DOAJ |
| description | Compared to expert malaria microscopy, malaria biomarkers such as Plasmodium falciparum histidine rich protein-2 (PfHRP-2), and PCR provide superior analytical sensitivity and specificity for quantifying malaria parasites infections. This study reports on parasite prevalence, sick visits parasite density and species composition by different diagnostic methods during a phase-I malaria vaccine trial.Blood samples for microscopy, PfHRP-2 and Plasmodium lactate dehydrogenase (pLDH) ELISAs and real time quantitative PCR (qPCR) were collected during scheduled (n = 298) or sick visits (n = 38) from 30 adults participating in a 112-day vaccine trial. The four methods were used to assess parasite prevalence, as well as parasite density over a 42-day period for patients with clinical episodes.During scheduled visits, qPCR (39.9%, N = 119) and PfHRP-2 ELISA (36.9%, N = 110) detected higher parasite prevalence than pLDH ELISA (16.8%, N = 50) and all methods were more sensitive than microscopy (13.4%, N = 40). All microscopically detected infections contained P. falciparum, as mono-infections (95%) or with P. malariae (5%). By qPCR, 102/119 infections were speciated. P. falciparum predominated either as monoinfections (71.6%), with P. malariae (8.8%), P. ovale (4.9%) or both (3.9%). P. malariae (6.9%) and P. ovale (1.0%) also occurred as co-infections (2.9%). As expected, higher prevalences were detected during sick visits, with prevalences of 65.8% (qPCR), 60.5% (PfHRP-2 ELISA), 21.1% (pLDH ELISA) and 31.6% (microscopy). PfHRP-2 showed biomass build-up that climaxed (1813±3410 ng/mL SD) at clinical episodes.PfHRP-2 ELISA and qPCR may be needed for accurately quantifying the malaria parasite burden. In addition, qPCR improves parasite speciation, whilst PfHRP-2 ELISA is a potential predictor for clinical disease caused by P. falciparum.ClinicalTrials.gov NCT00666380. |
| first_indexed | 2024-12-20T05:24:28Z |
| format | Article |
| id | doaj.art-efcf76ded6b04dd099e4a1fb3390d1fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T05:24:28Z |
| publishDate | 2013-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-efcf76ded6b04dd099e4a1fb3390d1fc2022-12-21T19:51:56ZengPublic Library of Science (PLoS)PLoS ONE1932-62032013-01-0183e5682810.1371/journal.pone.0056828Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study.Ismail Mahat BashirNekoye OtsyulaGeorge AwindaMichele SpringPetra SchneiderJohn Njenga WaitumbiCompared to expert malaria microscopy, malaria biomarkers such as Plasmodium falciparum histidine rich protein-2 (PfHRP-2), and PCR provide superior analytical sensitivity and specificity for quantifying malaria parasites infections. This study reports on parasite prevalence, sick visits parasite density and species composition by different diagnostic methods during a phase-I malaria vaccine trial.Blood samples for microscopy, PfHRP-2 and Plasmodium lactate dehydrogenase (pLDH) ELISAs and real time quantitative PCR (qPCR) were collected during scheduled (n = 298) or sick visits (n = 38) from 30 adults participating in a 112-day vaccine trial. The four methods were used to assess parasite prevalence, as well as parasite density over a 42-day period for patients with clinical episodes.During scheduled visits, qPCR (39.9%, N = 119) and PfHRP-2 ELISA (36.9%, N = 110) detected higher parasite prevalence than pLDH ELISA (16.8%, N = 50) and all methods were more sensitive than microscopy (13.4%, N = 40). All microscopically detected infections contained P. falciparum, as mono-infections (95%) or with P. malariae (5%). By qPCR, 102/119 infections were speciated. P. falciparum predominated either as monoinfections (71.6%), with P. malariae (8.8%), P. ovale (4.9%) or both (3.9%). P. malariae (6.9%) and P. ovale (1.0%) also occurred as co-infections (2.9%). As expected, higher prevalences were detected during sick visits, with prevalences of 65.8% (qPCR), 60.5% (PfHRP-2 ELISA), 21.1% (pLDH ELISA) and 31.6% (microscopy). PfHRP-2 showed biomass build-up that climaxed (1813±3410 ng/mL SD) at clinical episodes.PfHRP-2 ELISA and qPCR may be needed for accurately quantifying the malaria parasite burden. In addition, qPCR improves parasite speciation, whilst PfHRP-2 ELISA is a potential predictor for clinical disease caused by P. falciparum.ClinicalTrials.gov NCT00666380.http://europepmc.org/articles/PMC3598859?pdf=render |
| spellingShingle | Ismail Mahat Bashir Nekoye Otsyula George Awinda Michele Spring Petra Schneider John Njenga Waitumbi Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study. PLoS ONE |
| title | Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study. |
| title_full | Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study. |
| title_fullStr | Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study. |
| title_full_unstemmed | Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study. |
| title_short | Comparison of PfHRP-2/pLDH ELISA, qPCR and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study. |
| title_sort | comparison of pfhrp 2 pldh elisa qpcr and microscopy for the detection of plasmodium events and prediction of sick visits during a malaria vaccine study |
| url | http://europepmc.org/articles/PMC3598859?pdf=render |
| work_keys_str_mv | AT ismailmahatbashir comparisonofpfhrp2pldhelisaqpcrandmicroscopyforthedetectionofplasmodiumeventsandpredictionofsickvisitsduringamalariavaccinestudy AT nekoyeotsyula comparisonofpfhrp2pldhelisaqpcrandmicroscopyforthedetectionofplasmodiumeventsandpredictionofsickvisitsduringamalariavaccinestudy AT georgeawinda comparisonofpfhrp2pldhelisaqpcrandmicroscopyforthedetectionofplasmodiumeventsandpredictionofsickvisitsduringamalariavaccinestudy AT michelespring comparisonofpfhrp2pldhelisaqpcrandmicroscopyforthedetectionofplasmodiumeventsandpredictionofsickvisitsduringamalariavaccinestudy AT petraschneider comparisonofpfhrp2pldhelisaqpcrandmicroscopyforthedetectionofplasmodiumeventsandpredictionofsickvisitsduringamalariavaccinestudy AT johnnjengawaitumbi comparisonofpfhrp2pldhelisaqpcrandmicroscopyforthedetectionofplasmodiumeventsandpredictionofsickvisitsduringamalariavaccinestudy |