Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma Phenotype
Storage mites (SM) may induce allergic respiratory symptoms in sensitized individuals, in both rural and urban settings. The relationship among specific IgE reactions to determined groups of SM allergens in the coincident asthma pheno-endotypes has not yet been investigated. We aimed to study a Prec...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-04-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/23/8/4297 |
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| author | Ruperto González-Pérez Paloma Poza-Guedes Fernando Pineda Miriam Castillo Inmaculada Sánchez-Machín |
| author_facet | Ruperto González-Pérez Paloma Poza-Guedes Fernando Pineda Miriam Castillo Inmaculada Sánchez-Machín |
| author_sort | Ruperto González-Pérez |
| collection | DOAJ |
| description | Storage mites (SM) may induce allergic respiratory symptoms in sensitized individuals, in both rural and urban settings. The relationship among specific IgE reactions to determined groups of SM allergens in the coincident asthma pheno-endotypes has not yet been investigated. We aimed to study a Precision Allergy Molecular Diagnosis (PAMD@) model to depict the SM molecular profile in individuals presenting with Type-2 inflammation, in two different (moderate and severe) asthma phenotypes. A customized PAMD@ panel, including SM allergens and their concurrent protein allergenic characterization was investigated. Mite group 2 allergens were most frequently recognized, including Lep d 2 (83.45%), followed by Gly d 2 (69.17%) and Tyr p 2 (47,37%), in 133/164 asthmatic subjects. Blo t 5 and Blo t 21 exhibited significant higher titres in both asthma groups. Although relevant mite group 2 allergens cross-reactivity is suggested, individualized sensitization patterns were relevantly identified. The present PAMD@ panel confirmed the dominance of mite group 2 allergens in moderate-to-severe T2 asthmatics. A broadly heterogeneous molecular repertoire of SM allergens was found in all subjects, regardless of their asthma severity. <i>Blomia tropicalis</i> deserves special attention in certain territories, as diagnostic and/or therapeutic approaches merely based on <i>Pyroglyphidae</i> mites may be insufficient. |
| first_indexed | 2024-03-09T10:34:39Z |
| format | Article |
| id | doaj.art-efd155c228244bc5963dd3724f5fe194 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-09T10:34:39Z |
| publishDate | 2022-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-efd155c228244bc5963dd3724f5fe1942023-12-01T21:03:51ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-04-01238429710.3390/ijms23084297Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma PhenotypeRuperto González-Pérez0Paloma Poza-Guedes1Fernando Pineda2Miriam Castillo3Inmaculada Sánchez-Machín4Allergy Department, Hospital Universitario de Canarias, 38320 Tenerife, SpainAllergy Department, Hospital Universitario de Canarias, 38320 Tenerife, SpainDiater Laboratories, 28919 Madrid, SpainDiater Laboratories, 28919 Madrid, SpainAllergy Department, Hospital Universitario de Canarias, 38320 Tenerife, SpainStorage mites (SM) may induce allergic respiratory symptoms in sensitized individuals, in both rural and urban settings. The relationship among specific IgE reactions to determined groups of SM allergens in the coincident asthma pheno-endotypes has not yet been investigated. We aimed to study a Precision Allergy Molecular Diagnosis (PAMD@) model to depict the SM molecular profile in individuals presenting with Type-2 inflammation, in two different (moderate and severe) asthma phenotypes. A customized PAMD@ panel, including SM allergens and their concurrent protein allergenic characterization was investigated. Mite group 2 allergens were most frequently recognized, including Lep d 2 (83.45%), followed by Gly d 2 (69.17%) and Tyr p 2 (47,37%), in 133/164 asthmatic subjects. Blo t 5 and Blo t 21 exhibited significant higher titres in both asthma groups. Although relevant mite group 2 allergens cross-reactivity is suggested, individualized sensitization patterns were relevantly identified. The present PAMD@ panel confirmed the dominance of mite group 2 allergens in moderate-to-severe T2 asthmatics. A broadly heterogeneous molecular repertoire of SM allergens was found in all subjects, regardless of their asthma severity. <i>Blomia tropicalis</i> deserves special attention in certain territories, as diagnostic and/or therapeutic approaches merely based on <i>Pyroglyphidae</i> mites may be insufficient.https://www.mdpi.com/1422-0067/23/8/4297airborne allergensallergen exposureasthmastorage mitesT2 inflammation |
| spellingShingle | Ruperto González-Pérez Paloma Poza-Guedes Fernando Pineda Miriam Castillo Inmaculada Sánchez-Machín Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma Phenotype International Journal of Molecular Sciences airborne allergens allergen exposure asthma storage mites T2 inflammation |
| title | Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma Phenotype |
| title_full | Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma Phenotype |
| title_fullStr | Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma Phenotype |
| title_full_unstemmed | Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma Phenotype |
| title_short | Storage Mite Precision Allergy Molecular Diagnosis in the Moderate-to-Severe T2-High Asthma Phenotype |
| title_sort | storage mite precision allergy molecular diagnosis in the moderate to severe t2 high asthma phenotype |
| topic | airborne allergens allergen exposure asthma storage mites T2 inflammation |
| url | https://www.mdpi.com/1422-0067/23/8/4297 |
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