Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission
(1) Background: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) excretion in stools is well documented by RT-PCR, but evidences that stools contain infectious particles are scarce. (2) Methods: After observing a Corona Virus 2019 Disease (COVID-19) epidemic cluster associated with a rup...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Journal of Clinical Medicine |
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| Online Access: | https://www.mdpi.com/2077-0383/10/12/2696 |
| _version_ | 1797529670141345792 |
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| author | Julie Dergham Jeremy Delerce Marielle Bedotto Bernard La Scola Valérie Moal |
| author_facet | Julie Dergham Jeremy Delerce Marielle Bedotto Bernard La Scola Valérie Moal |
| author_sort | Julie Dergham |
| collection | DOAJ |
| description | (1) Background: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) excretion in stools is well documented by RT-PCR, but evidences that stools contain infectious particles are scarce. (2) Methods: After observing a Corona Virus 2019 Disease (COVID-19) epidemic cluster associated with a ruptured sewage pipe, we search for such a viable SARS-CoV-2 particle in stool by inoculating 106 samples from 46 patients. (3) Results: We successfully obtained two isolates from a unique patient with kidney transplantation under immunosuppressive therapy who was admitted for severe diarrhea. (4) Conclusions: This report emphasizes that SARS-CoV-2 is an enteric virus, and infectious virus particles can be isolated from the stool of immune-compromised patients like, in our case, kidney transplant recipient. Immune-compromised patients are likely to have massive multiplication of the virus in the gastrointestinal tract and this report suggests possible fecal transmission of SARS-CoV-2. |
| first_indexed | 2024-03-10T10:18:04Z |
| format | Article |
| id | doaj.art-efd18388ec0b464f94d009c8eab153b4 |
| institution | Directory Open Access Journal |
| issn | 2077-0383 |
| language | English |
| last_indexed | 2024-03-10T10:18:04Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Clinical Medicine |
| spelling | doaj.art-efd18388ec0b464f94d009c8eab153b42023-11-22T00:44:25ZengMDPI AGJournal of Clinical Medicine2077-03832021-06-011012269610.3390/jcm10122696Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of TransmissionJulie Dergham0Jeremy Delerce1Marielle Bedotto2Bernard La Scola3Valérie Moal4IHU-Méditerranée Infection, 13005 Marseille, FranceIHU-Méditerranée Infection, 13005 Marseille, FranceIHU-Méditerranée Infection, 13005 Marseille, FranceIHU-Méditerranée Infection, 13005 Marseille, FranceInstitut de Recherche pour le Développement (IRD), Assistance Publique Hôpitaux de Marseille, Microbes Evolution Phylogeny and Infections (MEPHI), Aix Marseille Université, 13005 Marseille, France(1) Background: Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) excretion in stools is well documented by RT-PCR, but evidences that stools contain infectious particles are scarce. (2) Methods: After observing a Corona Virus 2019 Disease (COVID-19) epidemic cluster associated with a ruptured sewage pipe, we search for such a viable SARS-CoV-2 particle in stool by inoculating 106 samples from 46 patients. (3) Results: We successfully obtained two isolates from a unique patient with kidney transplantation under immunosuppressive therapy who was admitted for severe diarrhea. (4) Conclusions: This report emphasizes that SARS-CoV-2 is an enteric virus, and infectious virus particles can be isolated from the stool of immune-compromised patients like, in our case, kidney transplant recipient. Immune-compromised patients are likely to have massive multiplication of the virus in the gastrointestinal tract and this report suggests possible fecal transmission of SARS-CoV-2.https://www.mdpi.com/2077-0383/10/12/2696SARS-CoV-2stoolscell cultureCovid-19organ transplantation |
| spellingShingle | Julie Dergham Jeremy Delerce Marielle Bedotto Bernard La Scola Valérie Moal Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission Journal of Clinical Medicine SARS-CoV-2 stools cell culture Covid-19 organ transplantation |
| title | Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission |
| title_full | Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission |
| title_fullStr | Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission |
| title_full_unstemmed | Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission |
| title_short | Isolation of Viable SARS-CoV-2 Virus from Feces of an Immunocompromised Patient Suggesting a Possible Fecal Mode of Transmission |
| title_sort | isolation of viable sars cov 2 virus from feces of an immunocompromised patient suggesting a possible fecal mode of transmission |
| topic | SARS-CoV-2 stools cell culture Covid-19 organ transplantation |
| url | https://www.mdpi.com/2077-0383/10/12/2696 |
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