Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicus
Trained immunity is driven by metabolism and epigenetics in innate immune cells in mammals. The phenomenon of trained immunity has been identified in invertebrates, including shrimp, but the underlying mechanisms remain unclear. To elucidate mechanisms of trained immunity in shrimp, the metabolomic...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2022-05-01
|
| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2022.885782/full |
| _version_ | 1818551355839610880 |
|---|---|
| author | Shaoqing Zang Li-Xia Lv Chen-Fei Liu Peng Zhang Cang Li Jin-Xing Wang Jin-Xing Wang |
| author_facet | Shaoqing Zang Li-Xia Lv Chen-Fei Liu Peng Zhang Cang Li Jin-Xing Wang Jin-Xing Wang |
| author_sort | Shaoqing Zang |
| collection | DOAJ |
| description | Trained immunity is driven by metabolism and epigenetics in innate immune cells in mammals. The phenomenon of trained immunity has been identified in invertebrates, including shrimp, but the underlying mechanisms remain unclear. To elucidate mechanisms of trained immunity in shrimp, the metabolomic changes in hemolymph of Marsupenaeus japonicus trained by the UV-inactivated white spot syndrome virus (UV-WSSV) were analyzed using tandem gas chromatography–mass/mass spectrometry. The metabolomic profiles of shrimp trained with UV-WSSV followed WSSV infection showed significant differences comparison with the control groups, PBS injection followed WSSV infection. 16 differential metabolites in total of 154 metabolites were identified, including D-fructose-6-phosphate, D-glucose-6-phosphate, and D-fructose-6-phosphate, and metabolic pathways, glycolysis, pentose phosphate pathway, and AMPK signaling pathway were enriched in the UV-WSSV trained groups. Further study found that histone monomethylation and trimethylation at H3K4 (H3K4me1 and H3K4me3) were involved in the trained immunity. Our data suggest that the UV-WSSV induced trained immunity leads to metabolism reprogramming in the shrimp and provide insights for WSSV control in shrimp aquaculture. |
| first_indexed | 2024-12-12T08:58:48Z |
| format | Article |
| id | doaj.art-efd67ecabc6042c4993f1128a2a48eb3 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-12T08:58:48Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-efd67ecabc6042c4993f1128a2a48eb32022-12-22T00:29:54ZengFrontiers Media S.A.Frontiers in Immunology1664-32242022-05-011310.3389/fimmu.2022.885782885782Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicusShaoqing Zang0Li-Xia Lv1Chen-Fei Liu2Peng Zhang3Cang Li4Jin-Xing Wang5Jin-Xing Wang6Shandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaShandong Provincial Key Laboratory of Animal Cells and Developmental Biology, School of Life Sciences, Shandong University, Qingdao, ChinaState Key Laboratory of Microbial Technology, Shandong University, Qingdao, ChinaTrained immunity is driven by metabolism and epigenetics in innate immune cells in mammals. The phenomenon of trained immunity has been identified in invertebrates, including shrimp, but the underlying mechanisms remain unclear. To elucidate mechanisms of trained immunity in shrimp, the metabolomic changes in hemolymph of Marsupenaeus japonicus trained by the UV-inactivated white spot syndrome virus (UV-WSSV) were analyzed using tandem gas chromatography–mass/mass spectrometry. The metabolomic profiles of shrimp trained with UV-WSSV followed WSSV infection showed significant differences comparison with the control groups, PBS injection followed WSSV infection. 16 differential metabolites in total of 154 metabolites were identified, including D-fructose-6-phosphate, D-glucose-6-phosphate, and D-fructose-6-phosphate, and metabolic pathways, glycolysis, pentose phosphate pathway, and AMPK signaling pathway were enriched in the UV-WSSV trained groups. Further study found that histone monomethylation and trimethylation at H3K4 (H3K4me1 and H3K4me3) were involved in the trained immunity. Our data suggest that the UV-WSSV induced trained immunity leads to metabolism reprogramming in the shrimp and provide insights for WSSV control in shrimp aquaculture.https://www.frontiersin.org/articles/10.3389/fimmu.2022.885782/fulltrained immunityshrimpWSSVGC–MS/MSmetabolites |
| spellingShingle | Shaoqing Zang Li-Xia Lv Chen-Fei Liu Peng Zhang Cang Li Jin-Xing Wang Jin-Xing Wang Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicus Frontiers in Immunology trained immunity shrimp WSSV GC–MS/MS metabolites |
| title | Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicus |
| title_full | Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicus |
| title_fullStr | Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicus |
| title_full_unstemmed | Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicus |
| title_short | Metabolomic Investigation of Ultraviolet Ray-Inactivated White Spot Syndrome Virus-Induced Trained Immunity in Marsupenaeus japonicus |
| title_sort | metabolomic investigation of ultraviolet ray inactivated white spot syndrome virus induced trained immunity in marsupenaeus japonicus |
| topic | trained immunity shrimp WSSV GC–MS/MS metabolites |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2022.885782/full |
| work_keys_str_mv | AT shaoqingzang metabolomicinvestigationofultravioletrayinactivatedwhitespotsyndromevirusinducedtrainedimmunityinmarsupenaeusjaponicus AT lixialv metabolomicinvestigationofultravioletrayinactivatedwhitespotsyndromevirusinducedtrainedimmunityinmarsupenaeusjaponicus AT chenfeiliu metabolomicinvestigationofultravioletrayinactivatedwhitespotsyndromevirusinducedtrainedimmunityinmarsupenaeusjaponicus AT pengzhang metabolomicinvestigationofultravioletrayinactivatedwhitespotsyndromevirusinducedtrainedimmunityinmarsupenaeusjaponicus AT cangli metabolomicinvestigationofultravioletrayinactivatedwhitespotsyndromevirusinducedtrainedimmunityinmarsupenaeusjaponicus AT jinxingwang metabolomicinvestigationofultravioletrayinactivatedwhitespotsyndromevirusinducedtrainedimmunityinmarsupenaeusjaponicus AT jinxingwang metabolomicinvestigationofultravioletrayinactivatedwhitespotsyndromevirusinducedtrainedimmunityinmarsupenaeusjaponicus |